Experiment: LB 0.1x with Mixed culture; Trichoderma harzianum M10 spores at 2.5*10^6 spores/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS13750 and BWI76_RS13755 are separated by 41 nucleotides BWI76_RS13755 and BWI76_RS13760 overlap by 1 nucleotides BWI76_RS13760 and BWI76_RS13765 are separated by 356 nucleotides BWI76_RS13765 and BWI76_RS13770 are separated by 5 nucleotides
BWI76_RS13750: BWI76_RS13750 - formate dehydrogenase, at 2,868,274 to 2,868,930
_RS13750
BWI76_RS13755: BWI76_RS13755 - hypothetical protein, at 2,868,972 to 2,869,295
_RS13755
BWI76_RS13760: BWI76_RS13760 - hypothetical protein, at 2,869,295 to 2,869,483
_RS13760
BWI76_RS13765: BWI76_RS13765 - MFS transporter AraJ, at 2,869,840 to 2,870,991
_RS13765
BWI76_RS13770: BWI76_RS13770 - alkylhydroperoxidase domain protein, at 2,870,997 to 2,872,088
_RS13770
Position (kb)
2869
2870
2871 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 2868.865 kb on - strand at 2868.883 kb on + strand at 2868.886 kb on - strand at 2868.931 kb on + strand at 2868.960 kb on + strand at 2868.968 kb on - strand at 2868.970 kb on + strand at 2868.978 kb on - strand at 2869.189 kb on - strand, within BWI76_RS13755 at 2869.191 kb on + strand, within BWI76_RS13755 at 2869.264 kb on + strand at 2869.265 kb on + strand at 2869.293 kb on + strand at 2869.303 kb on + strand at 2869.310 kb on + strand at 2869.310 kb on - strand at 2869.387 kb on - strand, within BWI76_RS13760 at 2869.392 kb on - strand at 2869.393 kb on + strand, within BWI76_RS13760 at 2869.435 kb on - strand at 2869.460 kb on + strand, within BWI76_RS13760 at 2869.468 kb on - strand at 2869.503 kb on - strand at 2869.549 kb on + strand at 2869.549 kb on + strand at 2869.549 kb on + strand at 2869.554 kb on + strand at 2869.557 kb on - strand at 2869.562 kb on - strand at 2869.581 kb on - strand at 2869.656 kb on + strand at 2869.661 kb on + strand at 2869.663 kb on + strand at 2869.664 kb on - strand at 2869.666 kb on + strand at 2869.669 kb on - strand at 2869.669 kb on - strand at 2869.679 kb on - strand at 2869.679 kb on - strand at 2869.993 kb on + strand, within BWI76_RS13765 at 2870.009 kb on + strand, within BWI76_RS13765 at 2870.014 kb on + strand, within BWI76_RS13765 at 2870.101 kb on + strand, within BWI76_RS13765 at 2870.288 kb on - strand, within BWI76_RS13765 at 2870.288 kb on - strand, within BWI76_RS13765 at 2870.342 kb on - strand, within BWI76_RS13765 at 2870.347 kb on + strand, within BWI76_RS13765 at 2870.352 kb on + strand, within BWI76_RS13765 at 2870.355 kb on - strand, within BWI76_RS13765 at 2870.355 kb on - strand, within BWI76_RS13765 at 2870.472 kb on - strand, within BWI76_RS13765 at 2870.501 kb on + strand, within BWI76_RS13765 at 2870.501 kb on - strand, within BWI76_RS13765 at 2870.700 kb on + strand, within BWI76_RS13765 at 2870.700 kb on + strand, within BWI76_RS13765 at 2870.708 kb on - strand, within BWI76_RS13765 at 2870.708 kb on - strand, within BWI76_RS13765 at 2870.708 kb on - strand, within BWI76_RS13765 at 2870.743 kb on + strand, within BWI76_RS13765 at 2870.765 kb on - strand, within BWI76_RS13765 at 2870.806 kb on + strand, within BWI76_RS13765 at 2870.906 kb on + strand at 2871.274 kb on + strand, within BWI76_RS13770 at 2871.753 kb on - strand, within BWI76_RS13770 at 2871.838 kb on + strand, within BWI76_RS13770
Per-strain Table
Position Strand Gene LocusTag Fraction LB 0.1x with Mixed culture; Trichoderma harzianum M10 spores at 2.5*10^6 spores/mL remove 2,868,865 - +1.5 2,868,883 + +0.4 2,868,886 - +0.0 2,868,931 + -0.1 2,868,960 + -0.7 2,868,968 - -2.3 2,868,970 + -0.6 2,868,978 - -3.5 2,869,189 - BWI76_RS13755 0.67 -0.8 2,869,191 + BWI76_RS13755 0.68 -2.4 2,869,264 + -0.8 2,869,265 + -0.1 2,869,293 + -1.4 2,869,303 + +0.6 2,869,310 + +0.5 2,869,310 - +2.3 2,869,387 - BWI76_RS13760 0.49 -2.1 2,869,392 - -0.7 2,869,393 + BWI76_RS13760 0.52 -2.3 2,869,435 - +1.5 2,869,460 + BWI76_RS13760 0.87 -2.7 2,869,468 - -1.6 2,869,503 - +0.9 2,869,549 + +0.8 2,869,549 + -1.4 2,869,549 + +3.7 2,869,554 + +0.9 2,869,557 - -3.4 2,869,562 - -2.1 2,869,581 - +0.3 2,869,656 + -2.4 2,869,661 + -1.4 2,869,663 + -0.2 2,869,664 - +2.7 2,869,666 + +0.9 2,869,669 - -0.7 2,869,669 - -2.6 2,869,679 - -1.2 2,869,679 - +0.6 2,869,993 + BWI76_RS13765 0.13 -0.1 2,870,009 + BWI76_RS13765 0.15 -2.1 2,870,014 + BWI76_RS13765 0.15 +0.6 2,870,101 + BWI76_RS13765 0.23 -3.0 2,870,288 - BWI76_RS13765 0.39 -2.4 2,870,288 - BWI76_RS13765 0.39 +1.4 2,870,342 - BWI76_RS13765 0.44 -2.7 2,870,347 + BWI76_RS13765 0.44 +0.1 2,870,352 + BWI76_RS13765 0.44 -0.8 2,870,355 - BWI76_RS13765 0.45 -2.8 2,870,355 - BWI76_RS13765 0.45 -3.0 2,870,472 - BWI76_RS13765 0.55 +2.5 2,870,501 + BWI76_RS13765 0.57 +1.9 2,870,501 - BWI76_RS13765 0.57 -0.1 2,870,700 + BWI76_RS13765 0.75 -2.4 2,870,700 + BWI76_RS13765 0.75 -1.4 2,870,708 - BWI76_RS13765 0.75 +0.9 2,870,708 - BWI76_RS13765 0.75 -1.9 2,870,708 - BWI76_RS13765 0.75 -3.5 2,870,743 + BWI76_RS13765 0.78 +0.2 2,870,765 - BWI76_RS13765 0.80 -0.7 2,870,806 + BWI76_RS13765 0.84 -0.2 2,870,906 + +0.0 2,871,274 + BWI76_RS13770 0.25 -1.7 2,871,753 - BWI76_RS13770 0.69 +0.4 2,871,838 + BWI76_RS13770 0.77 -0.4
Or see this region's nucleotide sequence