Experiment: LB 0.1x with Mixed culture; Trichoderma harzianum M10 spores at 2.5*10^6 spores/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS22460 and BWI76_RS22465 overlap by 4 nucleotides BWI76_RS22465 and BWI76_RS22470 overlap by 1 nucleotides BWI76_RS22470 and BWI76_RS22475 overlap by 8 nucleotides
BWI76_RS22460: BWI76_RS22460 - hemin-degrading factor, at 4,683,073 to 4,684,113
_RS22460
BWI76_RS22465: BWI76_RS22465 - hemin ABC transporter substrate-binding protein, at 4,684,110 to 4,684,928
_RS22465
BWI76_RS22470: BWI76_RS22470 - transporter permease, at 4,684,928 to 4,685,923
_RS22470
BWI76_RS22475: BWI76_RS22475 - heme ABC transporter ATP-binding protein, at 4,685,916 to 4,686,695
_RS22475
Position (kb) 4684
4685 Strain fitness (log2 ratio) -1
0
1
2 at 4683.383 kb on + strand at 4683.500 kb on - strand, within BWI76_RS22460 at 4683.555 kb on + strand, within BWI76_RS22460 at 4683.563 kb on - strand, within BWI76_RS22460 at 4683.674 kb on - strand, within BWI76_RS22460 at 4683.840 kb on - strand, within BWI76_RS22460 at 4683.947 kb on - strand, within BWI76_RS22460 at 4684.023 kb on + strand at 4684.030 kb on + strand at 4684.031 kb on - strand at 4684.031 kb on - strand at 4684.067 kb on + strand at 4684.280 kb on - strand, within BWI76_RS22465 at 4684.360 kb on - strand at 4684.406 kb on + strand, within BWI76_RS22465 at 4684.519 kb on + strand, within BWI76_RS22465 at 4684.521 kb on - strand, within BWI76_RS22465 at 4684.663 kb on + strand, within BWI76_RS22465 at 4684.810 kb on - strand, within BWI76_RS22465 at 4684.872 kb on - strand at 4685.003 kb on - strand at 4685.172 kb on - strand, within BWI76_RS22470 at 4685.404 kb on + strand, within BWI76_RS22470 at 4685.513 kb on + strand, within BWI76_RS22470 at 4685.798 kb on + strand, within BWI76_RS22470
Per-strain Table
Position Strand Gene LocusTag Fraction LB 0.1x with Mixed culture; Trichoderma harzianum M10 spores at 2.5*10^6 spores/mL remove 4,683,383 + +2.0 4,683,500 - BWI76_RS22460 0.41 +0.1 4,683,555 + BWI76_RS22460 0.46 +1.0 4,683,563 - BWI76_RS22460 0.47 +0.7 4,683,674 - BWI76_RS22460 0.58 -0.3 4,683,840 - BWI76_RS22460 0.74 -0.9 4,683,947 - BWI76_RS22460 0.84 -1.0 4,684,023 + -1.6 4,684,030 + -0.3 4,684,031 - +0.6 4,684,031 - -1.4 4,684,067 + +1.2 4,684,280 - BWI76_RS22465 0.21 -1.4 4,684,360 - +1.0 4,684,406 + BWI76_RS22465 0.36 -0.1 4,684,519 + BWI76_RS22465 0.50 +0.3 4,684,521 - BWI76_RS22465 0.50 -1.3 4,684,663 + BWI76_RS22465 0.68 -0.5 4,684,810 - BWI76_RS22465 0.85 -0.9 4,684,872 - +0.3 4,685,003 - +0.0 4,685,172 - BWI76_RS22470 0.24 +0.3 4,685,404 + BWI76_RS22470 0.48 -0.1 4,685,513 + BWI76_RS22470 0.59 -1.3 4,685,798 + BWI76_RS22470 0.87 +0.3
Or see this region's nucleotide sequence