Experiment: LB 0.1x with Mixed culture; Trichoderma harzianum M10 spores at 2.5*10^6 spores/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS04300 and BWI76_RS04305 are separated by 87 nucleotides BWI76_RS04305 and BWI76_RS04310 are separated by 327 nucleotides
BWI76_RS04300: BWI76_RS04300 - molecular chaperone DnaK, at 885,079 to 886,995
_RS04300
BWI76_RS04305: BWI76_RS04305 - molecular chaperone DnaJ, at 887,083 to 888,219
_RS04305
BWI76_RS04310: BWI76_RS04310 - hypothetical protein, at 888,547 to 888,726
_RS04310
Position (kb) 887
888
889 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2 at 886.916 kb on + strand at 887.035 kb on + strand at 887.041 kb on + strand at 888.082 kb on + strand, within BWI76_RS04305 at 888.108 kb on + strand at 888.108 kb on + strand at 888.128 kb on + strand at 888.128 kb on + strand at 888.128 kb on + strand at 888.128 kb on + strand at 888.128 kb on + strand at 888.128 kb on + strand at 888.136 kb on - strand at 888.145 kb on + strand at 888.146 kb on - strand at 888.148 kb on + strand at 888.153 kb on + strand at 888.153 kb on + strand at 888.158 kb on - strand at 888.166 kb on - strand at 888.205 kb on + strand at 888.207 kb on + strand at 888.375 kb on + strand at 888.395 kb on + strand at 888.395 kb on + strand at 888.403 kb on - strand at 888.403 kb on - strand at 888.403 kb on - strand at 888.403 kb on - strand at 888.403 kb on - strand at 888.403 kb on - strand at 888.438 kb on - strand at 888.438 kb on - strand at 888.547 kb on - strand at 888.547 kb on - strand at 888.547 kb on - strand at 888.574 kb on - strand, within BWI76_RS04310 at 888.642 kb on + strand, within BWI76_RS04310 at 888.642 kb on + strand, within BWI76_RS04310 at 888.642 kb on + strand, within BWI76_RS04310 at 888.642 kb on + strand, within BWI76_RS04310 at 888.642 kb on + strand, within BWI76_RS04310 at 888.645 kb on - strand, within BWI76_RS04310 at 888.647 kb on + strand, within BWI76_RS04310 at 888.650 kb on - strand, within BWI76_RS04310 at 888.650 kb on - strand, within BWI76_RS04310 at 888.650 kb on - strand, within BWI76_RS04310 at 888.650 kb on - strand, within BWI76_RS04310 at 888.650 kb on - strand, within BWI76_RS04310 at 888.689 kb on + strand, within BWI76_RS04310 at 888.689 kb on + strand, within BWI76_RS04310 at 888.707 kb on - strand, within BWI76_RS04310 at 888.707 kb on - strand, within BWI76_RS04310 at 888.734 kb on + strand at 888.757 kb on - strand at 888.792 kb on + strand at 888.810 kb on + strand at 888.811 kb on - strand at 888.818 kb on - strand at 888.841 kb on + strand at 888.841 kb on + strand at 888.849 kb on - strand at 888.849 kb on - strand at 888.849 kb on - strand at 888.854 kb on - strand at 888.854 kb on - strand at 888.856 kb on - strand at 888.950 kb on + strand at 888.950 kb on + strand at 889.089 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB 0.1x with Mixed culture; Trichoderma harzianum M10 spores at 2.5*10^6 spores/mL remove 886,916 + -2.4 887,035 + +1.5 887,041 + -0.8 888,082 + BWI76_RS04305 0.88 -4.0 888,108 + -3.5 888,108 + -2.6 888,128 + +1.9 888,128 + +0.2 888,128 + -0.2 888,128 + +0.6 888,128 + -2.8 888,128 + -0.4 888,136 - -3.6 888,145 + -1.3 888,146 - -0.0 888,148 + -2.4 888,153 + -3.4 888,153 + -4.3 888,158 - -3.0 888,166 - -2.1 888,205 + -0.3 888,207 + -0.3 888,375 + +1.1 888,395 + +0.1 888,395 + +1.1 888,403 - +1.1 888,403 - +0.1 888,403 - +1.1 888,403 - +0.5 888,403 - +1.5 888,403 - +0.6 888,438 - -0.3 888,438 - -1.1 888,547 - -0.4 888,547 - +0.6 888,547 - -1.2 888,574 - BWI76_RS04310 0.15 -2.7 888,642 + BWI76_RS04310 0.53 -1.1 888,642 + BWI76_RS04310 0.53 +0.9 888,642 + BWI76_RS04310 0.53 -0.5 888,642 + BWI76_RS04310 0.53 -0.1 888,642 + BWI76_RS04310 0.53 +0.1 888,645 - BWI76_RS04310 0.54 -1.4 888,647 + BWI76_RS04310 0.56 +1.1 888,650 - BWI76_RS04310 0.57 +0.5 888,650 - BWI76_RS04310 0.57 +1.6 888,650 - BWI76_RS04310 0.57 +1.1 888,650 - BWI76_RS04310 0.57 -1.7 888,650 - BWI76_RS04310 0.57 -0.8 888,689 + BWI76_RS04310 0.79 -1.5 888,689 + BWI76_RS04310 0.79 -2.3 888,707 - BWI76_RS04310 0.89 +1.1 888,707 - BWI76_RS04310 0.89 -1.1 888,734 + +0.7 888,757 - -0.4 888,792 + +1.5 888,810 + -1.0 888,811 - +1.1 888,818 - +0.1 888,841 + -2.0 888,841 + -0.0 888,849 - -0.2 888,849 - -0.8 888,849 - -0.1 888,854 - -0.0 888,854 - +0.1 888,856 - +0.8 888,950 + +0.4 888,950 + +0.4 889,089 + -3.2
Or see this region's nucleotide sequence