Experiment: LB 0.1x with Mixed culture; Trichoderma harzianum M10 spores at 2.5*10^6 spores/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS17525 and BWI76_RS17530 overlap by 96 nucleotides BWI76_RS17530 and BWI76_RS17535 are separated by 251 nucleotides BWI76_RS17535 and BWI76_RS17540 overlap by 4 nucleotides
BWI76_RS17525: BWI76_RS17525 - XshC-Cox1-family protein, at 3,634,823 to 3,635,656
_RS17525
BWI76_RS17530: BWI76_RS17530 - hypothetical protein, at 3,635,561 to 3,635,938
_RS17530
BWI76_RS17535: BWI76_RS17535 - rRNA metabolism protein, at 3,636,190 to 3,639,294
_RS17535
BWI76_RS17540: BWI76_RS17540 - chlorohydrolase/aminohydrolase, at 3,639,291 to 3,640,628
_RS17540
Position (kb)
3636
3637
3638
3639
3640 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 3635.433 kb on - strand, within BWI76_RS17525 at 3635.549 kb on + strand, within BWI76_RS17525 at 3635.563 kb on + strand at 3635.571 kb on - strand at 3635.582 kb on + strand at 3635.590 kb on - strand at 3635.603 kb on + strand at 3635.613 kb on - strand at 3635.615 kb on + strand at 3635.623 kb on - strand at 3635.623 kb on - strand at 3635.623 kb on - strand at 3635.623 kb on - strand at 3635.631 kb on - strand at 3635.670 kb on - strand, within BWI76_RS17530 at 3635.671 kb on - strand, within BWI76_RS17530 at 3635.685 kb on + strand, within BWI76_RS17530 at 3635.765 kb on + strand, within BWI76_RS17530 at 3635.853 kb on + strand, within BWI76_RS17530 at 3635.884 kb on + strand, within BWI76_RS17530 at 3635.974 kb on + strand at 3635.982 kb on - strand at 3635.982 kb on - strand at 3636.000 kb on + strand at 3636.051 kb on + strand at 3636.059 kb on - strand at 3636.133 kb on + strand at 3636.147 kb on - strand at 3636.173 kb on + strand at 3636.175 kb on + strand at 3636.186 kb on + strand at 3636.249 kb on + strand at 3636.249 kb on + strand at 3636.249 kb on + strand at 3636.257 kb on - strand at 3636.331 kb on + strand at 3636.344 kb on - strand at 3636.349 kb on - strand at 3636.393 kb on - strand at 3636.432 kb on + strand at 3636.440 kb on - strand at 3636.474 kb on + strand at 3636.501 kb on + strand, within BWI76_RS17535 at 3636.555 kb on + strand, within BWI76_RS17535 at 3636.563 kb on - strand, within BWI76_RS17535 at 3636.587 kb on + strand, within BWI76_RS17535 at 3636.599 kb on - strand, within BWI76_RS17535 at 3636.599 kb on - strand, within BWI76_RS17535 at 3636.665 kb on + strand, within BWI76_RS17535 at 3636.665 kb on + strand, within BWI76_RS17535 at 3636.713 kb on + strand, within BWI76_RS17535 at 3636.747 kb on - strand, within BWI76_RS17535 at 3636.832 kb on - strand, within BWI76_RS17535 at 3636.852 kb on + strand, within BWI76_RS17535 at 3636.860 kb on - strand, within BWI76_RS17535 at 3636.970 kb on + strand, within BWI76_RS17535 at 3637.008 kb on - strand, within BWI76_RS17535 at 3637.234 kb on - strand, within BWI76_RS17535 at 3637.339 kb on - strand, within BWI76_RS17535 at 3637.557 kb on + strand, within BWI76_RS17535 at 3637.717 kb on + strand, within BWI76_RS17535 at 3638.062 kb on + strand, within BWI76_RS17535 at 3638.070 kb on - strand, within BWI76_RS17535 at 3638.141 kb on + strand, within BWI76_RS17535 at 3638.156 kb on + strand, within BWI76_RS17535 at 3638.198 kb on + strand, within BWI76_RS17535 at 3638.330 kb on + strand, within BWI76_RS17535 at 3638.444 kb on + strand, within BWI76_RS17535 at 3638.627 kb on - strand, within BWI76_RS17535 at 3638.721 kb on + strand, within BWI76_RS17535 at 3638.901 kb on - strand, within BWI76_RS17535 at 3639.033 kb on + strand at 3639.266 kb on + strand at 3639.405 kb on - strand at 3639.443 kb on - strand, within BWI76_RS17540 at 3639.611 kb on - strand, within BWI76_RS17540 at 3639.708 kb on - strand, within BWI76_RS17540 at 3639.871 kb on - strand at 3639.929 kb on - strand, within BWI76_RS17540 at 3639.940 kb on - strand, within BWI76_RS17540 at 3639.999 kb on + strand, within BWI76_RS17540 at 3640.092 kb on + strand, within BWI76_RS17540 at 3640.121 kb on + strand, within BWI76_RS17540 at 3640.172 kb on - strand, within BWI76_RS17540 at 3640.267 kb on - strand, within BWI76_RS17540
Per-strain Table
Position Strand Gene LocusTag Fraction LB 0.1x with Mixed culture; Trichoderma harzianum M10 spores at 2.5*10^6 spores/mL remove 3,635,433 - BWI76_RS17525 0.73 +1.5 3,635,549 + BWI76_RS17525 0.87 -0.8 3,635,563 + +0.1 3,635,571 - +1.5 3,635,582 + +0.0 3,635,590 - +0.1 3,635,603 + -2.0 3,635,613 - +0.3 3,635,615 + +0.8 3,635,623 - -2.2 3,635,623 - -1.0 3,635,623 - +0.8 3,635,623 - -0.6 3,635,631 - -0.2 3,635,670 - BWI76_RS17530 0.29 +1.2 3,635,671 - BWI76_RS17530 0.29 +0.8 3,635,685 + BWI76_RS17530 0.33 -0.2 3,635,765 + BWI76_RS17530 0.54 -2.2 3,635,853 + BWI76_RS17530 0.77 +0.2 3,635,884 + BWI76_RS17530 0.85 +2.4 3,635,974 + -1.9 3,635,982 - -0.3 3,635,982 - +0.1 3,636,000 + +0.6 3,636,051 + +0.8 3,636,059 - +2.3 3,636,133 + -0.9 3,636,147 - -1.5 3,636,173 + +0.4 3,636,175 + +0.7 3,636,186 + -0.2 3,636,249 + -0.2 3,636,249 + -1.5 3,636,249 + -1.5 3,636,257 - -2.9 3,636,331 + -3.3 3,636,344 - -0.3 3,636,349 - -2.8 3,636,393 - -1.8 3,636,432 + +1.0 3,636,440 - +2.1 3,636,474 + +1.1 3,636,501 + BWI76_RS17535 0.10 -2.0 3,636,555 + BWI76_RS17535 0.12 +1.0 3,636,563 - BWI76_RS17535 0.12 -3.6 3,636,587 + BWI76_RS17535 0.13 -1.6 3,636,599 - BWI76_RS17535 0.13 +0.7 3,636,599 - BWI76_RS17535 0.13 -0.8 3,636,665 + BWI76_RS17535 0.15 +0.3 3,636,665 + BWI76_RS17535 0.15 +1.2 3,636,713 + BWI76_RS17535 0.17 +0.8 3,636,747 - BWI76_RS17535 0.18 -2.8 3,636,832 - BWI76_RS17535 0.21 -0.2 3,636,852 + BWI76_RS17535 0.21 +0.2 3,636,860 - BWI76_RS17535 0.22 +1.3 3,636,970 + BWI76_RS17535 0.25 +2.2 3,637,008 - BWI76_RS17535 0.26 +0.4 3,637,234 - BWI76_RS17535 0.34 -0.8 3,637,339 - BWI76_RS17535 0.37 +0.3 3,637,557 + BWI76_RS17535 0.44 +1.1 3,637,717 + BWI76_RS17535 0.49 -0.0 3,638,062 + BWI76_RS17535 0.60 +0.9 3,638,070 - BWI76_RS17535 0.61 +0.8 3,638,141 + BWI76_RS17535 0.63 -3.5 3,638,156 + BWI76_RS17535 0.63 -0.1 3,638,198 + BWI76_RS17535 0.65 -0.4 3,638,330 + BWI76_RS17535 0.69 +0.8 3,638,444 + BWI76_RS17535 0.73 +0.5 3,638,627 - BWI76_RS17535 0.78 +0.2 3,638,721 + BWI76_RS17535 0.82 +0.8 3,638,901 - BWI76_RS17535 0.87 +0.5 3,639,033 + -1.9 3,639,266 + +0.3 3,639,405 - +0.8 3,639,443 - BWI76_RS17540 0.11 +2.0 3,639,611 - BWI76_RS17540 0.24 +0.5 3,639,708 - BWI76_RS17540 0.31 +0.9 3,639,871 - -1.2 3,639,929 - BWI76_RS17540 0.48 +0.4 3,639,940 - BWI76_RS17540 0.49 +1.6 3,639,999 + BWI76_RS17540 0.53 -0.2 3,640,092 + BWI76_RS17540 0.60 +0.9 3,640,121 + BWI76_RS17540 0.62 +0.8 3,640,172 - BWI76_RS17540 0.66 -0.3 3,640,267 - BWI76_RS17540 0.73 +0.6
Or see this region's nucleotide sequence