Experiment: LB 0.1x with Mixed culture; Trichoderma harzianum M10 spores at 2.5*10^6 spores/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS00465 and BWI76_RS00470 are separated by 48 nucleotides BWI76_RS00470 and BWI76_RS00475 are separated by 156 nucleotides BWI76_RS00475 and BWI76_RS00480 are separated by 128 nucleotides BWI76_RS00480 and BWI76_RS00485 are separated by 144 nucleotides
BWI76_RS00465: BWI76_RS00465 - formate dehydrogenase-N subunit alpha, at 94,761 to 97,175
_RS00465
BWI76_RS00470: BWI76_RS00470 - sulfate ABC transporter substrate-binding protein, at 97,224 to 97,811
_RS00470
BWI76_RS00475: BWI76_RS00475 - sulfurtransferase FdhD, at 97,968 to 98,804
_RS00475
BWI76_RS00480: BWI76_RS00480 - hypothetical protein, at 98,933 to 99,145
_RS00480
BWI76_RS00485: BWI76_RS00485 - beta-xylosidase, at 99,290 to 100,801
_RS00485
Position (kb) 97
98
99 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3
4 at 97.211 kb on + strand at 97.222 kb on + strand at 97.349 kb on + strand, within BWI76_RS00470 at 97.406 kb on + strand, within BWI76_RS00470 at 97.492 kb on - strand, within BWI76_RS00470 at 97.529 kb on + strand, within BWI76_RS00470 at 97.529 kb on + strand, within BWI76_RS00470 at 97.529 kb on + strand, within BWI76_RS00470 at 97.529 kb on + strand, within BWI76_RS00470 at 97.534 kb on + strand, within BWI76_RS00470 at 97.537 kb on - strand, within BWI76_RS00470 at 97.637 kb on + strand, within BWI76_RS00470 at 97.660 kb on + strand, within BWI76_RS00470 at 97.668 kb on - strand, within BWI76_RS00470 at 97.668 kb on - strand, within BWI76_RS00470 at 97.701 kb on - strand, within BWI76_RS00470 at 97.737 kb on + strand, within BWI76_RS00470 at 97.774 kb on + strand at 97.793 kb on + strand at 97.806 kb on - strand at 97.882 kb on - strand at 97.944 kb on - strand at 98.110 kb on - strand, within BWI76_RS00475 at 98.237 kb on - strand, within BWI76_RS00475 at 98.312 kb on + strand, within BWI76_RS00475 at 98.315 kb on - strand, within BWI76_RS00475 at 98.386 kb on + strand, within BWI76_RS00475 at 98.443 kb on + strand, within BWI76_RS00475 at 98.499 kb on - strand, within BWI76_RS00475 at 98.529 kb on - strand, within BWI76_RS00475 at 98.560 kb on - strand, within BWI76_RS00475 at 98.561 kb on - strand, within BWI76_RS00475 at 98.652 kb on + strand, within BWI76_RS00475 at 98.861 kb on - strand at 98.891 kb on - strand at 99.041 kb on - strand, within BWI76_RS00480 at 99.058 kb on + strand, within BWI76_RS00480 at 99.058 kb on + strand, within BWI76_RS00480 at 99.058 kb on + strand, within BWI76_RS00480 at 99.154 kb on - strand at 99.289 kb on - strand at 99.410 kb on + strand at 99.616 kb on + strand, within BWI76_RS00485 at 99.651 kb on + strand, within BWI76_RS00485 at 99.651 kb on + strand, within BWI76_RS00485 at 99.659 kb on - strand, within BWI76_RS00485 at 99.659 kb on - strand, within BWI76_RS00485
Per-strain Table
Position Strand Gene LocusTag Fraction LB 0.1x with Mixed culture; Trichoderma harzianum M10 spores at 2.5*10^6 spores/mL remove 97,211 + -2.9 97,222 + -0.5 97,349 + BWI76_RS00470 0.21 +1.3 97,406 + BWI76_RS00470 0.31 +0.1 97,492 - BWI76_RS00470 0.46 +2.8 97,529 + BWI76_RS00470 0.52 +0.8 97,529 + BWI76_RS00470 0.52 +1.1 97,529 + BWI76_RS00470 0.52 +0.6 97,529 + BWI76_RS00470 0.52 -0.3 97,534 + BWI76_RS00470 0.53 +2.4 97,537 - BWI76_RS00470 0.53 +0.0 97,637 + BWI76_RS00470 0.70 +1.0 97,660 + BWI76_RS00470 0.74 -0.3 97,668 - BWI76_RS00470 0.76 +4.1 97,668 - BWI76_RS00470 0.76 +2.2 97,701 - BWI76_RS00470 0.81 +2.1 97,737 + BWI76_RS00470 0.87 +0.4 97,774 + +0.2 97,793 + +1.1 97,806 - +0.2 97,882 - +1.1 97,944 - +0.8 98,110 - BWI76_RS00475 0.17 -0.3 98,237 - BWI76_RS00475 0.32 +1.5 98,312 + BWI76_RS00475 0.41 +1.6 98,315 - BWI76_RS00475 0.41 -0.0 98,386 + BWI76_RS00475 0.50 +1.0 98,443 + BWI76_RS00475 0.57 +0.8 98,499 - BWI76_RS00475 0.63 +0.2 98,529 - BWI76_RS00475 0.67 +0.7 98,560 - BWI76_RS00475 0.71 +0.5 98,561 - BWI76_RS00475 0.71 -1.6 98,652 + BWI76_RS00475 0.82 +0.2 98,861 - -0.1 98,891 - -2.1 99,041 - BWI76_RS00480 0.51 -0.5 99,058 + BWI76_RS00480 0.59 -1.3 99,058 + BWI76_RS00480 0.59 -0.5 99,058 + BWI76_RS00480 0.59 +2.2 99,154 - -0.3 99,289 - +1.2 99,410 + -0.2 99,616 + BWI76_RS00485 0.22 -1.1 99,651 + BWI76_RS00485 0.24 +0.6 99,651 + BWI76_RS00485 0.24 -2.9 99,659 - BWI76_RS00485 0.24 -2.9 99,659 - BWI76_RS00485 0.24 -2.9
Or see this region's nucleotide sequence