Experiment: Fungal_growth_media_mCAFEs_v1 0.5x with Mixed culture; Trichoderma harzianum M10 spores at 2.5*10^5 spores/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS07905 and BWI76_RS07910 are separated by 157 nucleotides BWI76_RS07910 and BWI76_RS07915 overlap by 4 nucleotides BWI76_RS07915 and BWI76_RS07920 are separated by 173 nucleotides
BWI76_RS07905: BWI76_RS07905 - trimethylamine-N-oxide reductase (cytochrome c), at 1,678,470 to 1,680,779
_RS07905
BWI76_RS07910: BWI76_RS07910 - acireductone dioxygenase, at 1,680,937 to 1,681,479
_RS07910
BWI76_RS07915: BWI76_RS07915 - acireductone synthase, at 1,681,476 to 1,682,165
_RS07915
BWI76_RS07920: BWI76_RS07920 - methionine aminotransferase, at 1,682,339 to 1,683,499
_RS07920
Position (kb) 1681
1682
1683 Strain fitness (log2 ratio) -1
0
1 at 1681.480 kb on + strand at 1681.705 kb on - strand, within BWI76_RS07915 at 1681.779 kb on + strand, within BWI76_RS07915 at 1681.899 kb on + strand, within BWI76_RS07915 at 1681.906 kb on - strand, within BWI76_RS07915 at 1682.022 kb on - strand, within BWI76_RS07915 at 1682.089 kb on + strand, within BWI76_RS07915 at 1682.311 kb on - strand at 1682.737 kb on + strand, within BWI76_RS07920 at 1682.836 kb on + strand, within BWI76_RS07920 at 1682.842 kb on + strand, within BWI76_RS07920 at 1682.978 kb on + strand, within BWI76_RS07920 at 1683.133 kb on + strand, within BWI76_RS07920
Per-strain Table
Position Strand Gene LocusTag Fraction Fungal_growth_media_mCAFEs_v1 0.5x with Mixed culture; Trichoderma harzianum M10 spores at 2.5*10^5 spores/mL remove 1,681,480 + -1.3 1,681,705 - BWI76_RS07915 0.33 -0.1 1,681,779 + BWI76_RS07915 0.44 -1.1 1,681,899 + BWI76_RS07915 0.61 -1.1 1,681,906 - BWI76_RS07915 0.62 -0.6 1,682,022 - BWI76_RS07915 0.79 -0.8 1,682,089 + BWI76_RS07915 0.89 -1.4 1,682,311 - +1.6 1,682,737 + BWI76_RS07920 0.34 -0.5 1,682,836 + BWI76_RS07920 0.43 +1.4 1,682,842 + BWI76_RS07920 0.43 +0.3 1,682,978 + BWI76_RS07920 0.55 +1.0 1,683,133 + BWI76_RS07920 0.68 +0.6
Or see this region's nucleotide sequence