Experiment: LB 0.1x with Mixed culture; Trichoderma harzianum M10 spores at 2.5*10^6 spores/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS17825 and BWI76_RS17830 are separated by 7 nucleotides BWI76_RS17830 and BWI76_RS17835 are separated by 10 nucleotides BWI76_RS17835 and BWI76_RS17840 are separated by 9 nucleotides
BWI76_RS17825: BWI76_RS17825 - alginate lyase, at 3,704,966 to 3,706,105
_RS17825
BWI76_RS17830: BWI76_RS17830 - ABC transporter ATP-binding protein, at 3,706,113 to 3,707,207
_RS17830
BWI76_RS17835: BWI76_RS17835 - sugar ABC transporter permease, at 3,707,218 to 3,708,057
_RS17835
BWI76_RS17840: BWI76_RS17840 - sugar ABC transporter permease, at 3,708,067 to 3,709,008
_RS17840
Position (kb)
3706
3707
3708 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 3705.136 kb on - strand, within BWI76_RS17825 at 3705.520 kb on + strand, within BWI76_RS17825 at 3705.763 kb on - strand, within BWI76_RS17825 at 3705.780 kb on - strand, within BWI76_RS17825 at 3705.861 kb on + strand, within BWI76_RS17825 at 3705.939 kb on - strand, within BWI76_RS17825 at 3706.011 kb on + strand at 3706.035 kb on + strand at 3706.272 kb on - strand, within BWI76_RS17830 at 3706.345 kb on + strand, within BWI76_RS17830 at 3706.353 kb on - strand, within BWI76_RS17830 at 3706.355 kb on + strand, within BWI76_RS17830 at 3706.377 kb on - strand, within BWI76_RS17830 at 3706.647 kb on - strand, within BWI76_RS17830 at 3706.665 kb on + strand, within BWI76_RS17830 at 3706.885 kb on + strand, within BWI76_RS17830 at 3706.913 kb on - strand, within BWI76_RS17830 at 3706.996 kb on - strand, within BWI76_RS17830 at 3707.192 kb on + strand at 3707.397 kb on - strand, within BWI76_RS17835 at 3707.530 kb on - strand, within BWI76_RS17835 at 3707.588 kb on - strand, within BWI76_RS17835 at 3707.643 kb on - strand, within BWI76_RS17835 at 3707.704 kb on - strand, within BWI76_RS17835 at 3707.866 kb on + strand, within BWI76_RS17835 at 3707.961 kb on - strand, within BWI76_RS17835 at 3707.997 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB 0.1x with Mixed culture; Trichoderma harzianum M10 spores at 2.5*10^6 spores/mL remove 3,705,136 - BWI76_RS17825 0.15 +0.8 3,705,520 + BWI76_RS17825 0.49 -0.1 3,705,763 - BWI76_RS17825 0.70 +0.3 3,705,780 - BWI76_RS17825 0.71 +0.1 3,705,861 + BWI76_RS17825 0.79 -1.7 3,705,939 - BWI76_RS17825 0.85 +0.3 3,706,011 + +0.7 3,706,035 + -0.3 3,706,272 - BWI76_RS17830 0.15 -1.7 3,706,345 + BWI76_RS17830 0.21 -0.6 3,706,353 - BWI76_RS17830 0.22 +1.3 3,706,355 + BWI76_RS17830 0.22 +0.9 3,706,377 - BWI76_RS17830 0.24 +1.9 3,706,647 - BWI76_RS17830 0.49 -2.6 3,706,665 + BWI76_RS17830 0.50 +0.2 3,706,885 + BWI76_RS17830 0.71 +0.3 3,706,913 - BWI76_RS17830 0.73 +0.2 3,706,996 - BWI76_RS17830 0.81 -0.3 3,707,192 + -2.7 3,707,397 - BWI76_RS17835 0.21 -1.3 3,707,530 - BWI76_RS17835 0.37 -0.4 3,707,588 - BWI76_RS17835 0.44 -1.1 3,707,643 - BWI76_RS17835 0.51 -0.7 3,707,704 - BWI76_RS17835 0.58 -2.6 3,707,866 + BWI76_RS17835 0.77 -1.7 3,707,961 - BWI76_RS17835 0.88 -2.4 3,707,997 - -0.6
Or see this region's nucleotide sequence