Experiment: R2A with Vogels_fungal_media 0.8X
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HSERO_RS18445 and HSERO_RS18450 are separated by 145 nucleotides HSERO_RS18450 and HSERO_RS18455 are separated by 87 nucleotides HSERO_RS18455 and HSERO_RS18460 are separated by 210 nucleotides
HSERO_RS18445: HSERO_RS18445 - membrane protein, at 4,233,226 to 4,233,837
_RS18445
HSERO_RS18450: HSERO_RS18450 - 3-demethylubiquinone-9 3-methyltransferase, at 4,233,983 to 4,234,708
_RS18450
HSERO_RS18455: HSERO_RS18455 - phosphoglycolate phosphatase, at 4,234,796 to 4,235,452
_RS18455
HSERO_RS18460: HSERO_RS18460 - hypothetical protein, at 4,235,663 to 4,239,037
_RS18460
Position (kb)
4234
4235
4236 Strain fitness (log2 ratio)
-1
0
1 at 4233.941 kb on - strand at 4234.702 kb on + strand at 4234.727 kb on + strand at 4234.807 kb on - strand at 4235.051 kb on + strand, within HSERO_RS18455 at 4235.309 kb on - strand, within HSERO_RS18455 at 4235.626 kb on - strand at 4235.659 kb on - strand at 4235.678 kb on - strand at 4235.819 kb on + strand at 4235.912 kb on - strand at 4236.112 kb on + strand, within HSERO_RS18460 at 4236.120 kb on - strand, within HSERO_RS18460 at 4236.338 kb on + strand, within HSERO_RS18460 at 4236.370 kb on - strand, within HSERO_RS18460
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Vogels_fungal_media 0.8X remove 4,233,941 - +0.2 4,234,702 + +0.2 4,234,727 + -0.0 4,234,807 - +0.8 4,235,051 + HSERO_RS18455 0.39 -0.4 4,235,309 - HSERO_RS18455 0.78 -1.3 4,235,626 - -0.2 4,235,659 - +0.0 4,235,678 - +0.1 4,235,819 + +0.1 4,235,912 - +0.3 4,236,112 + HSERO_RS18460 0.13 +0.1 4,236,120 - HSERO_RS18460 0.14 +0.0 4,236,338 + HSERO_RS18460 0.20 +0.7 4,236,370 - HSERO_RS18460 0.21 +0.1
Or see this region's nucleotide sequence