Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS06550

Experiment: Fungal_growth_media_mCAFEs_v1 0.5x with Mixed culture; Trichoderma harzianum M10 spores at 2.5*10^5 spores/mL

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS06545 and BWI76_RS06550 are separated by 148 nucleotidesBWI76_RS06550 and BWI76_RS06555 are separated by 110 nucleotidesBWI76_RS06555 and BWI76_RS06560 are separated by 201 nucleotides BWI76_RS06545: BWI76_RS06545 - peptidylprolyl isomerase, at 1,375,750 to 1,377,624 _RS06545 BWI76_RS06550: BWI76_RS06550 - competence protein ComEA, at 1,377,773 to 1,378,138 _RS06550 BWI76_RS06555: BWI76_RS06555 - thioesterase superfamily protein, at 1,378,249 to 1,378,650 _RS06555 BWI76_RS06560: BWI76_RS06560 - 7-cyano-7-deazaguanine synthase QueC, at 1,378,852 to 1,379,547 _RS06560 Position (kb) 1377 1378 1379Strain fitness (log2 ratio) -3 -2 -1 0 1 2 3at 1376.829 kb on + strandat 1377.238 kb on - strand, within BWI76_RS06545at 1377.660 kb on - strandat 1377.806 kb on + strandat 1377.819 kb on - strand, within BWI76_RS06550at 1377.918 kb on + strand, within BWI76_RS06550at 1378.025 kb on + strand, within BWI76_RS06550at 1378.079 kb on - strand, within BWI76_RS06550at 1378.745 kb on + strandat 1378.987 kb on + strand, within BWI76_RS06560at 1378.992 kb on + strand, within BWI76_RS06560at 1379.119 kb on + strand, within BWI76_RS06560

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Per-strain Table

Position Strand Gene LocusTag Fraction Fungal_growth_media_mCAFEs_v1 0.5x with Mixed culture; Trichoderma harzianum M10 spores at 2.5*10^5 spores/mL
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1,376,829 + +3.2
1,377,238 - BWI76_RS06545 0.79 -1.5
1,377,660 - -0.5
1,377,806 + +0.8
1,377,819 - BWI76_RS06550 0.13 -0.2
1,377,918 + BWI76_RS06550 0.40 -1.3
1,378,025 + BWI76_RS06550 0.69 +0.4
1,378,079 - BWI76_RS06550 0.84 +1.1
1,378,745 + +0.8
1,378,987 + BWI76_RS06560 0.19 +0.7
1,378,992 + BWI76_RS06560 0.20 +1.0
1,379,119 + BWI76_RS06560 0.38 -3.1

Or see this region's nucleotide sequence