Experiment: Fungal_growth_media_mCAFEs_v1 1x with Mixed culture; Trichoderma harzianum M10 spores at 2.5*10^4 spores/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS01530 and BWI76_RS01535 overlap by 4 nucleotides BWI76_RS01535 and BWI76_RS01540 are separated by 1 nucleotides BWI76_RS01540 and BWI76_RS01545 overlap by 17 nucleotides BWI76_RS01545 and BWI76_RS01550 overlap by 8 nucleotides
BWI76_RS01530: BWI76_RS01530 - 2-iminoacetate synthase ThiH, at 312,554 to 313,687
_RS01530
BWI76_RS01535: BWI76_RS01535 - thiazole synthase, at 313,684 to 314,454
_RS01535
BWI76_RS01540: BWI76_RS01540 - thiamine biosynthesis protein ThiS, at 314,456 to 314,656
_RS01540
BWI76_RS01545: BWI76_RS01545 - thiamine biosynthesis protein ThiS, at 314,640 to 315,395
_RS01545
BWI76_RS01550: BWI76_RS01550 - thiamine phosphate synthase, at 315,388 to 316,023
_RS01550
Position (kb) 313
314
315 Strain fitness (log2 ratio) -2
-1
0
1 at 312.941 kb on + strand, within BWI76_RS01530 at 313.038 kb on + strand, within BWI76_RS01530 at 313.466 kb on + strand, within BWI76_RS01530 at 313.636 kb on + strand at 313.636 kb on + strand at 313.716 kb on + strand at 313.846 kb on + strand at 313.849 kb on - strand, within BWI76_RS01535 at 313.908 kb on - strand, within BWI76_RS01535 at 314.057 kb on - strand, within BWI76_RS01535 at 314.068 kb on - strand, within BWI76_RS01535 at 314.080 kb on + strand, within BWI76_RS01535 at 314.178 kb on + strand, within BWI76_RS01535 at 314.257 kb on - strand, within BWI76_RS01535 at 314.337 kb on + strand, within BWI76_RS01535 at 314.624 kb on + strand, within BWI76_RS01540 at 314.624 kb on + strand, within BWI76_RS01540 at 314.743 kb on + strand, within BWI76_RS01545 at 315.138 kb on - strand, within BWI76_RS01545 at 315.202 kb on - strand, within BWI76_RS01545 at 315.435 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Fungal_growth_media_mCAFEs_v1 1x with Mixed culture; Trichoderma harzianum M10 spores at 2.5*10^4 spores/mL remove 312,941 + BWI76_RS01530 0.34 -1.7 313,038 + BWI76_RS01530 0.43 -0.8 313,466 + BWI76_RS01530 0.80 -1.0 313,636 + -1.2 313,636 + -1.0 313,716 + -0.9 313,846 + -0.1 313,849 - BWI76_RS01535 0.21 -1.1 313,908 - BWI76_RS01535 0.29 -0.6 314,057 - BWI76_RS01535 0.48 +0.6 314,068 - BWI76_RS01535 0.50 -0.7 314,080 + BWI76_RS01535 0.51 -0.6 314,178 + BWI76_RS01535 0.64 -1.0 314,257 - BWI76_RS01535 0.74 -0.7 314,337 + BWI76_RS01535 0.85 -1.1 314,624 + BWI76_RS01540 0.84 -0.4 314,624 + BWI76_RS01540 0.84 -2.0 314,743 + BWI76_RS01545 0.14 -2.1 315,138 - BWI76_RS01545 0.66 -1.7 315,202 - BWI76_RS01545 0.74 -0.4 315,435 + -2.3
Or see this region's nucleotide sequence