Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS10650

Experiment: LB 0.1x with Mixed culture; Trichoderma harzianum M10 spores at 2.5*10^6 spores/mL

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS10645 and BWI76_RS10650 are separated by 125 nucleotidesBWI76_RS10650 and BWI76_RS10655 are separated by 15 nucleotidesBWI76_RS10655 and BWI76_RS10660 overlap by 35 nucleotides BWI76_RS10645: BWI76_RS10645 - phytanoyl-CoA dioxygenase, at 2,247,972 to 2,248,739 _RS10645 BWI76_RS10650: BWI76_RS10650 - PTS fructose-like transporter subunit EIIB, at 2,248,865 to 2,249,206 _RS10650 BWI76_RS10655: BWI76_RS10655 - [formate-C-acetyltransferase]-activating enzyme, at 2,249,222 to 2,250,097 _RS10655 BWI76_RS10660: BWI76_RS10660 - formate C-acetyltransferase/glycerol dehydratase family glycyl radical enzyme, at 2,250,063 to 2,252,360 _RS10660 Position (kb) 2248 2249 2250Strain fitness (log2 ratio) -3 -2 -1 0 1at 2247.922 kb on + strandat 2248.058 kb on + strand, within BWI76_RS10645at 2248.058 kb on + strand, within BWI76_RS10645at 2248.058 kb on + strand, within BWI76_RS10645at 2248.071 kb on - strand, within BWI76_RS10645at 2248.354 kb on + strand, within BWI76_RS10645at 2248.411 kb on + strand, within BWI76_RS10645at 2248.419 kb on - strand, within BWI76_RS10645at 2248.419 kb on - strand, within BWI76_RS10645at 2248.488 kb on + strand, within BWI76_RS10645at 2249.028 kb on + strand, within BWI76_RS10650at 2249.048 kb on - strand, within BWI76_RS10650at 2249.148 kb on + strand, within BWI76_RS10650at 2249.150 kb on + strand, within BWI76_RS10650at 2249.156 kb on - strand, within BWI76_RS10650at 2249.156 kb on - strand, within BWI76_RS10650at 2249.389 kb on + strand, within BWI76_RS10655at 2249.389 kb on + strand, within BWI76_RS10655at 2249.389 kb on + strand, within BWI76_RS10655at 2249.397 kb on - strand, within BWI76_RS10655at 2249.397 kb on - strand, within BWI76_RS10655at 2250.094 kb on - strandat 2250.097 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction LB 0.1x with Mixed culture; Trichoderma harzianum M10 spores at 2.5*10^6 spores/mL
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2,247,922 + +0.1
2,248,058 + BWI76_RS10645 0.11 -1.0
2,248,058 + BWI76_RS10645 0.11 -2.4
2,248,058 + BWI76_RS10645 0.11 -3.2
2,248,071 - BWI76_RS10645 0.13 -2.2
2,248,354 + BWI76_RS10645 0.50 +0.4
2,248,411 + BWI76_RS10645 0.57 -0.1
2,248,419 - BWI76_RS10645 0.58 +0.1
2,248,419 - BWI76_RS10645 0.58 +0.3
2,248,488 + BWI76_RS10645 0.67 +1.0
2,249,028 + BWI76_RS10650 0.48 +0.2
2,249,048 - BWI76_RS10650 0.54 -1.8
2,249,148 + BWI76_RS10650 0.83 -1.6
2,249,150 + BWI76_RS10650 0.83 -0.0
2,249,156 - BWI76_RS10650 0.85 -3.4
2,249,156 - BWI76_RS10650 0.85 -0.2
2,249,389 + BWI76_RS10655 0.19 -2.6
2,249,389 + BWI76_RS10655 0.19 -2.5
2,249,389 + BWI76_RS10655 0.19 +0.4
2,249,397 - BWI76_RS10655 0.20 -2.1
2,249,397 - BWI76_RS10655 0.20 +0.2
2,250,094 - -0.2
2,250,097 + +0.2

Or see this region's nucleotide sequence