Strain Fitness in Pseudomonas fluorescens FW300-N2E2 around Pf6N2E2_4714

Experiment: Nickel (II) chloride 1 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf6N2E2_4713 and Pf6N2E2_4714 are separated by 217 nucleotidesPf6N2E2_4714 and Pf6N2E2_4715 are separated by 153 nucleotides Pf6N2E2_4713 - GbcA Glycine betaine demethylase subunit A, at 5,448,838 to 5,450,133 _4713 Pf6N2E2_4714 - GbcB Glycine betaine demethylase subunit B, at 5,450,351 to 5,451,517 _4714 Pf6N2E2_4715 - Low-specificity L-threonine aldolase (EC 4.1.2.48), at 5,451,671 to 5,452,711 _4715 Position (kb) 5450 5451 5452Strain fitness (log2 ratio) -2 -1 0 1at 5449.487 kb on + strand, within Pf6N2E2_4713at 5449.487 kb on + strand, within Pf6N2E2_4713at 5449.488 kb on - strand, within Pf6N2E2_4713at 5449.577 kb on + strand, within Pf6N2E2_4713at 5449.578 kb on - strand, within Pf6N2E2_4713at 5449.589 kb on + strand, within Pf6N2E2_4713at 5449.589 kb on + strand, within Pf6N2E2_4713at 5449.590 kb on - strand, within Pf6N2E2_4713at 5449.590 kb on - strand, within Pf6N2E2_4713at 5449.590 kb on - strand, within Pf6N2E2_4713at 5449.590 kb on - strand, within Pf6N2E2_4713at 5449.590 kb on - strand, within Pf6N2E2_4713at 5449.655 kb on + strand, within Pf6N2E2_4713at 5449.694 kb on + strand, within Pf6N2E2_4713at 5449.694 kb on + strand, within Pf6N2E2_4713at 5449.760 kb on + strand, within Pf6N2E2_4713at 5449.761 kb on - strand, within Pf6N2E2_4713at 5449.761 kb on - strand, within Pf6N2E2_4713at 5449.865 kb on - strand, within Pf6N2E2_4713at 5449.922 kb on + strand, within Pf6N2E2_4713at 5449.922 kb on + strand, within Pf6N2E2_4713at 5449.922 kb on + strand, within Pf6N2E2_4713at 5449.922 kb on + strand, within Pf6N2E2_4713at 5449.922 kb on + strand, within Pf6N2E2_4713at 5449.922 kb on + strand, within Pf6N2E2_4713at 5449.923 kb on - strand, within Pf6N2E2_4713at 5449.923 kb on - strand, within Pf6N2E2_4713at 5449.923 kb on - strand, within Pf6N2E2_4713at 5449.923 kb on - strand, within Pf6N2E2_4713at 5449.923 kb on - strand, within Pf6N2E2_4713at 5449.923 kb on - strand, within Pf6N2E2_4713at 5449.927 kb on - strand, within Pf6N2E2_4713at 5450.021 kb on + strandat 5450.022 kb on - strandat 5450.062 kb on + strandat 5450.118 kb on + strandat 5450.139 kb on + strandat 5450.139 kb on + strandat 5450.140 kb on - strandat 5450.155 kb on + strandat 5450.155 kb on + strandat 5450.155 kb on + strandat 5450.156 kb on - strandat 5450.323 kb on + strandat 5450.327 kb on + strandat 5450.327 kb on + strandat 5450.327 kb on + strandat 5450.327 kb on + strandat 5450.327 kb on + strandat 5450.327 kb on + strandat 5450.327 kb on + strandat 5450.327 kb on + strandat 5450.327 kb on + strandat 5450.328 kb on - strandat 5450.328 kb on - strandat 5450.328 kb on - strandat 5450.328 kb on - strandat 5450.328 kb on - strandat 5450.328 kb on - strandat 5450.344 kb on + strandat 5450.348 kb on + strandat 5450.348 kb on + strandat 5450.349 kb on - strandat 5450.349 kb on - strandat 5450.349 kb on - strandat 5450.426 kb on - strandat 5450.473 kb on + strand, within Pf6N2E2_4714at 5450.473 kb on + strand, within Pf6N2E2_4714at 5450.473 kb on + strand, within Pf6N2E2_4714at 5450.474 kb on - strand, within Pf6N2E2_4714at 5450.474 kb on - strand, within Pf6N2E2_4714at 5450.474 kb on - strand, within Pf6N2E2_4714at 5450.474 kb on - strand, within Pf6N2E2_4714at 5450.474 kb on - strand, within Pf6N2E2_4714at 5450.478 kb on + strand, within Pf6N2E2_4714at 5450.478 kb on + strand, within Pf6N2E2_4714at 5450.478 kb on + strand, within Pf6N2E2_4714at 5450.478 kb on + strand, within Pf6N2E2_4714at 5450.478 kb on + strand, within Pf6N2E2_4714at 5450.478 kb on + strand, within Pf6N2E2_4714at 5450.478 kb on + strand, within Pf6N2E2_4714at 5450.478 kb on + strand, within Pf6N2E2_4714at 5450.478 kb on + strand, within Pf6N2E2_4714at 5450.478 kb on + strand, within Pf6N2E2_4714at 5450.479 kb on - strand, within Pf6N2E2_4714at 5450.479 kb on - strand, within Pf6N2E2_4714at 5450.621 kb on + strand, within Pf6N2E2_4714at 5450.651 kb on + strand, within Pf6N2E2_4714at 5450.798 kb on + strand, within Pf6N2E2_4714at 5450.798 kb on + strand, within Pf6N2E2_4714at 5450.798 kb on + strand, within Pf6N2E2_4714at 5450.852 kb on + strand, within Pf6N2E2_4714at 5450.852 kb on + strand, within Pf6N2E2_4714at 5450.853 kb on - strand, within Pf6N2E2_4714at 5450.884 kb on + strand, within Pf6N2E2_4714at 5450.885 kb on - strand, within Pf6N2E2_4714at 5450.918 kb on + strand, within Pf6N2E2_4714at 5450.918 kb on + strand, within Pf6N2E2_4714at 5450.918 kb on + strand, within Pf6N2E2_4714at 5450.919 kb on - strand, within Pf6N2E2_4714at 5450.919 kb on - strand, within Pf6N2E2_4714at 5450.919 kb on - strand, within Pf6N2E2_4714at 5451.101 kb on + strand, within Pf6N2E2_4714at 5451.101 kb on + strand, within Pf6N2E2_4714at 5451.101 kb on + strand, within Pf6N2E2_4714at 5451.101 kb on + strand, within Pf6N2E2_4714at 5451.101 kb on + strand, within Pf6N2E2_4714at 5451.101 kb on + strand, within Pf6N2E2_4714at 5451.101 kb on + strand, within Pf6N2E2_4714at 5451.101 kb on + strand, within Pf6N2E2_4714at 5451.101 kb on + strand, within Pf6N2E2_4714at 5451.101 kb on + strand, within Pf6N2E2_4714at 5451.102 kb on - strand, within Pf6N2E2_4714at 5451.102 kb on - strand, within Pf6N2E2_4714at 5451.102 kb on - strand, within Pf6N2E2_4714at 5451.102 kb on - strand, within Pf6N2E2_4714at 5451.102 kb on - strand, within Pf6N2E2_4714at 5451.102 kb on - strand, within Pf6N2E2_4714at 5451.179 kb on - strand, within Pf6N2E2_4714at 5451.464 kb on + strandat 5451.465 kb on - strandat 5451.568 kb on + strandat 5451.673 kb on - strandat 5451.714 kb on - strandat 5451.778 kb on - strand, within Pf6N2E2_4715at 5451.778 kb on - strand, within Pf6N2E2_4715at 5451.778 kb on - strand, within Pf6N2E2_4715at 5452.027 kb on - strand, within Pf6N2E2_4715at 5452.258 kb on - strand, within Pf6N2E2_4715at 5452.258 kb on - strand, within Pf6N2E2_4715at 5452.476 kb on + strand, within Pf6N2E2_4715

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Per-strain Table

Position Strand Gene LocusTag Fraction Nickel (II) chloride 1 mM
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5,449,487 + Pf6N2E2_4713 0.50 +0.5
5,449,487 + Pf6N2E2_4713 0.50 +0.4
5,449,488 - Pf6N2E2_4713 0.50 -0.6
5,449,577 + Pf6N2E2_4713 0.57 -0.3
5,449,578 - Pf6N2E2_4713 0.57 +0.4
5,449,589 + Pf6N2E2_4713 0.58 +0.0
5,449,589 + Pf6N2E2_4713 0.58 +0.1
5,449,590 - Pf6N2E2_4713 0.58 -0.1
5,449,590 - Pf6N2E2_4713 0.58 +1.4
5,449,590 - Pf6N2E2_4713 0.58 -0.4
5,449,590 - Pf6N2E2_4713 0.58 -1.0
5,449,590 - Pf6N2E2_4713 0.58 +0.3
5,449,655 + Pf6N2E2_4713 0.63 -0.0
5,449,694 + Pf6N2E2_4713 0.66 +0.0
5,449,694 + Pf6N2E2_4713 0.66 +0.4
5,449,760 + Pf6N2E2_4713 0.71 +1.2
5,449,761 - Pf6N2E2_4713 0.71 +0.3
5,449,761 - Pf6N2E2_4713 0.71 +0.1
5,449,865 - Pf6N2E2_4713 0.79 -0.8
5,449,922 + Pf6N2E2_4713 0.84 -2.2
5,449,922 + Pf6N2E2_4713 0.84 -0.3
5,449,922 + Pf6N2E2_4713 0.84 +1.1
5,449,922 + Pf6N2E2_4713 0.84 +0.4
5,449,922 + Pf6N2E2_4713 0.84 +0.4
5,449,922 + Pf6N2E2_4713 0.84 +0.8
5,449,923 - Pf6N2E2_4713 0.84 -0.3
5,449,923 - Pf6N2E2_4713 0.84 +0.0
5,449,923 - Pf6N2E2_4713 0.84 +0.5
5,449,923 - Pf6N2E2_4713 0.84 -0.6
5,449,923 - Pf6N2E2_4713 0.84 -0.6
5,449,923 - Pf6N2E2_4713 0.84 +0.4
5,449,927 - Pf6N2E2_4713 0.84 +1.0
5,450,021 + +0.2
5,450,022 - +1.1
5,450,062 + +0.0
5,450,118 + -0.2
5,450,139 + +0.4
5,450,139 + +0.3
5,450,140 - +0.2
5,450,155 + -0.6
5,450,155 + -0.4
5,450,155 + -0.2
5,450,156 - -0.2
5,450,323 + +1.0
5,450,327 + +0.5
5,450,327 + +0.7
5,450,327 + -0.7
5,450,327 + -0.7
5,450,327 + -1.2
5,450,327 + +0.7
5,450,327 + -0.4
5,450,327 + -0.2
5,450,327 + +0.2
5,450,328 - -0.4
5,450,328 - +0.8
5,450,328 - -0.5
5,450,328 - -0.6
5,450,328 - -0.8
5,450,328 - -1.1
5,450,344 + -1.5
5,450,348 + -0.5
5,450,348 + -0.6
5,450,349 - -0.5
5,450,349 - -0.1
5,450,349 - -0.4
5,450,426 - +0.3
5,450,473 + Pf6N2E2_4714 0.10 -0.6
5,450,473 + Pf6N2E2_4714 0.10 +1.4
5,450,473 + Pf6N2E2_4714 0.10 -0.2
5,450,474 - Pf6N2E2_4714 0.11 -0.2
5,450,474 - Pf6N2E2_4714 0.11 +0.3
5,450,474 - Pf6N2E2_4714 0.11 -0.2
5,450,474 - Pf6N2E2_4714 0.11 +0.8
5,450,474 - Pf6N2E2_4714 0.11 +0.4
5,450,478 + Pf6N2E2_4714 0.11 +0.4
5,450,478 + Pf6N2E2_4714 0.11 -1.5
5,450,478 + Pf6N2E2_4714 0.11 +0.0
5,450,478 + Pf6N2E2_4714 0.11 +0.4
5,450,478 + Pf6N2E2_4714 0.11 +0.9
5,450,478 + Pf6N2E2_4714 0.11 -0.2
5,450,478 + Pf6N2E2_4714 0.11 +0.3
5,450,478 + Pf6N2E2_4714 0.11 +1.4
5,450,478 + Pf6N2E2_4714 0.11 -0.5
5,450,478 + Pf6N2E2_4714 0.11 -0.4
5,450,479 - Pf6N2E2_4714 0.11 -0.9
5,450,479 - Pf6N2E2_4714 0.11 +0.9
5,450,621 + Pf6N2E2_4714 0.23 -0.1
5,450,651 + Pf6N2E2_4714 0.26 +0.4
5,450,798 + Pf6N2E2_4714 0.38 +0.7
5,450,798 + Pf6N2E2_4714 0.38 -1.2
5,450,798 + Pf6N2E2_4714 0.38 -0.5
5,450,852 + Pf6N2E2_4714 0.43 +0.3
5,450,852 + Pf6N2E2_4714 0.43 +0.2
5,450,853 - Pf6N2E2_4714 0.43 +0.2
5,450,884 + Pf6N2E2_4714 0.46 +0.2
5,450,885 - Pf6N2E2_4714 0.46 +0.1
5,450,918 + Pf6N2E2_4714 0.49 +0.1
5,450,918 + Pf6N2E2_4714 0.49 +0.6
5,450,918 + Pf6N2E2_4714 0.49 -1.0
5,450,919 - Pf6N2E2_4714 0.49 -0.2
5,450,919 - Pf6N2E2_4714 0.49 +0.9
5,450,919 - Pf6N2E2_4714 0.49 -0.1
5,451,101 + Pf6N2E2_4714 0.64 +0.4
5,451,101 + Pf6N2E2_4714 0.64 +0.1
5,451,101 + Pf6N2E2_4714 0.64 -0.1
5,451,101 + Pf6N2E2_4714 0.64 -0.5
5,451,101 + Pf6N2E2_4714 0.64 -0.4
5,451,101 + Pf6N2E2_4714 0.64 +0.1
5,451,101 + Pf6N2E2_4714 0.64 -1.6
5,451,101 + Pf6N2E2_4714 0.64 -0.4
5,451,101 + Pf6N2E2_4714 0.64 -0.3
5,451,101 + Pf6N2E2_4714 0.64 -1.1
5,451,102 - Pf6N2E2_4714 0.64 +0.4
5,451,102 - Pf6N2E2_4714 0.64 -0.2
5,451,102 - Pf6N2E2_4714 0.64 -1.0
5,451,102 - Pf6N2E2_4714 0.64 +0.7
5,451,102 - Pf6N2E2_4714 0.64 -0.1
5,451,102 - Pf6N2E2_4714 0.64 +0.0
5,451,179 - Pf6N2E2_4714 0.71 +0.6
5,451,464 + -0.7
5,451,465 - +0.3
5,451,568 + +1.2
5,451,673 - -1.1
5,451,714 - -2.0
5,451,778 - Pf6N2E2_4715 0.10 +0.4
5,451,778 - Pf6N2E2_4715 0.10 +0.4
5,451,778 - Pf6N2E2_4715 0.10 -0.1
5,452,027 - Pf6N2E2_4715 0.34 -1.0
5,452,258 - Pf6N2E2_4715 0.56 +1.2
5,452,258 - Pf6N2E2_4715 0.56 -0.2
5,452,476 + Pf6N2E2_4715 0.77 +0.2

Or see this region's nucleotide sequence