Experiment: LB 0.1x with Mixed culture; Trichoderma harzianum M10 spores at 2.5*10^6 spores/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS01080 and BWI76_RS01085 are separated by 21 nucleotides BWI76_RS01085 and BWI76_RS01090 are separated by 56 nucleotides
BWI76_RS01080: BWI76_RS01080 - undecaprenyl-phosphate alpha-N-acetylglucosaminyl 1-phosphate transferase, at 222,214 to 223,308
_RS01080
BWI76_RS01085: BWI76_RS01085 - polysaccharide chain length modulation protein, at 223,330 to 224,376
_RS01085
BWI76_RS01090: BWI76_RS01090 - UDP-N-acetylglucosamine 2-epimerase (non-hydrolyzing), at 224,433 to 225,563
_RS01090
Position (kb) 223
224
225 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1 at 222.345 kb on - strand, within BWI76_RS01080 at 222.353 kb on - strand, within BWI76_RS01080 at 222.434 kb on + strand, within BWI76_RS01080 at 222.435 kb on + strand, within BWI76_RS01080 at 222.439 kb on + strand, within BWI76_RS01080 at 222.441 kb on - strand, within BWI76_RS01080 at 222.441 kb on - strand, within BWI76_RS01080 at 222.447 kb on - strand, within BWI76_RS01080 at 222.447 kb on - strand, within BWI76_RS01080 at 222.449 kb on + strand, within BWI76_RS01080 at 222.496 kb on - strand, within BWI76_RS01080 at 222.612 kb on - strand, within BWI76_RS01080 at 222.629 kb on + strand, within BWI76_RS01080 at 222.647 kb on - strand, within BWI76_RS01080 at 222.703 kb on + strand, within BWI76_RS01080 at 222.762 kb on - strand, within BWI76_RS01080 at 222.842 kb on + strand, within BWI76_RS01080 at 222.921 kb on + strand, within BWI76_RS01080 at 222.935 kb on + strand, within BWI76_RS01080 at 223.009 kb on - strand, within BWI76_RS01080 at 223.086 kb on + strand, within BWI76_RS01080 at 223.116 kb on - strand, within BWI76_RS01080 at 223.133 kb on + strand, within BWI76_RS01080 at 223.238 kb on + strand at 223.261 kb on - strand at 223.301 kb on - strand at 223.325 kb on - strand at 223.326 kb on + strand at 223.334 kb on - strand at 223.419 kb on + strand at 223.438 kb on + strand, within BWI76_RS01085 at 223.608 kb on + strand, within BWI76_RS01085 at 223.697 kb on + strand, within BWI76_RS01085 at 223.917 kb on + strand, within BWI76_RS01085 at 224.006 kb on + strand, within BWI76_RS01085 at 224.069 kb on + strand, within BWI76_RS01085 at 224.097 kb on + strand, within BWI76_RS01085 at 224.119 kb on - strand, within BWI76_RS01085 at 224.184 kb on - strand, within BWI76_RS01085 at 224.637 kb on - strand, within BWI76_RS01090 at 224.826 kb on - strand, within BWI76_RS01090 at 224.859 kb on + strand, within BWI76_RS01090 at 225.000 kb on - strand, within BWI76_RS01090 at 225.010 kb on + strand, within BWI76_RS01090 at 225.020 kb on + strand, within BWI76_RS01090
Per-strain Table
Position Strand Gene LocusTag Fraction LB 0.1x with Mixed culture; Trichoderma harzianum M10 spores at 2.5*10^6 spores/mL remove 222,345 - BWI76_RS01080 0.12 -2.6 222,353 - BWI76_RS01080 0.13 -2.7 222,434 + BWI76_RS01080 0.20 +0.5 222,435 + BWI76_RS01080 0.20 +1.2 222,439 + BWI76_RS01080 0.21 -1.1 222,441 - BWI76_RS01080 0.21 +0.2 222,441 - BWI76_RS01080 0.21 -2.1 222,447 - BWI76_RS01080 0.21 -4.1 222,447 - BWI76_RS01080 0.21 -0.9 222,449 + BWI76_RS01080 0.21 -3.4 222,496 - BWI76_RS01080 0.26 -0.8 222,612 - BWI76_RS01080 0.36 -1.9 222,629 + BWI76_RS01080 0.38 +0.3 222,647 - BWI76_RS01080 0.40 -1.8 222,703 + BWI76_RS01080 0.45 -2.9 222,762 - BWI76_RS01080 0.50 -4.2 222,842 + BWI76_RS01080 0.57 -3.5 222,921 + BWI76_RS01080 0.65 -1.8 222,935 + BWI76_RS01080 0.66 -4.0 223,009 - BWI76_RS01080 0.73 -2.1 223,086 + BWI76_RS01080 0.80 -3.3 223,116 - BWI76_RS01080 0.82 -2.5 223,133 + BWI76_RS01080 0.84 -2.6 223,238 + -1.4 223,261 - -0.3 223,301 - -3.4 223,325 - -0.2 223,326 + -0.7 223,334 - -3.1 223,419 + +0.2 223,438 + BWI76_RS01085 0.10 -1.6 223,608 + BWI76_RS01085 0.27 -1.1 223,697 + BWI76_RS01085 0.35 -2.6 223,917 + BWI76_RS01085 0.56 -1.0 224,006 + BWI76_RS01085 0.65 -0.5 224,069 + BWI76_RS01085 0.71 -1.5 224,097 + BWI76_RS01085 0.73 -0.3 224,119 - BWI76_RS01085 0.75 -2.1 224,184 - BWI76_RS01085 0.82 -2.8 224,637 - BWI76_RS01090 0.18 +1.2 224,826 - BWI76_RS01090 0.35 -1.5 224,859 + BWI76_RS01090 0.38 -2.8 225,000 - BWI76_RS01090 0.50 -4.4 225,010 + BWI76_RS01090 0.51 -2.3 225,020 + BWI76_RS01090 0.52 -4.3
Or see this region's nucleotide sequence