Experiment: LB 0.1x with Mixed culture; Trichoderma harzianum M10 spores at 2.5*10^6 spores/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS19555 and BWI76_RS19560 are separated by 165 nucleotides BWI76_RS19560 and BWI76_RS19565 are separated by 152 nucleotides
BWI76_RS19555: BWI76_RS19555 - Beta-glucoside phosphotransferase system, IIA/IIB/IIC components <small>(from data)</small>, at 4,087,643 to 4,089,502
_RS19555
BWI76_RS19560: BWI76_RS19560 - beta-1,4-xylanase, at 4,089,668 to 4,090,501
_RS19560
BWI76_RS19565: BWI76_RS19565 - tRNA dihydrouridine(16) synthase DusC, at 4,090,654 to 4,091,592
_RS19565
Position (kb) 4089
4090
4091 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 4088.833 kb on + strand, within BWI76_RS19555 at 4089.122 kb on + strand, within BWI76_RS19555 at 4089.130 kb on + strand, within BWI76_RS19555 at 4089.167 kb on - strand, within BWI76_RS19555 at 4089.430 kb on + strand at 4089.438 kb on - strand at 4089.470 kb on + strand at 4089.479 kb on - strand at 4089.545 kb on - strand at 4089.782 kb on + strand, within BWI76_RS19560 at 4089.866 kb on - strand, within BWI76_RS19560 at 4089.985 kb on - strand, within BWI76_RS19560 at 4090.013 kb on - strand, within BWI76_RS19560 at 4090.071 kb on - strand, within BWI76_RS19560 at 4090.120 kb on + strand, within BWI76_RS19560 at 4090.156 kb on - strand, within BWI76_RS19560 at 4090.224 kb on - strand, within BWI76_RS19560 at 4090.303 kb on + strand, within BWI76_RS19560 at 4090.432 kb on - strand at 4090.436 kb on - strand at 4090.596 kb on - strand at 4090.715 kb on + strand at 4090.723 kb on - strand at 4090.723 kb on - strand at 4090.733 kb on + strand at 4090.856 kb on - strand, within BWI76_RS19565 at 4090.858 kb on + strand, within BWI76_RS19565 at 4090.906 kb on + strand, within BWI76_RS19565 at 4091.197 kb on + strand, within BWI76_RS19565 at 4091.311 kb on - strand, within BWI76_RS19565 at 4091.436 kb on + strand, within BWI76_RS19565 at 4091.444 kb on - strand, within BWI76_RS19565
Per-strain Table
Position Strand Gene LocusTag Fraction LB 0.1x with Mixed culture; Trichoderma harzianum M10 spores at 2.5*10^6 spores/mL remove 4,088,833 + BWI76_RS19555 0.64 -3.6 4,089,122 + BWI76_RS19555 0.80 -1.7 4,089,130 + BWI76_RS19555 0.80 +0.6 4,089,167 - BWI76_RS19555 0.82 +0.5 4,089,430 + +0.2 4,089,438 - +1.3 4,089,470 + -1.8 4,089,479 - +0.0 4,089,545 - -2.8 4,089,782 + BWI76_RS19560 0.14 +1.0 4,089,866 - BWI76_RS19560 0.24 -3.0 4,089,985 - BWI76_RS19560 0.38 +1.4 4,090,013 - BWI76_RS19560 0.41 +1.0 4,090,071 - BWI76_RS19560 0.48 +0.3 4,090,120 + BWI76_RS19560 0.54 -2.2 4,090,156 - BWI76_RS19560 0.59 +0.8 4,090,224 - BWI76_RS19560 0.67 -0.3 4,090,303 + BWI76_RS19560 0.76 +2.2 4,090,432 - -2.4 4,090,436 - -3.6 4,090,596 - +1.6 4,090,715 + -0.3 4,090,723 - -0.0 4,090,723 - +1.7 4,090,733 + -1.0 4,090,856 - BWI76_RS19565 0.22 +0.9 4,090,858 + BWI76_RS19565 0.22 -0.1 4,090,906 + BWI76_RS19565 0.27 -0.5 4,091,197 + BWI76_RS19565 0.58 +0.4 4,091,311 - BWI76_RS19565 0.70 +0.9 4,091,436 + BWI76_RS19565 0.83 -0.4 4,091,444 - BWI76_RS19565 0.84 -0.3
Or see this region's nucleotide sequence