Experiment: Fungal_growth_media_mCAFEs_v1 0.5x with Mixed culture; Trichoderma harzianum M10 spores at 2.5*10^6 spores/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS21440 and BWI76_RS21445 are separated by 127 nucleotides BWI76_RS21445 and BWI76_RS21450 overlap by 4 nucleotides BWI76_RS21450 and BWI76_RS21455 are separated by 132 nucleotides
BWI76_RS21440: BWI76_RS21440 - ATP-dependent chaperone ClpB, at 4,490,443 to 4,493,016
_RS21440
BWI76_RS21445: BWI76_RS21445 - hypothetical protein, at 4,493,144 to 4,493,875
_RS21445
BWI76_RS21450: BWI76_RS21450 - 23S rRNA pseudouridine(1911/1915/1917) synthase, at 4,493,872 to 4,494,852
_RS21450
BWI76_RS21455: BWI76_RS21455 - outer membrane protein assembly factor BamD, at 4,494,985 to 4,495,722
_RS21455
Position (kb) 4493
4494
4495 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 4492.920 kb on - strand at 4492.973 kb on - strand at 4492.985 kb on - strand at 4493.082 kb on + strand at 4493.090 kb on + strand at 4493.090 kb on + strand at 4493.098 kb on - strand at 4493.142 kb on + strand at 4493.156 kb on + strand at 4493.232 kb on - strand, within BWI76_RS21445 at 4493.386 kb on + strand, within BWI76_RS21445 at 4493.413 kb on + strand, within BWI76_RS21445 at 4494.068 kb on + strand, within BWI76_RS21450 at 4494.244 kb on + strand, within BWI76_RS21450 at 4494.284 kb on - strand, within BWI76_RS21450 at 4494.452 kb on + strand, within BWI76_RS21450 at 4494.835 kb on - strand at 4494.840 kb on - strand at 4494.842 kb on - strand at 4494.888 kb on - strand at 4495.793 kb on - strand at 4495.796 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Fungal_growth_media_mCAFEs_v1 0.5x with Mixed culture; Trichoderma harzianum M10 spores at 2.5*10^6 spores/mL remove 4,492,920 - +0.5 4,492,973 - -0.8 4,492,985 - -0.8 4,493,082 + -2.2 4,493,090 + +0.4 4,493,090 + -0.5 4,493,098 - +2.4 4,493,142 + +0.9 4,493,156 + -0.0 4,493,232 - BWI76_RS21445 0.12 +1.3 4,493,386 + BWI76_RS21445 0.33 -1.5 4,493,413 + BWI76_RS21445 0.37 -0.4 4,494,068 + BWI76_RS21450 0.20 -0.0 4,494,244 + BWI76_RS21450 0.38 -2.3 4,494,284 - BWI76_RS21450 0.42 -2.6 4,494,452 + BWI76_RS21450 0.59 +1.0 4,494,835 - -1.3 4,494,840 - -2.0 4,494,842 - -0.3 4,494,888 - -3.2 4,495,793 - +1.2 4,495,796 + -1.4
Or see this region's nucleotide sequence