Experiment: R2A with Vogels_fungal_media 0.8X
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HSERO_RS02085 and HSERO_RS02090 are separated by 113 nucleotides HSERO_RS02090 and HSERO_RS02095 are separated by 39 nucleotides HSERO_RS02095 and HSERO_RS02100 are separated by 179 nucleotides
HSERO_RS02085: HSERO_RS02085 - membrane protein, at 454,476 to 455,417
_RS02085
HSERO_RS02090: HSERO_RS02090 - Holliday junction DNA helicase RuvA, at 455,531 to 456,109
_RS02090
HSERO_RS02095: HSERO_RS02095 - Holliday junction DNA helicase RuvB, at 456,149 to 457,204
_RS02095
HSERO_RS02100: HSERO_RS02100 - 4-oxalocrotonate decarboxylase, at 457,384 to 458,226
_RS02100
Position (kb) 456
457
458 Strain fitness (log2 ratio) -2
-1
0
1 at 455.209 kb on + strand, within HSERO_RS02085 at 455.209 kb on + strand, within HSERO_RS02085 at 455.227 kb on + strand, within HSERO_RS02085 at 455.239 kb on + strand, within HSERO_RS02085 at 455.275 kb on + strand, within HSERO_RS02085 at 455.456 kb on - strand at 455.473 kb on + strand at 455.481 kb on - strand at 455.481 kb on - strand at 455.696 kb on + strand, within HSERO_RS02090 at 455.957 kb on + strand, within HSERO_RS02090 at 456.116 kb on + strand at 456.116 kb on + strand at 456.124 kb on - strand at 456.176 kb on + strand at 456.552 kb on - strand, within HSERO_RS02095 at 456.723 kb on - strand, within HSERO_RS02095 at 457.251 kb on - strand at 457.271 kb on - strand at 457.477 kb on - strand, within HSERO_RS02100 at 457.917 kb on + strand, within HSERO_RS02100
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Vogels_fungal_media 0.8X remove 455,209 + HSERO_RS02085 0.78 -0.1 455,209 + HSERO_RS02085 0.78 +0.1 455,227 + HSERO_RS02085 0.80 +0.1 455,239 + HSERO_RS02085 0.81 +0.1 455,275 + HSERO_RS02085 0.85 -0.8 455,456 - -0.0 455,473 + -0.4 455,481 - +0.1 455,481 - +0.5 455,696 + HSERO_RS02090 0.28 -1.8 455,957 + HSERO_RS02090 0.74 +1.0 456,116 + +0.2 456,116 + +0.1 456,124 - +0.5 456,176 + -0.4 456,552 - HSERO_RS02095 0.38 -0.4 456,723 - HSERO_RS02095 0.54 +0.5 457,251 - -0.3 457,271 - -0.2 457,477 - HSERO_RS02100 0.11 -1.1 457,917 + HSERO_RS02100 0.63 +1.1
Or see this region's nucleotide sequence