Experiment: R2A with Vogels_fungal_media 0.8X
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HSERO_RS21075 and HSERO_RS21080 are separated by 22 nucleotides HSERO_RS21080 and HSERO_RS21085 are separated by 7 nucleotides
HSERO_RS21075: HSERO_RS21075 - asparagine synthase, at 4,820,788 to 4,822,671
_RS21075
HSERO_RS21080: HSERO_RS21080 - glycosyl transferase, at 4,822,694 to 4,823,896
_RS21080
HSERO_RS21085: HSERO_RS21085 - glycosyl transferase family 1, at 4,823,904 to 4,825,127
_RS21085
Position (kb)
4822
4823
4824 Strain fitness (log2 ratio)
-1
0
1 at 4821.711 kb on - strand, within HSERO_RS21075 at 4821.916 kb on - strand, within HSERO_RS21075 at 4821.991 kb on + strand, within HSERO_RS21075 at 4822.162 kb on + strand, within HSERO_RS21075 at 4822.225 kb on - strand, within HSERO_RS21075 at 4822.331 kb on + strand, within HSERO_RS21075 at 4822.514 kb on - strand at 4822.616 kb on - strand at 4822.626 kb on - strand at 4822.660 kb on + strand at 4822.668 kb on - strand at 4822.767 kb on - strand at 4822.866 kb on + strand, within HSERO_RS21080 at 4822.866 kb on + strand, within HSERO_RS21080 at 4822.876 kb on + strand, within HSERO_RS21080 at 4822.889 kb on - strand, within HSERO_RS21080 at 4822.993 kb on - strand, within HSERO_RS21080 at 4823.062 kb on + strand, within HSERO_RS21080 at 4823.270 kb on - strand, within HSERO_RS21080 at 4823.341 kb on + strand, within HSERO_RS21080 at 4823.544 kb on - strand, within HSERO_RS21080 at 4823.588 kb on - strand, within HSERO_RS21080 at 4823.671 kb on + strand, within HSERO_RS21080 at 4823.679 kb on - strand, within HSERO_RS21080 at 4823.795 kb on + strand at 4823.913 kb on - strand at 4824.030 kb on + strand, within HSERO_RS21085 at 4824.047 kb on + strand, within HSERO_RS21085 at 4824.053 kb on + strand, within HSERO_RS21085 at 4824.053 kb on - strand, within HSERO_RS21085 at 4824.072 kb on - strand, within HSERO_RS21085 at 4824.110 kb on + strand, within HSERO_RS21085 at 4824.144 kb on + strand, within HSERO_RS21085 at 4824.152 kb on - strand, within HSERO_RS21085 at 4824.152 kb on - strand, within HSERO_RS21085 at 4824.152 kb on - strand, within HSERO_RS21085 at 4824.280 kb on + strand, within HSERO_RS21085 at 4824.305 kb on - strand, within HSERO_RS21085 at 4824.317 kb on - strand at 4824.371 kb on + strand, within HSERO_RS21085 at 4824.617 kb on - strand, within HSERO_RS21085 at 4824.759 kb on + strand, within HSERO_RS21085
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Vogels_fungal_media 0.8X remove 4,821,711 - HSERO_RS21075 0.49 +1.5 4,821,916 - HSERO_RS21075 0.60 +0.3 4,821,991 + HSERO_RS21075 0.64 -0.2 4,822,162 + HSERO_RS21075 0.73 +0.9 4,822,225 - HSERO_RS21075 0.76 -0.7 4,822,331 + HSERO_RS21075 0.82 +0.0 4,822,514 - -1.2 4,822,616 - -0.4 4,822,626 - -0.2 4,822,660 + -0.4 4,822,668 - +0.4 4,822,767 - +0.1 4,822,866 + HSERO_RS21080 0.14 +0.9 4,822,866 + HSERO_RS21080 0.14 -0.8 4,822,876 + HSERO_RS21080 0.15 +0.1 4,822,889 - HSERO_RS21080 0.16 -1.0 4,822,993 - HSERO_RS21080 0.25 -0.4 4,823,062 + HSERO_RS21080 0.31 -0.8 4,823,270 - HSERO_RS21080 0.48 +0.2 4,823,341 + HSERO_RS21080 0.54 -0.8 4,823,544 - HSERO_RS21080 0.71 -0.6 4,823,588 - HSERO_RS21080 0.74 -0.3 4,823,671 + HSERO_RS21080 0.81 -0.6 4,823,679 - HSERO_RS21080 0.82 -1.1 4,823,795 + -0.0 4,823,913 - +0.6 4,824,030 + HSERO_RS21085 0.10 -0.1 4,824,047 + HSERO_RS21085 0.12 -0.0 4,824,053 + HSERO_RS21085 0.12 -0.4 4,824,053 - HSERO_RS21085 0.12 -0.1 4,824,072 - HSERO_RS21085 0.14 -0.3 4,824,110 + HSERO_RS21085 0.17 +0.3 4,824,144 + HSERO_RS21085 0.20 +0.1 4,824,152 - HSERO_RS21085 0.20 -0.7 4,824,152 - HSERO_RS21085 0.20 +0.2 4,824,152 - HSERO_RS21085 0.20 -0.5 4,824,280 + HSERO_RS21085 0.31 +1.0 4,824,305 - HSERO_RS21085 0.33 -0.3 4,824,317 - +0.5 4,824,371 + HSERO_RS21085 0.38 -0.3 4,824,617 - HSERO_RS21085 0.58 +0.2 4,824,759 + HSERO_RS21085 0.70 +0.5
Or see this region's nucleotide sequence