Experiment: LB 0.1x with Mixed culture; Trichoderma harzianum M10 spores at 2.5*10^6 spores/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS23170 and BWI76_RS23175 are separated by 11 nucleotides BWI76_RS23175 and BWI76_RS23180 overlap by 4 nucleotides
BWI76_RS23170: BWI76_RS23170 - aldehyde dehydrogenase EutE, at 4,826,966 to 4,828,357
_RS23170
BWI76_RS23175: BWI76_RS23175 - propanediol utilization protein, at 4,828,369 to 4,829,481
_RS23175
BWI76_RS23180: BWI76_RS23180 - NADH dehydrogenase, at 4,829,478 to 4,830,833
_RS23180
Position (kb) 4828
4829
4830 Strain fitness (log2 ratio) -1
0
1
2
3 at 4827.389 kb on - strand, within BWI76_RS23170 at 4827.415 kb on - strand, within BWI76_RS23170 at 4827.474 kb on + strand, within BWI76_RS23170 at 4827.482 kb on - strand, within BWI76_RS23170 at 4827.688 kb on + strand, within BWI76_RS23170 at 4828.247 kb on + strand at 4828.469 kb on + strand at 4828.491 kb on + strand, within BWI76_RS23175 at 4828.499 kb on - strand, within BWI76_RS23175 at 4828.579 kb on + strand, within BWI76_RS23175 at 4828.739 kb on + strand, within BWI76_RS23175 at 4828.747 kb on - strand, within BWI76_RS23175 at 4828.784 kb on + strand, within BWI76_RS23175 at 4828.825 kb on - strand, within BWI76_RS23175 at 4829.095 kb on + strand, within BWI76_RS23175 at 4829.168 kb on - strand, within BWI76_RS23175 at 4829.449 kb on - strand at 4829.475 kb on + strand at 4829.483 kb on - strand at 4829.648 kb on + strand, within BWI76_RS23180 at 4829.648 kb on + strand, within BWI76_RS23180 at 4829.689 kb on - strand, within BWI76_RS23180 at 4830.148 kb on - strand, within BWI76_RS23180 at 4830.286 kb on + strand, within BWI76_RS23180 at 4830.324 kb on - strand, within BWI76_RS23180 at 4830.393 kb on + strand, within BWI76_RS23180 at 4830.401 kb on - strand, within BWI76_RS23180
Per-strain Table
Position Strand Gene LocusTag Fraction LB 0.1x with Mixed culture; Trichoderma harzianum M10 spores at 2.5*10^6 spores/mL remove 4,827,389 - BWI76_RS23170 0.30 +0.1 4,827,415 - BWI76_RS23170 0.32 -0.4 4,827,474 + BWI76_RS23170 0.36 +1.1 4,827,482 - BWI76_RS23170 0.37 +0.3 4,827,688 + BWI76_RS23170 0.52 +0.1 4,828,247 + +0.6 4,828,469 + +0.5 4,828,491 + BWI76_RS23175 0.11 +1.3 4,828,499 - BWI76_RS23175 0.12 +0.1 4,828,579 + BWI76_RS23175 0.19 +2.1 4,828,739 + BWI76_RS23175 0.33 +0.4 4,828,747 - BWI76_RS23175 0.34 +0.3 4,828,784 + BWI76_RS23175 0.37 +3.6 4,828,825 - BWI76_RS23175 0.41 -0.4 4,829,095 + BWI76_RS23175 0.65 +0.2 4,829,168 - BWI76_RS23175 0.72 +0.6 4,829,449 - +1.2 4,829,475 + -0.3 4,829,483 - -0.7 4,829,648 + BWI76_RS23180 0.13 -0.3 4,829,648 + BWI76_RS23180 0.13 +0.1 4,829,689 - BWI76_RS23180 0.16 +1.1 4,830,148 - BWI76_RS23180 0.49 +1.1 4,830,286 + BWI76_RS23180 0.60 +0.6 4,830,324 - BWI76_RS23180 0.62 -0.4 4,830,393 + BWI76_RS23180 0.67 -0.3 4,830,401 - BWI76_RS23180 0.68 +0.5
Or see this region's nucleotide sequence