Experiment: LB 0.1x with Mixed culture; Trichoderma harzianum M10 spores at 2.5*10^6 spores/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS20460 and BWI76_RS20465 are separated by 160 nucleotides BWI76_RS20465 and BWI76_RS20470 are separated by 372 nucleotides BWI76_RS20470 and BWI76_RS20475 are separated by 62 nucleotides
BWI76_RS20460: BWI76_RS20460 - acetyl-CoA C-acyltransferase FadI, at 4,282,960 to 4,284,270
_RS20460
BWI76_RS20465: BWI76_RS20465 - hypothetical protein, at 4,284,431 to 4,284,715
_RS20465
BWI76_RS20470: BWI76_RS20470 - long-chain fatty acid transporter, at 4,285,088 to 4,286,452
_RS20470
BWI76_RS20475: BWI76_RS20475 - phospholipid-binding lipoprotein MlaA, at 4,286,515 to 4,287,276
_RS20475
Position (kb) 4285
4286
4287 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 4284.142 kb on + strand at 4284.172 kb on + strand at 4284.220 kb on - strand at 4284.263 kb on - strand at 4284.280 kb on + strand at 4284.401 kb on - strand at 4284.496 kb on + strand, within BWI76_RS20465 at 4284.509 kb on + strand, within BWI76_RS20465 at 4284.525 kb on - strand, within BWI76_RS20465 at 4284.532 kb on - strand at 4284.675 kb on - strand, within BWI76_RS20465 at 4284.694 kb on + strand at 4284.704 kb on + strand at 4284.704 kb on + strand at 4284.965 kb on + strand at 4284.978 kb on - strand at 4284.980 kb on - strand at 4284.997 kb on - strand at 4285.024 kb on - strand at 4285.156 kb on - strand at 4285.156 kb on - strand at 4285.161 kb on - strand at 4285.328 kb on - strand, within BWI76_RS20470 at 4285.397 kb on - strand, within BWI76_RS20470 at 4285.936 kb on - strand, within BWI76_RS20470 at 4285.936 kb on - strand, within BWI76_RS20470 at 4286.048 kb on + strand, within BWI76_RS20470 at 4286.057 kb on + strand, within BWI76_RS20470 at 4286.161 kb on - strand, within BWI76_RS20470 at 4286.189 kb on - strand, within BWI76_RS20470 at 4286.558 kb on + strand at 4286.807 kb on - strand, within BWI76_RS20475 at 4287.003 kb on + strand, within BWI76_RS20475 at 4287.101 kb on + strand, within BWI76_RS20475 at 4287.126 kb on - strand, within BWI76_RS20475 at 4287.163 kb on + strand, within BWI76_RS20475
Per-strain Table
Position Strand Gene LocusTag Fraction LB 0.1x with Mixed culture; Trichoderma harzianum M10 spores at 2.5*10^6 spores/mL remove 4,284,142 + +0.9 4,284,172 + -1.8 4,284,220 - -0.6 4,284,263 - -0.9 4,284,280 + +0.1 4,284,401 - +1.1 4,284,496 + BWI76_RS20465 0.23 +1.0 4,284,509 + BWI76_RS20465 0.27 +0.4 4,284,525 - BWI76_RS20465 0.33 -1.0 4,284,532 - +1.0 4,284,675 - BWI76_RS20465 0.86 +0.2 4,284,694 + -2.4 4,284,704 + -2.0 4,284,704 + +1.9 4,284,965 + +0.5 4,284,978 - -2.6 4,284,980 - +0.0 4,284,997 - +2.2 4,285,024 - -0.5 4,285,156 - -0.6 4,285,156 - -0.3 4,285,161 - -3.3 4,285,328 - BWI76_RS20470 0.18 +0.2 4,285,397 - BWI76_RS20470 0.23 +0.0 4,285,936 - BWI76_RS20470 0.62 -2.0 4,285,936 - BWI76_RS20470 0.62 -1.0 4,286,048 + BWI76_RS20470 0.70 +0.2 4,286,057 + BWI76_RS20470 0.71 +0.3 4,286,161 - BWI76_RS20470 0.79 -1.5 4,286,189 - BWI76_RS20470 0.81 -1.9 4,286,558 + +1.9 4,286,807 - BWI76_RS20475 0.38 +0.0 4,287,003 + BWI76_RS20475 0.64 -2.9 4,287,101 + BWI76_RS20475 0.77 +2.5 4,287,126 - BWI76_RS20475 0.80 -1.0 4,287,163 + BWI76_RS20475 0.85 +1.0
Or see this region's nucleotide sequence