Experiment: LB 0.1x with Mixed culture; Trichoderma harzianum M10 spores at 2.5*10^6 spores/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS01995 and BWI76_RS02000 are separated by 84 nucleotides BWI76_RS02000 and BWI76_RS02005 are separated by 258 nucleotides BWI76_RS02005 and BWI76_RS02010 are separated by 36 nucleotides BWI76_RS02010 and BWI76_RS02015 are separated by 151 nucleotides
BWI76_RS01995: BWI76_RS01995 - AraC family transcriptional regulator, at 410,724 to 411,053
_RS01995
BWI76_RS02000: BWI76_RS02000 - transcriptional regulator, at 411,138 to 411,596
_RS02000
BWI76_RS02005: BWI76_RS02005 - glutathione S-transferase, at 411,855 to 412,523
_RS02005
BWI76_RS02010: BWI76_RS02010 - hypothetical protein, at 412,560 to 412,760
_RS02010
BWI76_RS02015: BWI76_RS02015 - NCS2 family permease, at 412,912 to 414,261
_RS02015
Position (kb) 411
412
413 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 410.915 kb on - strand, within BWI76_RS01995 at 411.091 kb on - strand at 411.314 kb on + strand, within BWI76_RS02000 at 411.385 kb on + strand, within BWI76_RS02000 at 411.393 kb on - strand, within BWI76_RS02000 at 411.393 kb on - strand, within BWI76_RS02000 at 411.454 kb on + strand, within BWI76_RS02000 at 411.454 kb on + strand, within BWI76_RS02000 at 411.454 kb on + strand, within BWI76_RS02000 at 411.454 kb on + strand, within BWI76_RS02000 at 411.454 kb on + strand, within BWI76_RS02000 at 411.462 kb on - strand, within BWI76_RS02000 at 411.462 kb on - strand, within BWI76_RS02000 at 411.462 kb on - strand, within BWI76_RS02000 at 411.462 kb on - strand, within BWI76_RS02000 at 411.699 kb on + strand at 411.699 kb on + strand at 411.699 kb on + strand at 411.699 kb on + strand at 411.702 kb on - strand at 411.707 kb on - strand at 411.707 kb on - strand at 411.707 kb on - strand at 411.707 kb on - strand at 411.727 kb on + strand at 411.864 kb on - strand at 411.943 kb on - strand, within BWI76_RS02005 at 411.951 kb on - strand, within BWI76_RS02005 at 411.953 kb on + strand, within BWI76_RS02005 at 411.959 kb on + strand, within BWI76_RS02005 at 412.056 kb on + strand, within BWI76_RS02005 at 412.078 kb on + strand, within BWI76_RS02005 at 412.085 kb on - strand, within BWI76_RS02005 at 412.145 kb on + strand, within BWI76_RS02005 at 412.150 kb on + strand, within BWI76_RS02005 at 412.213 kb on - strand, within BWI76_RS02005 at 412.253 kb on + strand, within BWI76_RS02005 at 412.261 kb on - strand, within BWI76_RS02005 at 412.285 kb on - strand, within BWI76_RS02005 at 412.322 kb on + strand, within BWI76_RS02005 at 412.461 kb on - strand at 412.463 kb on + strand at 412.863 kb on - strand at 413.015 kb on + strand at 413.016 kb on - strand at 413.130 kb on + strand, within BWI76_RS02015 at 413.138 kb on - strand, within BWI76_RS02015 at 413.390 kb on + strand, within BWI76_RS02015
Per-strain Table
Position Strand Gene LocusTag Fraction LB 0.1x with Mixed culture; Trichoderma harzianum M10 spores at 2.5*10^6 spores/mL remove 410,915 - BWI76_RS01995 0.58 +2.1 411,091 - -2.4 411,314 + BWI76_RS02000 0.38 -2.9 411,385 + BWI76_RS02000 0.54 +0.6 411,393 - BWI76_RS02000 0.56 +0.2 411,393 - BWI76_RS02000 0.56 -0.3 411,454 + BWI76_RS02000 0.69 -2.6 411,454 + BWI76_RS02000 0.69 +0.2 411,454 + BWI76_RS02000 0.69 -1.7 411,454 + BWI76_RS02000 0.69 +0.0 411,454 + BWI76_RS02000 0.69 -1.2 411,462 - BWI76_RS02000 0.71 -0.3 411,462 - BWI76_RS02000 0.71 -1.4 411,462 - BWI76_RS02000 0.71 -2.0 411,462 - BWI76_RS02000 0.71 -1.6 411,699 + +0.5 411,699 + -0.5 411,699 + -0.8 411,699 + +1.2 411,702 - -0.4 411,707 - +0.2 411,707 - +0.2 411,707 - +1.4 411,707 - -0.8 411,727 + -0.8 411,864 - -1.1 411,943 - BWI76_RS02005 0.13 -3.6 411,951 - BWI76_RS02005 0.14 +1.2 411,953 + BWI76_RS02005 0.15 -0.9 411,959 + BWI76_RS02005 0.16 +0.5 412,056 + BWI76_RS02005 0.30 -1.1 412,078 + BWI76_RS02005 0.33 -1.6 412,085 - BWI76_RS02005 0.34 -0.2 412,145 + BWI76_RS02005 0.43 -2.3 412,150 + BWI76_RS02005 0.44 -1.4 412,213 - BWI76_RS02005 0.54 +0.7 412,253 + BWI76_RS02005 0.59 +0.2 412,261 - BWI76_RS02005 0.61 -1.4 412,285 - BWI76_RS02005 0.64 -0.6 412,322 + BWI76_RS02005 0.70 -2.1 412,461 - +0.6 412,463 + -3.1 412,863 - +1.1 413,015 + +0.5 413,016 - -0.2 413,130 + BWI76_RS02015 0.16 -0.1 413,138 - BWI76_RS02015 0.17 +0.3 413,390 + BWI76_RS02015 0.35 -0.8
Or see this region's nucleotide sequence