Experiment: R2A with Vogels_fungal_media 0.8X
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HSERO_RS13405 and HSERO_RS13410 are separated by 63 nucleotides HSERO_RS13410 and HSERO_RS13415 are separated by 513 nucleotides HSERO_RS13415 and HSERO_RS13420 are separated by 181 nucleotides
HSERO_RS13405: HSERO_RS13405 - phosphodiesterase, at 3,052,491 to 3,053,711
_RS13405
HSERO_RS13410: HSERO_RS13410 - hypothetical protein, at 3,053,775 to 3,053,957
_RS13410
HSERO_RS13415: HSERO_RS13415 - VdlD, at 3,054,471 to 3,054,950
_RS13415
HSERO_RS13420: HSERO_RS13420 - 2-hydroxyacid dehydrogenase, at 3,055,132 to 3,056,064
_RS13420
Position (kb)
3054
3055 Strain fitness (log2 ratio)
-1
0
1 at 3053.489 kb on + strand, within HSERO_RS13405 at 3053.574 kb on + strand, within HSERO_RS13405 at 3053.884 kb on - strand, within HSERO_RS13410 at 3053.917 kb on + strand, within HSERO_RS13410 at 3053.939 kb on - strand at 3054.210 kb on + strand at 3054.341 kb on - strand at 3054.506 kb on + strand at 3054.727 kb on - strand, within HSERO_RS13415 at 3054.731 kb on + strand, within HSERO_RS13415 at 3054.738 kb on + strand, within HSERO_RS13415 at 3054.781 kb on - strand, within HSERO_RS13415 at 3054.804 kb on + strand at 3055.139 kb on - strand at 3055.258 kb on - strand, within HSERO_RS13420 at 3055.506 kb on + strand, within HSERO_RS13420 at 3055.858 kb on - strand, within HSERO_RS13420
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Vogels_fungal_media 0.8X remove 3,053,489 + HSERO_RS13405 0.82 -0.9 3,053,574 + HSERO_RS13405 0.89 +0.0 3,053,884 - HSERO_RS13410 0.60 +0.1 3,053,917 + HSERO_RS13410 0.78 -0.5 3,053,939 - +0.6 3,054,210 + -1.5 3,054,341 - +0.3 3,054,506 + -0.1 3,054,727 - HSERO_RS13415 0.53 -0.3 3,054,731 + HSERO_RS13415 0.54 -1.5 3,054,738 + HSERO_RS13415 0.56 -0.5 3,054,781 - HSERO_RS13415 0.65 -1.5 3,054,804 + -0.5 3,055,139 - +0.2 3,055,258 - HSERO_RS13420 0.14 -0.3 3,055,506 + HSERO_RS13420 0.40 +0.2 3,055,858 - HSERO_RS13420 0.78 -0.8
Or see this region's nucleotide sequence