Experiment: Fungal_growth_media_mCAFEs_v1 1x with Mixed culture; Trichoderma harzianum M10 spores at 2.5*10^5 spores/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS22225 and BWI76_RS22230 are separated by 17 nucleotides BWI76_RS22230 and BWI76_RS22235 are separated by 133 nucleotides BWI76_RS22235 and BWI76_RS22240 are separated by 68 nucleotides BWI76_RS22240 and BWI76_RS22245 overlap by 8 nucleotides
BWI76_RS22225: BWI76_RS22225 - PTS sorbitol transporter subunit IIA, at 4,633,675 to 4,634,037
_RS22225
BWI76_RS22230: BWI76_RS22230 - Sorbitol-6-phosphate 2-dehydrogenase (EC 1.1.1.140) <small>(from data)</small>, at 4,634,055 to 4,634,834
_RS22230
BWI76_RS22235: BWI76_RS22235 - transcriptional regulator GutM, at 4,634,968 to 4,635,327
_RS22235
BWI76_RS22240: BWI76_RS22240 - transcriptional regulator, at 4,635,396 to 4,636,169
_RS22240
BWI76_RS22245: BWI76_RS22245 - arabinose 5-phosphate isomerase GutQ, at 4,636,162 to 4,637,127
_RS22245
Position (kb) 4634
4635
4636 Strain fitness (log2 ratio) -1
0
1 at 4633.977 kb on - strand, within BWI76_RS22225 at 4634.085 kb on + strand at 4634.184 kb on + strand, within BWI76_RS22230 at 4634.454 kb on - strand, within BWI76_RS22230 at 4634.508 kb on + strand, within BWI76_RS22230 at 4634.581 kb on + strand, within BWI76_RS22230 at 4634.664 kb on - strand, within BWI76_RS22230 at 4634.679 kb on - strand, within BWI76_RS22230 at 4635.182 kb on - strand, within BWI76_RS22235 at 4635.467 kb on - strand at 4635.495 kb on - strand, within BWI76_RS22240 at 4635.583 kb on - strand, within BWI76_RS22240 at 4635.705 kb on + strand, within BWI76_RS22240 at 4635.964 kb on - strand, within BWI76_RS22240 at 4636.176 kb on + strand at 4636.196 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Fungal_growth_media_mCAFEs_v1 1x with Mixed culture; Trichoderma harzianum M10 spores at 2.5*10^5 spores/mL remove 4,633,977 - BWI76_RS22225 0.83 +0.8 4,634,085 + +0.3 4,634,184 + BWI76_RS22230 0.17 +0.5 4,634,454 - BWI76_RS22230 0.51 -1.3 4,634,508 + BWI76_RS22230 0.58 -0.2 4,634,581 + BWI76_RS22230 0.67 -0.2 4,634,664 - BWI76_RS22230 0.78 +1.2 4,634,679 - BWI76_RS22230 0.80 +0.5 4,635,182 - BWI76_RS22235 0.59 +0.4 4,635,467 - -1.1 4,635,495 - BWI76_RS22240 0.13 +0.3 4,635,583 - BWI76_RS22240 0.24 +0.5 4,635,705 + BWI76_RS22240 0.40 +0.3 4,635,964 - BWI76_RS22240 0.73 +0.2 4,636,176 + -0.5 4,636,196 + -0.0
Or see this region's nucleotide sequence