Experiment: R2A with Vogels_fungal_media 0.8X
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HSERO_RS20975 and HSERO_RS20980 are separated by 15 nucleotides HSERO_RS20980 and HSERO_RS20985 are separated by 3 nucleotides HSERO_RS20985 and HSERO_RS20990 are separated by 8 nucleotides
HSERO_RS20975: HSERO_RS20975 - UDP-galactose-4-epimerase, at 4,799,396 to 4,800,469
_RS20975
HSERO_RS20980: HSERO_RS20980 - oxidoreductase, at 4,800,485 to 4,801,543
_RS20980
HSERO_RS20985: HSERO_RS20985 - serine acetyltransferase, at 4,801,547 to 4,802,113
_RS20985
HSERO_RS20990: HSERO_RS20990 - aminotransferase DegT, at 4,802,122 to 4,803,210
_RS20990
Position (kb) 4800
4801
4802 Strain fitness (log2 ratio) -2
-1
0
1 at 4799.591 kb on + strand at 4799.649 kb on - strand, within HSERO_RS20975 at 4799.657 kb on + strand, within HSERO_RS20975 at 4799.657 kb on + strand, within HSERO_RS20975 at 4799.721 kb on + strand, within HSERO_RS20975 at 4799.754 kb on + strand, within HSERO_RS20975 at 4800.078 kb on - strand, within HSERO_RS20975 at 4800.093 kb on - strand, within HSERO_RS20975 at 4800.111 kb on - strand, within HSERO_RS20975 at 4800.205 kb on - strand, within HSERO_RS20975 at 4800.293 kb on + strand, within HSERO_RS20975 at 4800.326 kb on - strand, within HSERO_RS20975 at 4800.440 kb on - strand at 4800.509 kb on - strand at 4800.509 kb on - strand at 4800.606 kb on - strand, within HSERO_RS20980 at 4800.634 kb on + strand, within HSERO_RS20980 at 4800.855 kb on + strand, within HSERO_RS20980 at 4800.920 kb on - strand, within HSERO_RS20980 at 4801.125 kb on - strand, within HSERO_RS20980 at 4801.330 kb on - strand, within HSERO_RS20980 at 4801.381 kb on - strand, within HSERO_RS20980 at 4801.994 kb on - strand, within HSERO_RS20985 at 4802.150 kb on + strand at 4802.199 kb on - strand at 4802.294 kb on + strand, within HSERO_RS20990 at 4802.302 kb on - strand, within HSERO_RS20990 at 4802.362 kb on + strand, within HSERO_RS20990
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Vogels_fungal_media 0.8X remove 4,799,591 + +0.5 4,799,649 - HSERO_RS20975 0.24 -0.9 4,799,657 + HSERO_RS20975 0.24 +0.4 4,799,657 + HSERO_RS20975 0.24 -0.1 4,799,721 + HSERO_RS20975 0.30 -0.0 4,799,754 + HSERO_RS20975 0.33 -2.0 4,800,078 - HSERO_RS20975 0.64 +0.5 4,800,093 - HSERO_RS20975 0.65 +0.6 4,800,111 - HSERO_RS20975 0.67 +0.4 4,800,205 - HSERO_RS20975 0.75 -0.1 4,800,293 + HSERO_RS20975 0.84 -0.0 4,800,326 - HSERO_RS20975 0.87 +0.1 4,800,440 - -0.8 4,800,509 - -0.3 4,800,509 - +0.3 4,800,606 - HSERO_RS20980 0.11 +0.5 4,800,634 + HSERO_RS20980 0.14 -0.9 4,800,855 + HSERO_RS20980 0.35 -0.4 4,800,920 - HSERO_RS20980 0.41 -0.8 4,801,125 - HSERO_RS20980 0.60 -0.9 4,801,330 - HSERO_RS20980 0.80 -1.9 4,801,381 - HSERO_RS20980 0.85 -2.2 4,801,994 - HSERO_RS20985 0.79 -2.1 4,802,150 + -0.4 4,802,199 - -0.4 4,802,294 + HSERO_RS20990 0.16 -1.0 4,802,302 - HSERO_RS20990 0.17 -0.7 4,802,362 + HSERO_RS20990 0.22 -0.9
Or see this region's nucleotide sequence