Experiment: LB 0.1x with Mixed culture; Trichoderma harzianum M10 spores at 2.5*10^6 spores/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS17830 and BWI76_RS17835 are separated by 10 nucleotides BWI76_RS17835 and BWI76_RS17840 are separated by 9 nucleotides BWI76_RS17840 and BWI76_RS17845 are separated by 11 nucleotides
BWI76_RS17830: BWI76_RS17830 - ABC transporter ATP-binding protein, at 3,706,113 to 3,707,207
_RS17830
BWI76_RS17835: BWI76_RS17835 - sugar ABC transporter permease, at 3,707,218 to 3,708,057
_RS17835
BWI76_RS17840: BWI76_RS17840 - sugar ABC transporter permease, at 3,708,067 to 3,709,008
_RS17840
BWI76_RS17845: BWI76_RS17845 - extracellular solute-binding protein, at 3,709,020 to 3,710,309
_RS17845
Position (kb) 3708
3709
3710 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1 at 3707.192 kb on + strand at 3707.397 kb on - strand, within BWI76_RS17835 at 3707.530 kb on - strand, within BWI76_RS17835 at 3707.588 kb on - strand, within BWI76_RS17835 at 3707.643 kb on - strand, within BWI76_RS17835 at 3707.704 kb on - strand, within BWI76_RS17835 at 3707.866 kb on + strand, within BWI76_RS17835 at 3707.961 kb on - strand, within BWI76_RS17835 at 3707.997 kb on - strand at 3708.210 kb on + strand, within BWI76_RS17840 at 3708.218 kb on + strand, within BWI76_RS17840 at 3708.297 kb on - strand, within BWI76_RS17840 at 3708.516 kb on + strand, within BWI76_RS17840 at 3708.659 kb on + strand, within BWI76_RS17840 at 3708.719 kb on + strand, within BWI76_RS17840 at 3708.761 kb on - strand, within BWI76_RS17840 at 3708.797 kb on - strand, within BWI76_RS17840 at 3708.797 kb on - strand, within BWI76_RS17840 at 3708.923 kb on + strand at 3709.330 kb on - strand, within BWI76_RS17845 at 3709.423 kb on + strand, within BWI76_RS17845 at 3709.431 kb on - strand, within BWI76_RS17845 at 3709.431 kb on - strand, within BWI76_RS17845 at 3709.431 kb on - strand, within BWI76_RS17845 at 3709.458 kb on + strand, within BWI76_RS17845 at 3709.469 kb on + strand, within BWI76_RS17845 at 3709.472 kb on - strand, within BWI76_RS17845 at 3709.504 kb on + strand, within BWI76_RS17845 at 3709.953 kb on + strand, within BWI76_RS17845 at 3709.961 kb on - strand, within BWI76_RS17845
Per-strain Table
Position Strand Gene LocusTag Fraction LB 0.1x with Mixed culture; Trichoderma harzianum M10 spores at 2.5*10^6 spores/mL remove 3,707,192 + -2.7 3,707,397 - BWI76_RS17835 0.21 -1.3 3,707,530 - BWI76_RS17835 0.37 -0.4 3,707,588 - BWI76_RS17835 0.44 -1.1 3,707,643 - BWI76_RS17835 0.51 -0.7 3,707,704 - BWI76_RS17835 0.58 -2.6 3,707,866 + BWI76_RS17835 0.77 -1.7 3,707,961 - BWI76_RS17835 0.88 -2.4 3,707,997 - -0.6 3,708,210 + BWI76_RS17840 0.15 +0.7 3,708,218 + BWI76_RS17840 0.16 +0.3 3,708,297 - BWI76_RS17840 0.24 +1.1 3,708,516 + BWI76_RS17840 0.48 -0.0 3,708,659 + BWI76_RS17840 0.63 -0.6 3,708,719 + BWI76_RS17840 0.69 +0.0 3,708,761 - BWI76_RS17840 0.74 +0.9 3,708,797 - BWI76_RS17840 0.77 -0.2 3,708,797 - BWI76_RS17840 0.77 -1.9 3,708,923 + +0.5 3,709,330 - BWI76_RS17845 0.24 -0.1 3,709,423 + BWI76_RS17845 0.31 +0.6 3,709,431 - BWI76_RS17845 0.32 +1.0 3,709,431 - BWI76_RS17845 0.32 -1.2 3,709,431 - BWI76_RS17845 0.32 +0.1 3,709,458 + BWI76_RS17845 0.34 +0.5 3,709,469 + BWI76_RS17845 0.35 -1.0 3,709,472 - BWI76_RS17845 0.35 -2.3 3,709,504 + BWI76_RS17845 0.38 -3.9 3,709,953 + BWI76_RS17845 0.72 +0.6 3,709,961 - BWI76_RS17845 0.73 +0.1
Or see this region's nucleotide sequence