Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS09555

Experiment: LB 0.1x with Mixed culture; Trichoderma harzianum M10 spores at 2.5*10^6 spores/mL

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS09550 and BWI76_RS09555 are separated by 154 nucleotidesBWI76_RS09555 and BWI76_RS09560 are separated by 151 nucleotidesBWI76_RS09560 and BWI76_RS09565 are separated by 39 nucleotides BWI76_RS09550: BWI76_RS09550 - low-specificity L-threonine aldolase, at 1,997,268 to 1,998,269 _RS09550 BWI76_RS09555: BWI76_RS09555 - pyruvate oxidase, at 1,998,424 to 2,000,142 _RS09555 BWI76_RS09560: BWI76_RS09560 - DoxX family membrane protein, at 2,000,294 to 2,000,728 _RS09560 BWI76_RS09565: BWI76_RS09565 - hybrid-cluster NAD(P)-dependent oxidoreductase, at 2,000,768 to 2,001,736 _RS09565 Position (kb) 1998 1999 2000 2001Strain fitness (log2 ratio) -2 -1 0 1at 1997.556 kb on - strand, within BWI76_RS09550at 1997.744 kb on - strand, within BWI76_RS09550at 1997.750 kb on - strand, within BWI76_RS09550at 1998.198 kb on - strandat 1998.235 kb on + strandat 1998.310 kb on - strandat 1998.320 kb on + strandat 1998.492 kb on + strandat 1998.919 kb on - strand, within BWI76_RS09555at 1999.118 kb on - strand, within BWI76_RS09555at 1999.411 kb on - strand, within BWI76_RS09555at 1999.717 kb on + strand, within BWI76_RS09555at 2000.108 kb on - strandat 2000.178 kb on + strandat 2000.441 kb on - strand, within BWI76_RS09560at 2000.602 kb on - strand, within BWI76_RS09560at 2000.916 kb on - strand, within BWI76_RS09565at 2000.965 kb on + strand, within BWI76_RS09565

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Per-strain Table

Position Strand Gene LocusTag Fraction LB 0.1x with Mixed culture; Trichoderma harzianum M10 spores at 2.5*10^6 spores/mL
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1,997,556 - BWI76_RS09550 0.29 +0.3
1,997,744 - BWI76_RS09550 0.48 +0.2
1,997,750 - BWI76_RS09550 0.48 +0.8
1,998,198 - +0.2
1,998,235 + +0.1
1,998,310 - -0.0
1,998,320 + -1.3
1,998,492 + -2.3
1,998,919 - BWI76_RS09555 0.29 -0.1
1,999,118 - BWI76_RS09555 0.40 +0.7
1,999,411 - BWI76_RS09555 0.57 +1.1
1,999,717 + BWI76_RS09555 0.75 +1.2
2,000,108 - -0.3
2,000,178 + -1.3
2,000,441 - BWI76_RS09560 0.34 +0.8
2,000,602 - BWI76_RS09560 0.71 -2.7
2,000,916 - BWI76_RS09565 0.15 +0.1
2,000,965 + BWI76_RS09565 0.20 +0.5

Or see this region's nucleotide sequence