Experiment: LB 0.1x with Mixed culture; Trichoderma harzianum M10 spores at 2.5*10^6 spores/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS22805 and BWI76_RS22810 are separated by 97 nucleotides BWI76_RS22810 and BWI76_RS22815 are separated by 124 nucleotides
BWI76_RS22805: BWI76_RS22805 - GTP diphosphokinase, at 4,754,751 to 4,756,988
_RS22805
BWI76_RS22810: BWI76_RS22810 - 23S rRNA (uracil(1939)-C(5))-methyltransferase, at 4,757,086 to 4,758,429
_RS22810
BWI76_RS22815: BWI76_RS22815 - hybrid sensor histidine kinase/response regulator, at 4,758,554 to 4,761,307
_RS22815
Position (kb) 4757
4758
4759 Strain fitness (log2 ratio) -5
-4
-3
-2
-1
0
1 at 4756.100 kb on + strand, within BWI76_RS22805 at 4756.264 kb on + strand, within BWI76_RS22805 at 4756.303 kb on + strand, within BWI76_RS22805 at 4756.410 kb on - strand, within BWI76_RS22805 at 4756.531 kb on + strand, within BWI76_RS22805 at 4756.598 kb on + strand, within BWI76_RS22805 at 4756.598 kb on + strand, within BWI76_RS22805 at 4756.606 kb on - strand, within BWI76_RS22805 at 4756.661 kb on - strand, within BWI76_RS22805 at 4756.663 kb on + strand, within BWI76_RS22805 at 4756.746 kb on - strand, within BWI76_RS22805 at 4756.775 kb on - strand at 4756.997 kb on + strand at 4756.997 kb on + strand at 4756.997 kb on + strand at 4757.003 kb on - strand at 4757.005 kb on - strand at 4757.005 kb on - strand at 4757.005 kb on - strand at 4757.005 kb on - strand at 4757.042 kb on + strand at 4757.042 kb on - strand at 4757.531 kb on + strand, within BWI76_RS22810 at 4757.637 kb on + strand, within BWI76_RS22810 at 4757.673 kb on + strand, within BWI76_RS22810 at 4757.689 kb on + strand, within BWI76_RS22810 at 4757.945 kb on - strand, within BWI76_RS22810 at 4758.076 kb on - strand, within BWI76_RS22810 at 4758.227 kb on + strand, within BWI76_RS22810 at 4758.227 kb on + strand, within BWI76_RS22810 at 4758.227 kb on + strand, within BWI76_RS22810 at 4758.235 kb on - strand, within BWI76_RS22810 at 4758.377 kb on + strand at 4758.377 kb on + strand at 4758.444 kb on - strand at 4758.490 kb on - strand at 4758.490 kb on - strand at 4758.520 kb on - strand at 4758.561 kb on + strand at 4758.569 kb on - strand at 4758.608 kb on + strand at 4758.672 kb on + strand at 4758.704 kb on - strand at 4758.891 kb on - strand, within BWI76_RS22815 at 4758.959 kb on - strand, within BWI76_RS22815 at 4759.116 kb on - strand, within BWI76_RS22815 at 4759.214 kb on + strand, within BWI76_RS22815 at 4759.234 kb on - strand, within BWI76_RS22815
Per-strain Table
Position Strand Gene LocusTag Fraction LB 0.1x with Mixed culture; Trichoderma harzianum M10 spores at 2.5*10^6 spores/mL remove 4,756,100 + BWI76_RS22805 0.60 -1.1 4,756,264 + BWI76_RS22805 0.68 -2.2 4,756,303 + BWI76_RS22805 0.69 -5.0 4,756,410 - BWI76_RS22805 0.74 -1.6 4,756,531 + BWI76_RS22805 0.80 -4.4 4,756,598 + BWI76_RS22805 0.83 -3.2 4,756,598 + BWI76_RS22805 0.83 -3.2 4,756,606 - BWI76_RS22805 0.83 -4.0 4,756,661 - BWI76_RS22805 0.85 -2.1 4,756,663 + BWI76_RS22805 0.85 -2.0 4,756,746 - BWI76_RS22805 0.89 -1.0 4,756,775 - -2.0 4,756,997 + -0.1 4,756,997 + -1.6 4,756,997 + -2.8 4,757,003 - +0.2 4,757,005 - -0.3 4,757,005 - -2.0 4,757,005 - -1.9 4,757,005 - -1.8 4,757,042 + -4.2 4,757,042 - +1.5 4,757,531 + BWI76_RS22810 0.33 -0.8 4,757,637 + BWI76_RS22810 0.41 -0.7 4,757,673 + BWI76_RS22810 0.44 +0.1 4,757,689 + BWI76_RS22810 0.45 -4.7 4,757,945 - BWI76_RS22810 0.64 -2.8 4,758,076 - BWI76_RS22810 0.74 -0.6 4,758,227 + BWI76_RS22810 0.85 +0.3 4,758,227 + BWI76_RS22810 0.85 -2.7 4,758,227 + BWI76_RS22810 0.85 -3.3 4,758,235 - BWI76_RS22810 0.85 -0.9 4,758,377 + +0.6 4,758,377 + -0.3 4,758,444 - -0.4 4,758,490 - +0.0 4,758,490 - +0.0 4,758,520 - +0.7 4,758,561 + -1.3 4,758,569 - +0.2 4,758,608 + +0.0 4,758,672 + -1.4 4,758,704 - +0.2 4,758,891 - BWI76_RS22815 0.12 -1.0 4,758,959 - BWI76_RS22815 0.15 -3.8 4,759,116 - BWI76_RS22815 0.20 -0.6 4,759,214 + BWI76_RS22815 0.24 +0.7 4,759,234 - BWI76_RS22815 0.25 -1.4
Or see this region's nucleotide sequence