Experiment: R2A with Vogels_fungal_media 0.8X
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HSERO_RS11145 and HSERO_RS11150 are separated by 335 nucleotides HSERO_RS11150 and HSERO_RS11155 are separated by 199 nucleotides
HSERO_RS11145: HSERO_RS11145 - sulfate ABC transporter ATP-binding protein, at 2,515,278 to 2,516,357
_RS11145
HSERO_RS11150: HSERO_RS11150 - hypothetical protein, at 2,516,693 to 2,517,202
_RS11150
HSERO_RS11155: HSERO_RS11155 - peptidyl-prolyl cis-trans isomerase, at 2,517,402 to 2,519,321
_RS11155
Position (kb)
2516
2517
2518 Strain fitness (log2 ratio)
-1
0
1 at 2515.765 kb on - strand, within HSERO_RS11145 at 2515.769 kb on + strand, within HSERO_RS11145 at 2515.769 kb on + strand, within HSERO_RS11145 at 2515.919 kb on + strand, within HSERO_RS11145 at 2516.203 kb on - strand, within HSERO_RS11145 at 2516.271 kb on + strand at 2516.319 kb on + strand at 2516.546 kb on + strand at 2516.574 kb on + strand at 2516.672 kb on + strand at 2516.700 kb on + strand at 2516.708 kb on - strand at 2516.935 kb on + strand, within HSERO_RS11150 at 2517.050 kb on + strand, within HSERO_RS11150 at 2517.058 kb on - strand, within HSERO_RS11150 at 2517.103 kb on + strand, within HSERO_RS11150 at 2517.167 kb on - strand at 2517.233 kb on - strand at 2517.237 kb on + strand at 2517.245 kb on - strand at 2517.260 kb on + strand at 2517.280 kb on + strand at 2517.280 kb on + strand at 2517.364 kb on - strand at 2517.369 kb on + strand at 2517.798 kb on + strand, within HSERO_RS11155 at 2517.897 kb on + strand, within HSERO_RS11155 at 2517.953 kb on - strand, within HSERO_RS11155
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Vogels_fungal_media 0.8X remove 2,515,765 - HSERO_RS11145 0.45 +0.0 2,515,769 + HSERO_RS11145 0.45 -1.4 2,515,769 + HSERO_RS11145 0.45 -0.3 2,515,919 + HSERO_RS11145 0.59 -0.1 2,516,203 - HSERO_RS11145 0.86 -0.1 2,516,271 + +0.2 2,516,319 + -0.1 2,516,546 + -0.6 2,516,574 + +0.1 2,516,672 + -0.1 2,516,700 + -0.1 2,516,708 - +1.2 2,516,935 + HSERO_RS11150 0.47 -0.8 2,517,050 + HSERO_RS11150 0.70 -1.1 2,517,058 - HSERO_RS11150 0.72 +0.1 2,517,103 + HSERO_RS11150 0.80 -1.0 2,517,167 - -0.0 2,517,233 - -0.1 2,517,237 + +0.2 2,517,245 - +0.4 2,517,260 + -0.1 2,517,280 + +0.5 2,517,280 + +0.3 2,517,364 - -1.1 2,517,369 + -0.4 2,517,798 + HSERO_RS11155 0.21 -0.2 2,517,897 + HSERO_RS11155 0.26 -1.1 2,517,953 - HSERO_RS11155 0.29 +0.3
Or see this region's nucleotide sequence