Experiment: R2A with Vogels_fungal_media 0.8X
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HSERO_RS01810 and HSERO_RS01815 are separated by 116 nucleotides HSERO_RS01815 and HSERO_RS01820 are separated by 87 nucleotides HSERO_RS01820 and HSERO_RS01825 are separated by 5 nucleotides
HSERO_RS01810: HSERO_RS01810 - GTPase CgtA, at 398,901 to 400,010
_RS01810
HSERO_RS01815: HSERO_RS01815 - gamma-glutamyl kinase, at 400,127 to 401,245
_RS01815
HSERO_RS01820: HSERO_RS01820 - hypothetical protein, at 401,333 to 401,911
_RS01820
HSERO_RS01825: HSERO_RS01825 - RNA pyrophosphohydrolase, at 401,917 to 402,501
_RS01825
Position (kb) 400
401
402 Strain fitness (log2 ratio) -2
-1
0
1
2 at 399.999 kb on + strand at 400.007 kb on - strand at 400.007 kb on - strand at 400.033 kb on - strand at 400.173 kb on + strand at 400.179 kb on - strand at 400.261 kb on + strand, within HSERO_RS01815 at 400.279 kb on + strand, within HSERO_RS01815 at 400.584 kb on + strand, within HSERO_RS01815 at 400.660 kb on + strand, within HSERO_RS01815 at 400.728 kb on + strand, within HSERO_RS01815 at 401.002 kb on - strand, within HSERO_RS01815 at 401.037 kb on - strand, within HSERO_RS01815 at 401.058 kb on + strand, within HSERO_RS01815 at 401.229 kb on + strand at 401.229 kb on + strand at 401.229 kb on + strand at 401.229 kb on + strand at 401.237 kb on - strand at 401.237 kb on - strand at 401.237 kb on - strand at 401.239 kb on + strand at 401.393 kb on - strand, within HSERO_RS01820 at 401.411 kb on + strand, within HSERO_RS01820 at 401.433 kb on + strand, within HSERO_RS01820 at 401.435 kb on + strand, within HSERO_RS01820 at 401.441 kb on - strand, within HSERO_RS01820 at 401.441 kb on - strand, within HSERO_RS01820 at 401.564 kb on + strand at 401.625 kb on - strand, within HSERO_RS01820 at 401.705 kb on - strand, within HSERO_RS01820 at 401.705 kb on - strand, within HSERO_RS01820 at 401.830 kb on - strand, within HSERO_RS01820 at 402.075 kb on - strand, within HSERO_RS01825 at 402.203 kb on + strand, within HSERO_RS01825
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Vogels_fungal_media 0.8X remove 399,999 + -0.8 400,007 - -0.7 400,007 - -0.4 400,033 - -0.1 400,173 + -1.3 400,179 - -0.8 400,261 + HSERO_RS01815 0.12 -0.7 400,279 + HSERO_RS01815 0.14 +1.1 400,584 + HSERO_RS01815 0.41 -0.2 400,660 + HSERO_RS01815 0.48 -1.0 400,728 + HSERO_RS01815 0.54 -1.8 401,002 - HSERO_RS01815 0.78 -0.0 401,037 - HSERO_RS01815 0.81 +0.1 401,058 + HSERO_RS01815 0.83 -0.4 401,229 + -0.1 401,229 + -0.2 401,229 + -0.1 401,229 + -1.7 401,237 - +0.6 401,237 - -0.1 401,237 - -0.3 401,239 + -0.3 401,393 - HSERO_RS01820 0.10 -0.4 401,411 + HSERO_RS01820 0.13 +0.2 401,433 + HSERO_RS01820 0.17 -0.2 401,435 + HSERO_RS01820 0.18 -0.5 401,441 - HSERO_RS01820 0.19 -0.3 401,441 - HSERO_RS01820 0.19 -0.1 401,564 + +2.1 401,625 - HSERO_RS01820 0.50 -0.0 401,705 - HSERO_RS01820 0.64 -0.0 401,705 - HSERO_RS01820 0.64 +0.4 401,830 - HSERO_RS01820 0.86 -0.3 402,075 - HSERO_RS01825 0.27 -0.2 402,203 + HSERO_RS01825 0.49 +0.9
Or see this region's nucleotide sequence