Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS10660

Experiment: LB 0.1x with Mixed culture; Trichoderma harzianum M10 spores at 2.5*10^6 spores/mL

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS10650 and BWI76_RS10655 are separated by 15 nucleotidesBWI76_RS10655 and BWI76_RS10660 overlap by 35 nucleotidesBWI76_RS10660 and BWI76_RS10665 are separated by 51 nucleotidesBWI76_RS10665 and BWI76_RS10670 are separated by 20 nucleotides BWI76_RS10650: BWI76_RS10650 - PTS fructose-like transporter subunit EIIB, at 2,248,865 to 2,249,206 _RS10650 BWI76_RS10655: BWI76_RS10655 - [formate-C-acetyltransferase]-activating enzyme, at 2,249,222 to 2,250,097 _RS10655 BWI76_RS10660: BWI76_RS10660 - formate C-acetyltransferase/glycerol dehydratase family glycyl radical enzyme, at 2,250,063 to 2,252,360 _RS10660 BWI76_RS10665: BWI76_RS10665 - PTS system fructose-like transporter subunit EIIB, at 2,252,412 to 2,252,732 _RS10665 BWI76_RS10670: BWI76_RS10670 - PTS fructose transporter subunit IIC, at 2,252,753 to 2,253,829 _RS10670 Position (kb) 2250 2251 2252 2253Strain fitness (log2 ratio) -3 -2 -1 0 1at 2249.148 kb on + strand, within BWI76_RS10650at 2249.150 kb on + strand, within BWI76_RS10650at 2249.156 kb on - strand, within BWI76_RS10650at 2249.156 kb on - strand, within BWI76_RS10650at 2249.389 kb on + strand, within BWI76_RS10655at 2249.389 kb on + strand, within BWI76_RS10655at 2249.389 kb on + strand, within BWI76_RS10655at 2249.397 kb on - strand, within BWI76_RS10655at 2249.397 kb on - strand, within BWI76_RS10655at 2250.094 kb on - strandat 2250.097 kb on + strandat 2250.243 kb on - strandat 2250.342 kb on - strand, within BWI76_RS10660at 2250.378 kb on - strand, within BWI76_RS10660at 2250.881 kb on - strand, within BWI76_RS10660at 2250.931 kb on + strand, within BWI76_RS10660at 2250.993 kb on - strand, within BWI76_RS10660at 2251.003 kb on - strand, within BWI76_RS10660at 2251.246 kb on - strand, within BWI76_RS10660at 2251.384 kb on - strand, within BWI76_RS10660at 2251.606 kb on - strand, within BWI76_RS10660at 2251.744 kb on - strand, within BWI76_RS10660at 2251.746 kb on + strand, within BWI76_RS10660at 2251.766 kb on - strand, within BWI76_RS10660at 2251.904 kb on - strand, within BWI76_RS10660at 2251.904 kb on - strand, within BWI76_RS10660at 2251.948 kb on + strand, within BWI76_RS10660at 2252.107 kb on + strand, within BWI76_RS10660at 2252.677 kb on + strand, within BWI76_RS10665at 2252.682 kb on + strand, within BWI76_RS10665at 2252.685 kb on - strand, within BWI76_RS10665at 2252.790 kb on + strandat 2252.823 kb on + strandat 2252.841 kb on - strandat 2252.934 kb on + strand, within BWI76_RS10670at 2252.934 kb on + strand, within BWI76_RS10670at 2252.980 kb on - strand, within BWI76_RS10670at 2253.276 kb on + strand, within BWI76_RS10670

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Per-strain Table

Position Strand Gene LocusTag Fraction LB 0.1x with Mixed culture; Trichoderma harzianum M10 spores at 2.5*10^6 spores/mL
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2,249,148 + BWI76_RS10650 0.83 -1.6
2,249,150 + BWI76_RS10650 0.83 -0.0
2,249,156 - BWI76_RS10650 0.85 -3.4
2,249,156 - BWI76_RS10650 0.85 -0.2
2,249,389 + BWI76_RS10655 0.19 -2.6
2,249,389 + BWI76_RS10655 0.19 -2.5
2,249,389 + BWI76_RS10655 0.19 +0.4
2,249,397 - BWI76_RS10655 0.20 -2.1
2,249,397 - BWI76_RS10655 0.20 +0.2
2,250,094 - -0.2
2,250,097 + +0.2
2,250,243 - -0.3
2,250,342 - BWI76_RS10660 0.12 -0.4
2,250,378 - BWI76_RS10660 0.14 -0.5
2,250,881 - BWI76_RS10660 0.36 +0.8
2,250,931 + BWI76_RS10660 0.38 +1.4
2,250,993 - BWI76_RS10660 0.40 -0.7
2,251,003 - BWI76_RS10660 0.41 -0.4
2,251,246 - BWI76_RS10660 0.51 +0.9
2,251,384 - BWI76_RS10660 0.57 -1.2
2,251,606 - BWI76_RS10660 0.67 -0.2
2,251,744 - BWI76_RS10660 0.73 -1.6
2,251,746 + BWI76_RS10660 0.73 +0.6
2,251,766 - BWI76_RS10660 0.74 +1.0
2,251,904 - BWI76_RS10660 0.80 -0.6
2,251,904 - BWI76_RS10660 0.80 +0.8
2,251,948 + BWI76_RS10660 0.82 +0.4
2,252,107 + BWI76_RS10660 0.89 -0.3
2,252,677 + BWI76_RS10665 0.83 -1.2
2,252,682 + BWI76_RS10665 0.84 -1.0
2,252,685 - BWI76_RS10665 0.85 +0.1
2,252,790 + -0.0
2,252,823 + +0.7
2,252,841 - +1.0
2,252,934 + BWI76_RS10670 0.17 +0.7
2,252,934 + BWI76_RS10670 0.17 +0.7
2,252,980 - BWI76_RS10670 0.21 -0.1
2,253,276 + BWI76_RS10670 0.49 -3.0

Or see this region's nucleotide sequence