Experiment: R2A with Vogels_fungal_media 0.8X
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HSERO_RS01130 and HSERO_RS01135 overlap by 4 nucleotides HSERO_RS01135 and HSERO_RS01140 overlap by 4 nucleotides HSERO_RS01140 and HSERO_RS01145 are separated by 46 nucleotides HSERO_RS01145 and HSERO_RS01150 are separated by 362 nucleotides
HSERO_RS01130: HSERO_RS01130 - hypothetical protein, at 236,489 to 236,929
_RS01130
HSERO_RS01135: HSERO_RS01135 - hypothetical protein, at 236,926 to 237,348
_RS01135
HSERO_RS01140: HSERO_RS01140 - phage tail protein, at 237,345 to 237,704
_RS01140
HSERO_RS01145: HSERO_RS01145 - inner membrane protein, at 237,751 to 238,032
_RS01145
HSERO_RS01150: HSERO_RS01150 - membrane protein, at 238,395 to 239,696
_RS01150
Position (kb) 237
238
239 Strain fitness (log2 ratio) -1
0
1 at 236.811 kb on + strand, within HSERO_RS01130 at 236.819 kb on - strand at 236.828 kb on - strand, within HSERO_RS01130 at 236.990 kb on - strand, within HSERO_RS01135 at 236.990 kb on - strand, within HSERO_RS01135 at 237.026 kb on - strand, within HSERO_RS01135 at 237.092 kb on + strand at 237.101 kb on - strand, within HSERO_RS01135 at 237.173 kb on + strand, within HSERO_RS01135 at 237.173 kb on + strand, within HSERO_RS01135 at 237.196 kb on - strand, within HSERO_RS01135 at 237.210 kb on + strand, within HSERO_RS01135 at 237.218 kb on - strand, within HSERO_RS01135 at 237.502 kb on + strand, within HSERO_RS01140 at 237.510 kb on - strand, within HSERO_RS01140 at 237.650 kb on - strand, within HSERO_RS01140 at 237.688 kb on - strand at 237.876 kb on + strand at 237.997 kb on - strand, within HSERO_RS01145 at 238.094 kb on + strand at 238.111 kb on + strand at 238.228 kb on + strand at 238.244 kb on - strand at 238.311 kb on - strand at 238.358 kb on - strand at 238.389 kb on - strand at 238.401 kb on - strand at 238.443 kb on + strand at 238.627 kb on + strand, within HSERO_RS01150 at 238.736 kb on - strand, within HSERO_RS01150 at 238.766 kb on + strand, within HSERO_RS01150 at 238.882 kb on - strand, within HSERO_RS01150 at 238.991 kb on - strand, within HSERO_RS01150
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Vogels_fungal_media 0.8X remove 236,811 + HSERO_RS01130 0.73 +0.0 236,819 - +0.8 236,828 - HSERO_RS01130 0.77 +0.3 236,990 - HSERO_RS01135 0.15 +0.0 236,990 - HSERO_RS01135 0.15 +0.0 237,026 - HSERO_RS01135 0.24 +0.3 237,092 + +0.7 237,101 - HSERO_RS01135 0.41 -0.0 237,173 + HSERO_RS01135 0.58 -0.4 237,173 + HSERO_RS01135 0.58 -0.5 237,196 - HSERO_RS01135 0.64 -0.1 237,210 + HSERO_RS01135 0.67 +0.3 237,218 - HSERO_RS01135 0.69 +0.0 237,502 + HSERO_RS01140 0.44 -0.1 237,510 - HSERO_RS01140 0.46 +0.1 237,650 - HSERO_RS01140 0.85 +0.2 237,688 - -1.7 237,876 + +0.9 237,997 - HSERO_RS01145 0.87 -0.6 238,094 + +0.3 238,111 + +1.1 238,228 + -0.2 238,244 - -0.3 238,311 - -0.0 238,358 - +0.0 238,389 - +0.1 238,401 - -0.2 238,443 + -0.3 238,627 + HSERO_RS01150 0.18 +0.3 238,736 - HSERO_RS01150 0.26 +0.2 238,766 + HSERO_RS01150 0.28 +0.2 238,882 - HSERO_RS01150 0.37 -0.3 238,991 - HSERO_RS01150 0.46 +0.7
Or see this region's nucleotide sequence