Experiment: LB 0.1x with Mixed culture; Trichoderma harzianum M10 spores at 2.5*10^6 spores/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS04310 and BWI76_RS04315 are separated by 505 nucleotides BWI76_RS04315 and BWI76_RS04320 are separated by 53 nucleotides
BWI76_RS04310: BWI76_RS04310 - hypothetical protein, at 888,547 to 888,726
_RS04310
BWI76_RS04315: BWI76_RS04315 - Na+/H+ antiporter NhaA, at 889,232 to 890,407
_RS04315
BWI76_RS04320: BWI76_RS04320 - transcriptional activator NhaR, at 890,461 to 891,360
_RS04320
Position (kb) 889
890
891 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3
4 at 888.375 kb on + strand at 888.395 kb on + strand at 888.395 kb on + strand at 888.403 kb on - strand at 888.403 kb on - strand at 888.403 kb on - strand at 888.403 kb on - strand at 888.403 kb on - strand at 888.403 kb on - strand at 888.438 kb on - strand at 888.438 kb on - strand at 888.547 kb on - strand at 888.547 kb on - strand at 888.547 kb on - strand at 888.574 kb on - strand, within BWI76_RS04310 at 888.642 kb on + strand, within BWI76_RS04310 at 888.642 kb on + strand, within BWI76_RS04310 at 888.642 kb on + strand, within BWI76_RS04310 at 888.642 kb on + strand, within BWI76_RS04310 at 888.642 kb on + strand, within BWI76_RS04310 at 888.645 kb on - strand, within BWI76_RS04310 at 888.647 kb on + strand, within BWI76_RS04310 at 888.650 kb on - strand, within BWI76_RS04310 at 888.650 kb on - strand, within BWI76_RS04310 at 888.650 kb on - strand, within BWI76_RS04310 at 888.650 kb on - strand, within BWI76_RS04310 at 888.650 kb on - strand, within BWI76_RS04310 at 888.689 kb on + strand, within BWI76_RS04310 at 888.689 kb on + strand, within BWI76_RS04310 at 888.707 kb on - strand, within BWI76_RS04310 at 888.707 kb on - strand, within BWI76_RS04310 at 888.734 kb on + strand at 888.757 kb on - strand at 888.792 kb on + strand at 888.810 kb on + strand at 888.811 kb on - strand at 888.818 kb on - strand at 888.841 kb on + strand at 888.841 kb on + strand at 888.849 kb on - strand at 888.849 kb on - strand at 888.849 kb on - strand at 888.854 kb on - strand at 888.854 kb on - strand at 888.856 kb on - strand at 888.950 kb on + strand at 888.950 kb on + strand at 889.089 kb on + strand at 889.303 kb on + strand at 889.315 kb on + strand at 889.330 kb on + strand at 889.370 kb on - strand, within BWI76_RS04315 at 889.466 kb on - strand, within BWI76_RS04315 at 889.508 kb on - strand, within BWI76_RS04315 at 889.508 kb on - strand, within BWI76_RS04315 at 889.603 kb on + strand, within BWI76_RS04315 at 889.655 kb on + strand, within BWI76_RS04315 at 889.750 kb on + strand, within BWI76_RS04315 at 889.750 kb on + strand, within BWI76_RS04315 at 889.750 kb on + strand, within BWI76_RS04315 at 889.750 kb on + strand, within BWI76_RS04315 at 889.758 kb on - strand, within BWI76_RS04315 at 889.813 kb on + strand, within BWI76_RS04315 at 889.851 kb on + strand, within BWI76_RS04315 at 889.851 kb on + strand, within BWI76_RS04315 at 889.866 kb on + strand, within BWI76_RS04315 at 889.874 kb on - strand, within BWI76_RS04315 at 890.085 kb on + strand, within BWI76_RS04315 at 890.139 kb on - strand, within BWI76_RS04315 at 890.189 kb on + strand, within BWI76_RS04315 at 890.220 kb on - strand, within BWI76_RS04315 at 890.286 kb on + strand, within BWI76_RS04315 at 890.292 kb on + strand at 890.395 kb on - strand at 890.467 kb on - strand at 890.544 kb on + strand at 890.624 kb on + strand, within BWI76_RS04320 at 890.880 kb on + strand, within BWI76_RS04320 at 890.914 kb on + strand, within BWI76_RS04320 at 891.025 kb on - strand, within BWI76_RS04320 at 891.371 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB 0.1x with Mixed culture; Trichoderma harzianum M10 spores at 2.5*10^6 spores/mL remove 888,375 + +1.1 888,395 + +0.1 888,395 + +1.1 888,403 - +1.1 888,403 - +0.1 888,403 - +1.1 888,403 - +0.5 888,403 - +1.5 888,403 - +0.6 888,438 - -0.3 888,438 - -1.1 888,547 - -0.4 888,547 - +0.6 888,547 - -1.2 888,574 - BWI76_RS04310 0.15 -2.7 888,642 + BWI76_RS04310 0.53 -1.1 888,642 + BWI76_RS04310 0.53 +0.9 888,642 + BWI76_RS04310 0.53 -0.5 888,642 + BWI76_RS04310 0.53 -0.1 888,642 + BWI76_RS04310 0.53 +0.1 888,645 - BWI76_RS04310 0.54 -1.4 888,647 + BWI76_RS04310 0.56 +1.1 888,650 - BWI76_RS04310 0.57 +0.5 888,650 - BWI76_RS04310 0.57 +1.6 888,650 - BWI76_RS04310 0.57 +1.1 888,650 - BWI76_RS04310 0.57 -1.7 888,650 - BWI76_RS04310 0.57 -0.8 888,689 + BWI76_RS04310 0.79 -1.5 888,689 + BWI76_RS04310 0.79 -2.3 888,707 - BWI76_RS04310 0.89 +1.1 888,707 - BWI76_RS04310 0.89 -1.1 888,734 + +0.7 888,757 - -0.4 888,792 + +1.5 888,810 + -1.0 888,811 - +1.1 888,818 - +0.1 888,841 + -2.0 888,841 + -0.0 888,849 - -0.2 888,849 - -0.8 888,849 - -0.1 888,854 - -0.0 888,854 - +0.1 888,856 - +0.8 888,950 + +0.4 888,950 + +0.4 889,089 + -3.2 889,303 + -2.3 889,315 + -0.0 889,330 + +1.1 889,370 - BWI76_RS04315 0.12 +1.0 889,466 - BWI76_RS04315 0.20 +2.9 889,508 - BWI76_RS04315 0.23 +3.0 889,508 - BWI76_RS04315 0.23 +0.2 889,603 + BWI76_RS04315 0.32 +0.3 889,655 + BWI76_RS04315 0.36 +0.6 889,750 + BWI76_RS04315 0.44 +0.3 889,750 + BWI76_RS04315 0.44 +0.2 889,750 + BWI76_RS04315 0.44 -0.9 889,750 + BWI76_RS04315 0.44 -0.3 889,758 - BWI76_RS04315 0.45 +0.5 889,813 + BWI76_RS04315 0.49 +1.5 889,851 + BWI76_RS04315 0.53 +1.2 889,851 + BWI76_RS04315 0.53 -0.4 889,866 + BWI76_RS04315 0.54 +1.2 889,874 - BWI76_RS04315 0.55 +0.3 890,085 + BWI76_RS04315 0.73 +1.4 890,139 - BWI76_RS04315 0.77 -2.6 890,189 + BWI76_RS04315 0.81 +2.2 890,220 - BWI76_RS04315 0.84 +1.5 890,286 + BWI76_RS04315 0.90 +0.8 890,292 + +4.5 890,395 - +0.2 890,467 - -2.5 890,544 + -3.0 890,624 + BWI76_RS04320 0.18 -3.0 890,880 + BWI76_RS04320 0.47 +0.0 890,914 + BWI76_RS04320 0.50 +0.6 891,025 - BWI76_RS04320 0.63 -0.1 891,371 + +0.6
Or see this region's nucleotide sequence