Experiment: R2A with Vogels_fungal_media 0.8X
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HSERO_RS23180 and HSERO_RS23185 are separated by 65 nucleotides HSERO_RS23185 and HSERO_RS23190 are separated by 13 nucleotides HSERO_RS23190 and HSERO_RS23195 are separated by 271 nucleotides
HSERO_RS23180: HSERO_RS23180 - membrane protein, at 5,319,429 to 5,320,913
_RS23180
HSERO_RS23185: HSERO_RS23185 - succinyl-CoA:3-ketoacid-CoA transferase, at 5,320,979 to 5,321,629
_RS23185
HSERO_RS23190: HSERO_RS23190 - succinyl-CoA:3-ketoacid-CoA transferase, at 5,321,643 to 5,322,338
_RS23190
HSERO_RS23195: HSERO_RS23195 - LuxR family transcriptional regulator, at 5,322,610 to 5,323,170
_RS23195
Position (kb) 5320
5321
5322 Strain fitness (log2 ratio) -1
0
1 at 5320.204 kb on - strand, within HSERO_RS23180 at 5320.204 kb on - strand, within HSERO_RS23180 at 5320.249 kb on + strand, within HSERO_RS23180 at 5320.333 kb on - strand, within HSERO_RS23180 at 5320.362 kb on - strand, within HSERO_RS23180 at 5320.383 kb on + strand, within HSERO_RS23180 at 5320.411 kb on + strand, within HSERO_RS23180 at 5320.468 kb on + strand, within HSERO_RS23180 at 5320.505 kb on - strand, within HSERO_RS23180 at 5320.558 kb on + strand, within HSERO_RS23180 at 5320.580 kb on + strand, within HSERO_RS23180 at 5320.753 kb on + strand, within HSERO_RS23180 at 5320.854 kb on + strand at 5320.951 kb on - strand at 5320.989 kb on + strand at 5321.457 kb on + strand, within HSERO_RS23185 at 5322.445 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Vogels_fungal_media 0.8X remove 5,320,204 - HSERO_RS23180 0.52 +0.5 5,320,204 - HSERO_RS23180 0.52 +0.0 5,320,249 + HSERO_RS23180 0.55 +0.3 5,320,333 - HSERO_RS23180 0.61 +0.4 5,320,362 - HSERO_RS23180 0.63 -0.4 5,320,383 + HSERO_RS23180 0.64 +0.5 5,320,411 + HSERO_RS23180 0.66 -0.1 5,320,468 + HSERO_RS23180 0.70 -0.5 5,320,505 - HSERO_RS23180 0.72 -0.0 5,320,558 + HSERO_RS23180 0.76 +0.1 5,320,580 + HSERO_RS23180 0.78 +0.4 5,320,753 + HSERO_RS23180 0.89 +0.8 5,320,854 + +0.2 5,320,951 - +0.3 5,320,989 + +1.3 5,321,457 + HSERO_RS23185 0.73 -0.5 5,322,445 - -0.3
Or see this region's nucleotide sequence