Experiment: R2A with Vogels_fungal_media 0.8X
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HSERO_RS20615 and HSERO_RS20620 are separated by 13 nucleotides HSERO_RS20620 and HSERO_RS20625 are separated by 17 nucleotides HSERO_RS20625 and HSERO_RS20630 are separated by 63 nucleotides
HSERO_RS20615: HSERO_RS20615 - phenylacetate-CoA oxygenase subunit PaaB, at 4,728,335 to 4,728,619
_RS20615
HSERO_RS20620: HSERO_RS20620 - phenylacetic acid degradation protein, at 4,728,633 to 4,729,385
_RS20620
HSERO_RS20625: HSERO_RS20625 - phenylacetate-CoA oxygenase, at 4,729,403 to 4,729,927
_RS20625
HSERO_RS20630: HSERO_RS20630 - phenylacetic acid degradation protein, at 4,729,991 to 4,731,061
_RS20630
Position (kb) 4729
4730 Strain fitness (log2 ratio) -1
0
1 at 4728.516 kb on + strand, within HSERO_RS20615 at 4728.749 kb on - strand, within HSERO_RS20620 at 4728.880 kb on + strand, within HSERO_RS20620 at 4729.328 kb on - strand at 4729.379 kb on + strand at 4729.387 kb on - strand at 4729.609 kb on + strand, within HSERO_RS20625 at 4729.675 kb on + strand, within HSERO_RS20625 at 4730.024 kb on - strand at 4730.261 kb on + strand, within HSERO_RS20630 at 4730.425 kb on + strand, within HSERO_RS20630 at 4730.871 kb on + strand, within HSERO_RS20630
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Vogels_fungal_media 0.8X remove 4,728,516 + HSERO_RS20615 0.64 -0.2 4,728,749 - HSERO_RS20620 0.15 +1.1 4,728,880 + HSERO_RS20620 0.33 -1.1 4,729,328 - -0.1 4,729,379 + -1.0 4,729,387 - +0.4 4,729,609 + HSERO_RS20625 0.39 -0.3 4,729,675 + HSERO_RS20625 0.52 -0.6 4,730,024 - -0.6 4,730,261 + HSERO_RS20630 0.25 +0.6 4,730,425 + HSERO_RS20630 0.41 +0.7 4,730,871 + HSERO_RS20630 0.82 +0.9
Or see this region's nucleotide sequence