Experiment: LB 0.1x with Mixed culture; Trichoderma harzianum M10 spores at 2.5*10^6 spores/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS00990 and BWI76_RS00995 are separated by 100 nucleotides BWI76_RS00995 and BWI76_RS01000 are separated by 52 nucleotides BWI76_RS01000 and BWI76_RS01005 are separated by 194 nucleotides BWI76_RS01005 and BWI76_RS01010 are separated by 24 nucleotides BWI76_RS01010 and BWI76_RS01015 overlap by 1 nucleotides
BWI76_RS00990: BWI76_RS00990 - ketol-acid reductoisomerase, at 202,804 to 204,279
_RS00990
BWI76_RS00995: BWI76_RS00995 - peptidyl-prolyl cis-trans isomerase, at 204,380 to 204,661
_RS00995
BWI76_RS01000: BWI76_RS01000 - peptidyl-prolyl cis-trans isomerase, at 204,714 to 204,995
_RS01000
BWI76_RS01005: BWI76_RS01005 - CarD family transcriptional regulator, at 205,190 to 205,894
_RS01005
BWI76_RS01010: BWI76_RS01010 - uridine phosphorylase, at 205,919 to 206,668
_RS01010
BWI76_RS01015: BWI76_RS01015 - nicotinamide riboside transporter PnuC, at 206,668 to 207,390
_RS01015
Position (kb)
205
206 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4
5 at 204.305 kb on + strand at 204.306 kb on + strand at 204.330 kb on + strand at 204.458 kb on + strand, within BWI76_RS00995 at 204.466 kb on - strand, within BWI76_RS00995 at 204.496 kb on - strand, within BWI76_RS00995 at 204.720 kb on - strand at 204.783 kb on - strand, within BWI76_RS01000 at 204.800 kb on - strand at 204.819 kb on + strand, within BWI76_RS01000 at 204.906 kb on + strand, within BWI76_RS01000 at 204.936 kb on - strand, within BWI76_RS01000 at 204.948 kb on + strand, within BWI76_RS01000 at 204.977 kb on - strand at 204.977 kb on - strand at 205.053 kb on + strand at 205.053 kb on + strand at 205.145 kb on + strand at 205.163 kb on + strand at 205.163 kb on + strand at 205.165 kb on + strand at 205.165 kb on + strand at 205.166 kb on - strand at 205.240 kb on + strand at 205.414 kb on + strand, within BWI76_RS01005 at 205.423 kb on - strand, within BWI76_RS01005 at 205.529 kb on + strand, within BWI76_RS01005 at 205.561 kb on - strand, within BWI76_RS01005 at 205.632 kb on - strand, within BWI76_RS01005 at 205.812 kb on - strand, within BWI76_RS01005 at 205.812 kb on - strand, within BWI76_RS01005 at 205.938 kb on - strand at 206.028 kb on + strand, within BWI76_RS01010 at 206.077 kb on + strand, within BWI76_RS01010 at 206.081 kb on - strand, within BWI76_RS01010 at 206.201 kb on + strand, within BWI76_RS01010 at 206.456 kb on + strand, within BWI76_RS01010 at 206.456 kb on + strand, within BWI76_RS01010 at 206.464 kb on - strand, within BWI76_RS01010 at 206.464 kb on - strand, within BWI76_RS01010 at 206.464 kb on - strand, within BWI76_RS01010
Per-strain Table
Position Strand Gene LocusTag Fraction LB 0.1x with Mixed culture; Trichoderma harzianum M10 spores at 2.5*10^6 spores/mL remove 204,305 + +1.0 204,306 + -2.3 204,330 + -0.3 204,458 + BWI76_RS00995 0.28 +0.2 204,466 - BWI76_RS00995 0.30 -0.9 204,496 - BWI76_RS00995 0.41 -0.4 204,720 - +0.6 204,783 - BWI76_RS01000 0.24 +1.1 204,800 - +0.2 204,819 + BWI76_RS01000 0.37 -0.3 204,906 + BWI76_RS01000 0.68 +1.0 204,936 - BWI76_RS01000 0.79 +1.0 204,948 + BWI76_RS01000 0.83 -1.0 204,977 - -1.3 204,977 - +1.0 205,053 + -1.2 205,053 + -3.2 205,145 + +1.2 205,163 + -2.2 205,163 + +0.2 205,165 + -0.3 205,165 + +1.1 205,166 - -0.0 205,240 + +0.7 205,414 + BWI76_RS01005 0.32 -1.3 205,423 - BWI76_RS01005 0.33 -0.2 205,529 + BWI76_RS01005 0.48 +0.2 205,561 - BWI76_RS01005 0.53 -2.2 205,632 - BWI76_RS01005 0.63 -0.6 205,812 - BWI76_RS01005 0.88 -0.0 205,812 - BWI76_RS01005 0.88 -2.3 205,938 - +5.4 206,028 + BWI76_RS01010 0.15 -2.5 206,077 + BWI76_RS01010 0.21 +0.2 206,081 - BWI76_RS01010 0.22 +1.7 206,201 + BWI76_RS01010 0.38 -1.6 206,456 + BWI76_RS01010 0.72 +0.7 206,456 + BWI76_RS01010 0.72 -0.5 206,464 - BWI76_RS01010 0.73 -0.3 206,464 - BWI76_RS01010 0.73 +0.4 206,464 - BWI76_RS01010 0.73 +1.0
Or see this region's nucleotide sequence