Experiment: LB 0.1x with Mixed culture; Trichoderma harzianum M10 spores at 2.5*10^6 spores/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS22780 and BWI76_RS22785 are separated by 459 nucleotides BWI76_RS22785 and BWI76_RS22790 are separated by 67 nucleotides
BWI76_RS22780: BWI76_RS22780 - 7-carboxy-7-deazaguanine synthase QueE, at 4,748,348 to 4,749,019
_RS22780
BWI76_RS22785: BWI76_RS22785 - NAD(P)H-binding protein, at 4,749,479 to 4,750,588
_RS22785
BWI76_RS22790: BWI76_RS22790 - phosphopyruvate hydratase, at 4,750,656 to 4,751,954
_RS22790
Position (kb) 4749
4750
4751 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3 at 4748.605 kb on + strand, within BWI76_RS22780 at 4748.613 kb on - strand, within BWI76_RS22780 at 4748.627 kb on + strand, within BWI76_RS22780 at 4748.635 kb on - strand, within BWI76_RS22780 at 4748.635 kb on - strand, within BWI76_RS22780 at 4748.774 kb on - strand, within BWI76_RS22780 at 4748.779 kb on - strand, within BWI76_RS22780 at 4748.833 kb on + strand, within BWI76_RS22780 at 4748.884 kb on + strand, within BWI76_RS22780 at 4748.972 kb on + strand at 4748.974 kb on - strand at 4748.987 kb on + strand at 4748.988 kb on + strand at 4748.996 kb on - strand at 4749.014 kb on - strand at 4749.030 kb on + strand at 4749.068 kb on + strand at 4749.073 kb on + strand at 4749.073 kb on + strand at 4749.079 kb on + strand at 4749.081 kb on - strand at 4749.082 kb on - strand at 4749.084 kb on + strand at 4749.118 kb on + strand at 4749.126 kb on - strand at 4749.128 kb on + strand at 4749.134 kb on - strand at 4749.134 kb on - strand at 4749.156 kb on - strand at 4749.196 kb on + strand at 4749.204 kb on - strand at 4749.204 kb on - strand at 4749.238 kb on + strand at 4749.238 kb on + strand at 4749.238 kb on + strand at 4749.246 kb on - strand at 4749.246 kb on - strand at 4749.246 kb on - strand at 4749.246 kb on - strand at 4749.305 kb on - strand at 4749.305 kb on - strand at 4749.330 kb on + strand at 4749.350 kb on - strand at 4749.389 kb on - strand at 4749.459 kb on + strand at 4749.467 kb on - strand at 4749.473 kb on + strand at 4749.517 kb on + strand at 4749.517 kb on + strand at 4749.517 kb on + strand at 4749.522 kb on + strand at 4749.522 kb on + strand at 4749.527 kb on + strand at 4749.527 kb on + strand at 4749.527 kb on + strand at 4749.527 kb on + strand at 4749.527 kb on + strand at 4749.527 kb on + strand at 4749.527 kb on + strand at 4749.535 kb on - strand at 4749.535 kb on - strand at 4749.572 kb on + strand at 4749.572 kb on - strand at 4749.572 kb on - strand at 4749.574 kb on - strand at 4749.580 kb on - strand at 4749.580 kb on - strand at 4749.587 kb on + strand at 4749.594 kb on + strand, within BWI76_RS22785 at 4749.608 kb on + strand, within BWI76_RS22785 at 4749.612 kb on + strand, within BWI76_RS22785 at 4749.613 kb on + strand, within BWI76_RS22785 at 4749.613 kb on + strand, within BWI76_RS22785 at 4749.619 kb on + strand, within BWI76_RS22785 at 4749.619 kb on + strand, within BWI76_RS22785 at 4749.626 kb on - strand, within BWI76_RS22785 at 4749.626 kb on - strand, within BWI76_RS22785 at 4749.662 kb on + strand, within BWI76_RS22785 at 4749.662 kb on + strand, within BWI76_RS22785 at 4749.664 kb on + strand, within BWI76_RS22785 at 4749.675 kb on + strand, within BWI76_RS22785 at 4749.679 kb on - strand, within BWI76_RS22785 at 4749.681 kb on - strand, within BWI76_RS22785 at 4749.686 kb on - strand, within BWI76_RS22785 at 4749.691 kb on - strand, within BWI76_RS22785 at 4749.752 kb on - strand, within BWI76_RS22785 at 4749.757 kb on - strand, within BWI76_RS22785 at 4749.847 kb on + strand, within BWI76_RS22785 at 4749.897 kb on - strand, within BWI76_RS22785 at 4749.897 kb on - strand, within BWI76_RS22785 at 4750.121 kb on - strand, within BWI76_RS22785 at 4750.432 kb on - strand, within BWI76_RS22785 at 4750.467 kb on + strand, within BWI76_RS22785
Per-strain Table
Position Strand Gene LocusTag Fraction LB 0.1x with Mixed culture; Trichoderma harzianum M10 spores at 2.5*10^6 spores/mL remove 4,748,605 + BWI76_RS22780 0.38 +0.2 4,748,613 - BWI76_RS22780 0.39 +0.6 4,748,627 + BWI76_RS22780 0.42 +1.1 4,748,635 - BWI76_RS22780 0.43 +0.5 4,748,635 - BWI76_RS22780 0.43 +0.4 4,748,774 - BWI76_RS22780 0.63 +0.7 4,748,779 - BWI76_RS22780 0.64 +0.4 4,748,833 + BWI76_RS22780 0.72 -1.0 4,748,884 + BWI76_RS22780 0.80 -0.9 4,748,972 + -2.7 4,748,974 - +0.4 4,748,987 + +0.2 4,748,988 + +0.6 4,748,996 - -0.1 4,749,014 - +1.6 4,749,030 + +3.3 4,749,068 + +1.6 4,749,073 + +0.0 4,749,073 + -1.0 4,749,079 + -1.0 4,749,081 - -0.3 4,749,082 - +0.4 4,749,084 + +1.0 4,749,118 + -1.7 4,749,126 - +0.8 4,749,128 + +0.1 4,749,134 - -3.1 4,749,134 - -1.3 4,749,156 - +0.4 4,749,196 + +1.0 4,749,204 - +0.8 4,749,204 - +1.2 4,749,238 + -0.5 4,749,238 + +0.3 4,749,238 + -2.6 4,749,246 - -1.0 4,749,246 - +0.6 4,749,246 - -1.5 4,749,246 - +1.0 4,749,305 - -1.2 4,749,305 - +2.8 4,749,330 + +1.1 4,749,350 - +0.3 4,749,389 - +0.1 4,749,459 + -4.1 4,749,467 - +1.0 4,749,473 + +0.3 4,749,517 + -1.8 4,749,517 + -0.6 4,749,517 + +2.8 4,749,522 + -0.8 4,749,522 + -1.0 4,749,527 + -1.6 4,749,527 + -0.6 4,749,527 + -3.0 4,749,527 + -0.5 4,749,527 + -0.9 4,749,527 + -0.7 4,749,527 + -3.3 4,749,535 - +0.0 4,749,535 - +0.6 4,749,572 + +0.3 4,749,572 - +0.0 4,749,572 - -3.5 4,749,574 - +0.3 4,749,580 - -2.6 4,749,580 - -3.9 4,749,587 + +0.1 4,749,594 + BWI76_RS22785 0.10 -0.8 4,749,608 + BWI76_RS22785 0.12 -1.3 4,749,612 + BWI76_RS22785 0.12 -2.7 4,749,613 + BWI76_RS22785 0.12 -1.4 4,749,613 + BWI76_RS22785 0.12 +0.1 4,749,619 + BWI76_RS22785 0.13 +0.1 4,749,619 + BWI76_RS22785 0.13 -1.4 4,749,626 - BWI76_RS22785 0.13 +0.0 4,749,626 - BWI76_RS22785 0.13 -0.4 4,749,662 + BWI76_RS22785 0.16 -1.3 4,749,662 + BWI76_RS22785 0.16 +0.9 4,749,664 + BWI76_RS22785 0.17 -0.7 4,749,675 + BWI76_RS22785 0.18 +1.0 4,749,679 - BWI76_RS22785 0.18 +0.0 4,749,681 - BWI76_RS22785 0.18 +0.0 4,749,686 - BWI76_RS22785 0.19 -3.4 4,749,691 - BWI76_RS22785 0.19 -2.3 4,749,752 - BWI76_RS22785 0.25 -0.2 4,749,757 - BWI76_RS22785 0.25 -2.0 4,749,847 + BWI76_RS22785 0.33 -2.4 4,749,897 - BWI76_RS22785 0.38 -1.3 4,749,897 - BWI76_RS22785 0.38 +1.2 4,750,121 - BWI76_RS22785 0.58 -0.1 4,750,432 - BWI76_RS22785 0.86 +1.4 4,750,467 + BWI76_RS22785 0.89 -0.6
Or see this region's nucleotide sequence