Experiment: R2A with Vogels_fungal_media 0.8X
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HSERO_RS16040 and HSERO_RS16045 overlap by 8 nucleotides HSERO_RS16045 and HSERO_RS16050 are separated by 4 nucleotides HSERO_RS16050 and HSERO_RS16055 are separated by 13 nucleotides
HSERO_RS16040: HSERO_RS16040 - cytochrome CBB3, at 3,662,818 to 3,663,750
_RS16040
HSERO_RS16045: HSERO_RS16045 - cytochrome oxidase, at 3,663,743 to 3,663,937
_RS16045
HSERO_RS16050: HSERO_RS16050 - peptidase S41, at 3,663,942 to 3,664,556
_RS16050
HSERO_RS16055: HSERO_RS16055 - cytochrome oxidase subunit I, at 3,664,570 to 3,665,997
_RS16055
Position (kb)
3663
3664
3665 Strain fitness (log2 ratio)
-1
0
1 at 3663.489 kb on + strand, within HSERO_RS16040 at 3663.601 kb on - strand, within HSERO_RS16040 at 3663.624 kb on + strand, within HSERO_RS16040 at 3663.766 kb on - strand, within HSERO_RS16045 at 3663.835 kb on - strand, within HSERO_RS16045 at 3664.086 kb on - strand, within HSERO_RS16050 at 3664.110 kb on - strand, within HSERO_RS16050 at 3664.403 kb on - strand, within HSERO_RS16050 at 3664.588 kb on - strand at 3664.614 kb on + strand at 3664.744 kb on + strand, within HSERO_RS16055 at 3664.745 kb on - strand, within HSERO_RS16055 at 3664.776 kb on + strand, within HSERO_RS16055 at 3664.988 kb on + strand, within HSERO_RS16055 at 3664.996 kb on - strand, within HSERO_RS16055 at 3665.103 kb on + strand, within HSERO_RS16055 at 3665.219 kb on + strand, within HSERO_RS16055 at 3665.233 kb on + strand, within HSERO_RS16055 at 3665.243 kb on + strand, within HSERO_RS16055 at 3665.243 kb on + strand, within HSERO_RS16055 at 3665.311 kb on + strand, within HSERO_RS16055 at 3665.443 kb on + strand, within HSERO_RS16055 at 3665.539 kb on - strand, within HSERO_RS16055 at 3665.544 kb on - strand, within HSERO_RS16055
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Vogels_fungal_media 0.8X remove 3,663,489 + HSERO_RS16040 0.72 -0.6 3,663,601 - HSERO_RS16040 0.84 -0.7 3,663,624 + HSERO_RS16040 0.86 -0.0 3,663,766 - HSERO_RS16045 0.12 +0.3 3,663,835 - HSERO_RS16045 0.47 +0.1 3,664,086 - HSERO_RS16050 0.23 +0.7 3,664,110 - HSERO_RS16050 0.27 -0.1 3,664,403 - HSERO_RS16050 0.75 -0.6 3,664,588 - -0.2 3,664,614 + +1.5 3,664,744 + HSERO_RS16055 0.12 -0.6 3,664,745 - HSERO_RS16055 0.12 +0.1 3,664,776 + HSERO_RS16055 0.14 +0.1 3,664,988 + HSERO_RS16055 0.29 -0.8 3,664,996 - HSERO_RS16055 0.30 -0.5 3,665,103 + HSERO_RS16055 0.37 -0.4 3,665,219 + HSERO_RS16055 0.45 -0.4 3,665,233 + HSERO_RS16055 0.46 -0.9 3,665,243 + HSERO_RS16055 0.47 +0.1 3,665,243 + HSERO_RS16055 0.47 +0.4 3,665,311 + HSERO_RS16055 0.52 -0.2 3,665,443 + HSERO_RS16055 0.61 +0.3 3,665,539 - HSERO_RS16055 0.68 -0.9 3,665,544 - HSERO_RS16055 0.68 +0.1
Or see this region's nucleotide sequence