Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS22055

Experiment: Fungal_growth_media_mCAFEs_v1 0.5x with Mixed culture; Trichoderma harzianum M10 spores at 2.5*10^6 spores/mL

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS22040 and BWI76_RS22045 are separated by 183 nucleotidesBWI76_RS22045 and BWI76_RS22050 overlap by 4 nucleotidesBWI76_RS22050 and BWI76_RS22055 overlap by 28 nucleotidesBWI76_RS22055 and BWI76_RS22060 are separated by 10 nucleotides BWI76_RS22040: BWI76_RS22040 - carboxymuconolactone decarboxylase family protein, at 4,601,727 to 4,602,158 _RS22040 BWI76_RS22045: BWI76_RS22045 - NrdH-redoxin, at 4,602,342 to 4,602,587 _RS22045 BWI76_RS22050: BWI76_RS22050 - class Ib ribonucleoside-diphosphate reductase assembly flavoprotein NrdI, at 4,602,584 to 4,602,994 _RS22050 BWI76_RS22055: BWI76_RS22055 - ribonucleotide-diphosphate reductase, at 4,602,967 to 4,605,111 _RS22055 BWI76_RS22060: BWI76_RS22060 - ribonucleotide-diphosphate reductase subunit beta, at 4,605,122 to 4,606,084 _RS22060 Position (kb) 4602 4603 4604 4605 4606Strain fitness (log2 ratio) -4 -3 -2 -1 0 1 2 3 4at 4602.856 kb on - strand, within BWI76_RS22050at 4602.916 kb on + strand, within BWI76_RS22050at 4602.924 kb on + strand, within BWI76_RS22050at 4602.924 kb on + strand, within BWI76_RS22050at 4602.988 kb on - strandat 4603.129 kb on + strandat 4603.374 kb on - strand, within BWI76_RS22055at 4603.846 kb on + strand, within BWI76_RS22055at 4603.861 kb on - strand, within BWI76_RS22055at 4603.871 kb on + strand, within BWI76_RS22055at 4603.898 kb on - strand, within BWI76_RS22055at 4604.163 kb on - strand, within BWI76_RS22055at 4604.187 kb on - strand, within BWI76_RS22055at 4604.194 kb on + strand, within BWI76_RS22055at 4604.321 kb on + strand, within BWI76_RS22055at 4604.363 kb on + strand, within BWI76_RS22055at 4604.377 kb on - strand, within BWI76_RS22055at 4604.377 kb on - strand, within BWI76_RS22055at 4604.393 kb on + strand, within BWI76_RS22055at 4604.484 kb on + strand, within BWI76_RS22055at 4604.620 kb on + strand, within BWI76_RS22055at 4604.620 kb on + strand, within BWI76_RS22055at 4604.823 kb on + strand, within BWI76_RS22055at 4604.868 kb on + strand, within BWI76_RS22055at 4604.904 kb on + strandat 4604.949 kb on + strandat 4604.959 kb on - strandat 4605.039 kb on + strandat 4605.099 kb on - strandat 4605.139 kb on + strandat 4605.162 kb on + strandat 4605.263 kb on + strand, within BWI76_RS22060at 4605.264 kb on + strand, within BWI76_RS22060at 4605.309 kb on + strand, within BWI76_RS22060at 4605.377 kb on - strand, within BWI76_RS22060at 4605.377 kb on - strand, within BWI76_RS22060at 4605.377 kb on - strand, within BWI76_RS22060at 4605.397 kb on + strand, within BWI76_RS22060at 4605.430 kb on - strand, within BWI76_RS22060at 4605.539 kb on - strand, within BWI76_RS22060at 4605.704 kb on + strand, within BWI76_RS22060at 4605.704 kb on + strand, within BWI76_RS22060at 4605.751 kb on + strand, within BWI76_RS22060at 4605.756 kb on + strand, within BWI76_RS22060at 4605.765 kb on + strand, within BWI76_RS22060at 4605.806 kb on + strand, within BWI76_RS22060at 4605.826 kb on + strand, within BWI76_RS22060at 4605.834 kb on + strand, within BWI76_RS22060at 4605.834 kb on - strand, within BWI76_RS22060at 4605.842 kb on - strand, within BWI76_RS22060at 4605.842 kb on - strand, within BWI76_RS22060at 4605.842 kb on - strand, within BWI76_RS22060at 4605.861 kb on + strand, within BWI76_RS22060at 4605.864 kb on - strand, within BWI76_RS22060at 4605.869 kb on - strand, within BWI76_RS22060at 4605.903 kb on - strand, within BWI76_RS22060at 4605.968 kb on - strand, within BWI76_RS22060at 4606.033 kb on - strandat 4606.047 kb on - strandat 4606.068 kb on - strandat 4606.078 kb on + strandat 4606.078 kb on + strandat 4606.084 kb on + strandat 4606.100 kb on + strandat 4606.108 kb on - strandat 4606.110 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Fungal_growth_media_mCAFEs_v1 0.5x with Mixed culture; Trichoderma harzianum M10 spores at 2.5*10^6 spores/mL
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4,602,856 - BWI76_RS22050 0.66 -1.2
4,602,916 + BWI76_RS22050 0.81 -1.5
4,602,924 + BWI76_RS22050 0.83 +0.4
4,602,924 + BWI76_RS22050 0.83 -1.7
4,602,988 - -0.2
4,603,129 + +0.5
4,603,374 - BWI76_RS22055 0.19 +0.1
4,603,846 + BWI76_RS22055 0.41 -2.7
4,603,861 - BWI76_RS22055 0.42 -2.4
4,603,871 + BWI76_RS22055 0.42 -0.0
4,603,898 - BWI76_RS22055 0.43 +2.8
4,604,163 - BWI76_RS22055 0.56 -0.2
4,604,187 - BWI76_RS22055 0.57 +0.4
4,604,194 + BWI76_RS22055 0.57 +2.1
4,604,321 + BWI76_RS22055 0.63 +0.5
4,604,363 + BWI76_RS22055 0.65 +1.4
4,604,377 - BWI76_RS22055 0.66 -0.1
4,604,377 - BWI76_RS22055 0.66 -0.1
4,604,393 + BWI76_RS22055 0.66 +0.2
4,604,484 + BWI76_RS22055 0.71 +0.4
4,604,620 + BWI76_RS22055 0.77 -2.6
4,604,620 + BWI76_RS22055 0.77 +0.2
4,604,823 + BWI76_RS22055 0.87 -1.2
4,604,868 + BWI76_RS22055 0.89 -0.6
4,604,904 + +0.3
4,604,949 + -2.0
4,604,959 - +0.3
4,605,039 + +1.0
4,605,099 - +0.7
4,605,139 + +0.5
4,605,162 + -1.8
4,605,263 + BWI76_RS22060 0.15 -0.7
4,605,264 + BWI76_RS22060 0.15 -0.0
4,605,309 + BWI76_RS22060 0.19 -0.3
4,605,377 - BWI76_RS22060 0.26 -0.3
4,605,377 - BWI76_RS22060 0.26 -0.4
4,605,377 - BWI76_RS22060 0.26 +2.1
4,605,397 + BWI76_RS22060 0.29 +0.8
4,605,430 - BWI76_RS22060 0.32 +0.3
4,605,539 - BWI76_RS22060 0.43 -1.0
4,605,704 + BWI76_RS22060 0.60 -0.5
4,605,704 + BWI76_RS22060 0.60 +0.3
4,605,751 + BWI76_RS22060 0.65 +0.4
4,605,756 + BWI76_RS22060 0.66 +3.9
4,605,765 + BWI76_RS22060 0.67 +2.6
4,605,806 + BWI76_RS22060 0.71 +1.1
4,605,826 + BWI76_RS22060 0.73 -0.3
4,605,834 + BWI76_RS22060 0.74 +0.0
4,605,834 - BWI76_RS22060 0.74 -0.4
4,605,842 - BWI76_RS22060 0.75 +1.2
4,605,842 - BWI76_RS22060 0.75 -0.5
4,605,842 - BWI76_RS22060 0.75 -0.2
4,605,861 + BWI76_RS22060 0.77 +1.0
4,605,864 - BWI76_RS22060 0.77 -2.2
4,605,869 - BWI76_RS22060 0.78 +0.4
4,605,903 - BWI76_RS22060 0.81 +1.7
4,605,968 - BWI76_RS22060 0.88 +0.2
4,606,033 - +0.7
4,606,047 - +0.3
4,606,068 - +0.2
4,606,078 + +0.5
4,606,078 + -0.0
4,606,084 + +0.4
4,606,100 + +1.0
4,606,108 - +1.5
4,606,110 + -3.9

Or see this region's nucleotide sequence