Experiment: Fungal_growth_media_mCAFEs_v1 0.5x with Mixed culture; Trichoderma harzianum M10 spores at 2.5*10^5 spores/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS09615 and BWI76_RS09620 are separated by 138 nucleotides BWI76_RS09620 and BWI76_RS09625 overlap by 73 nucleotides BWI76_RS09625 and BWI76_RS09630 are separated by 0 nucleotides
BWI76_RS09615: BWI76_RS09615 - ATP-dependent Clp protease ATP-binding subunit ClpA, at 2,011,511 to 2,013,793
_RS09615
BWI76_RS09620: BWI76_RS09620 - translation initiation factor IF-1, at 2,013,932 to 2,014,150
_RS09620
BWI76_RS09625: BWI76_RS09625 - hypothetical protein, at 2,014,078 to 2,014,429
_RS09625
BWI76_RS09630: BWI76_RS09630 - leucyl/phenylalanyl-tRNA--protein transferase, at 2,014,430 to 2,015,134
_RS09630
Position (kb)
2013
2014
2015 Strain fitness (log2 ratio)
-1
0
1 at 2013.343 kb on + strand, within BWI76_RS09615 at 2013.483 kb on - strand, within BWI76_RS09615 at 2014.300 kb on + strand, within BWI76_RS09625 at 2014.875 kb on - strand, within BWI76_RS09630 at 2014.886 kb on - strand, within BWI76_RS09630 at 2015.107 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Fungal_growth_media_mCAFEs_v1 0.5x with Mixed culture; Trichoderma harzianum M10 spores at 2.5*10^5 spores/mL remove 2,013,343 + BWI76_RS09615 0.80 +0.9 2,013,483 - BWI76_RS09615 0.86 -0.6 2,014,300 + BWI76_RS09625 0.63 -0.7 2,014,875 - BWI76_RS09630 0.63 -1.1 2,014,886 - BWI76_RS09630 0.65 -0.1 2,015,107 + +0.4
Or see this region's nucleotide sequence