Experiment: LB 0.1x with Mixed culture; Trichoderma harzianum M10 spores at 2.5*10^6 spores/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS07260 and BWI76_RS07265 are separated by 27 nucleotides BWI76_RS07265 and BWI76_RS07270 overlap by 8 nucleotides BWI76_RS07270 and BWI76_RS07275 overlap by 4 nucleotides
BWI76_RS07260: BWI76_RS07260 - branched chain amino acid ABC transporter substrate-binding protein, at 1,527,758 to 1,528,915
_RS07260
BWI76_RS07265: BWI76_RS07265 - ABC transporter ATP-binding protein, at 1,528,943 to 1,529,662
_RS07265
BWI76_RS07270: BWI76_RS07270 - ABC transporter ATP-binding protein, at 1,529,655 to 1,530,422
_RS07270
BWI76_RS07275: BWI76_RS07275 - ABC transporter, at 1,530,419 to 1,531,525
_RS07275
Position (kb) 1528
1529
1530 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1 at 1528.023 kb on + strand, within BWI76_RS07260 at 1528.048 kb on - strand, within BWI76_RS07260 at 1528.503 kb on + strand, within BWI76_RS07260 at 1528.508 kb on + strand, within BWI76_RS07260 at 1528.970 kb on - strand at 1529.017 kb on + strand, within BWI76_RS07265 at 1529.220 kb on - strand, within BWI76_RS07265 at 1529.433 kb on - strand, within BWI76_RS07265 at 1529.539 kb on - strand, within BWI76_RS07265 at 1529.596 kb on - strand at 1529.596 kb on - strand at 1529.890 kb on + strand, within BWI76_RS07270 at 1529.923 kb on - strand, within BWI76_RS07270 at 1530.281 kb on + strand, within BWI76_RS07270 at 1530.289 kb on - strand, within BWI76_RS07270 at 1530.341 kb on + strand, within BWI76_RS07270 at 1530.349 kb on - strand at 1530.447 kb on + strand at 1530.487 kb on + strand at 1530.599 kb on + strand, within BWI76_RS07275
Per-strain Table
Position Strand Gene LocusTag Fraction LB 0.1x with Mixed culture; Trichoderma harzianum M10 spores at 2.5*10^6 spores/mL remove 1,528,023 + BWI76_RS07260 0.23 +0.2 1,528,048 - BWI76_RS07260 0.25 -0.5 1,528,503 + BWI76_RS07260 0.64 -4.2 1,528,508 + BWI76_RS07260 0.65 -0.4 1,528,970 - +0.7 1,529,017 + BWI76_RS07265 0.10 -1.7 1,529,220 - BWI76_RS07265 0.38 -0.8 1,529,433 - BWI76_RS07265 0.68 -0.3 1,529,539 - BWI76_RS07265 0.83 +1.1 1,529,596 - +0.2 1,529,596 - +1.7 1,529,890 + BWI76_RS07270 0.31 -0.5 1,529,923 - BWI76_RS07270 0.35 +0.5 1,530,281 + BWI76_RS07270 0.82 +0.8 1,530,289 - BWI76_RS07270 0.83 -1.8 1,530,341 + BWI76_RS07270 0.89 -1.5 1,530,349 - -1.1 1,530,447 + -0.8 1,530,487 + +0.4 1,530,599 + BWI76_RS07275 0.16 +0.8
Or see this region's nucleotide sequence