Experiment: R2A with Vogels_fungal_media 0.8X
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HSERO_RS13290 and HSERO_RS13295 overlap by 1 nucleotides HSERO_RS13295 and HSERO_RS13300 overlap by 4 nucleotides HSERO_RS13300 and HSERO_RS13305 are separated by 11 nucleotides
HSERO_RS13290: HSERO_RS13290 - cobalt-precorrin-6A synthase, at 3,027,501 to 3,028,691
_RS13290
HSERO_RS13295: HSERO_RS13295 - membrane protein, at 3,028,691 to 3,030,268
_RS13295
HSERO_RS13300: HSERO_RS13300 - cobalt-precorrin-6Y C(5)-methyltransferase, at 3,030,265 to 3,030,990
_RS13300
HSERO_RS13305: HSERO_RS13305 - magnesium chelatase, at 3,031,002 to 3,032,993
_RS13305
Position (kb)
3028
3029
3030
3031 Strain fitness (log2 ratio)
-1
0
1
2 at 3028.040 kb on - strand, within HSERO_RS13290 at 3028.142 kb on + strand, within HSERO_RS13290 at 3028.244 kb on - strand at 3028.460 kb on - strand, within HSERO_RS13290 at 3028.549 kb on - strand, within HSERO_RS13290 at 3028.684 kb on - strand at 3029.062 kb on + strand, within HSERO_RS13295 at 3029.188 kb on - strand, within HSERO_RS13295 at 3029.487 kb on - strand, within HSERO_RS13295 at 3030.047 kb on - strand, within HSERO_RS13295 at 3030.324 kb on + strand at 3030.340 kb on - strand, within HSERO_RS13300 at 3030.444 kb on + strand, within HSERO_RS13300 at 3031.038 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Vogels_fungal_media 0.8X remove 3,028,040 - HSERO_RS13290 0.45 -0.1 3,028,142 + HSERO_RS13290 0.54 +0.4 3,028,244 - +1.9 3,028,460 - HSERO_RS13290 0.81 -0.3 3,028,549 - HSERO_RS13290 0.88 +0.0 3,028,684 - -0.2 3,029,062 + HSERO_RS13295 0.24 -0.1 3,029,188 - HSERO_RS13295 0.31 -0.3 3,029,487 - HSERO_RS13295 0.50 -0.6 3,030,047 - HSERO_RS13295 0.86 -1.4 3,030,324 + -0.1 3,030,340 - HSERO_RS13300 0.10 -0.2 3,030,444 + HSERO_RS13300 0.25 -0.3 3,031,038 - +0.3
Or see this region's nucleotide sequence