Experiment: R2A with Vogels_fungal_media 0.8X
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HSERO_RS08335 and HSERO_RS08340 are separated by 147 nucleotides HSERO_RS08340 and HSERO_RS08345 are separated by 91 nucleotides HSERO_RS08345 and HSERO_RS08350 are separated by 83 nucleotides HSERO_RS08350 and HSERO_RS08355 overlap by 4 nucleotides
HSERO_RS08335: HSERO_RS08335 - 4-hydroxyphenylpyruvate dioxygenase, at 1,898,901 to 1,899,995
_RS08335
HSERO_RS08340: HSERO_RS08340 - AsnC family transcriptional regulator, at 1,900,143 to 1,900,661
_RS08340
HSERO_RS08345: HSERO_RS08345 - AraC family transcriptional regulator, at 1,900,753 to 1,901,862
_RS08345
HSERO_RS08350: HSERO_RS08350 - esterase, at 1,901,946 to 1,902,824
_RS08350
HSERO_RS08355: HSERO_RS08355 - dioxygenase, at 1,902,821 to 1,903,474
_RS08355
Position (kb)
1900
1901
1902 Strain fitness (log2 ratio)
-2
-1
0
1 at 1899.992 kb on - strand at 1900.352 kb on + strand, within HSERO_RS08340 at 1900.423 kb on + strand, within HSERO_RS08340 at 1900.473 kb on - strand, within HSERO_RS08340 at 1900.473 kb on - strand, within HSERO_RS08340 at 1900.580 kb on + strand, within HSERO_RS08340 at 1900.638 kb on - strand at 1900.651 kb on + strand at 1900.656 kb on + strand at 1901.040 kb on + strand, within HSERO_RS08345 at 1901.042 kb on - strand, within HSERO_RS08345 at 1901.230 kb on - strand, within HSERO_RS08345 at 1901.386 kb on + strand, within HSERO_RS08345 at 1901.429 kb on + strand, within HSERO_RS08345 at 1901.661 kb on - strand, within HSERO_RS08345 at 1901.721 kb on - strand, within HSERO_RS08345 at 1901.811 kb on + strand at 1901.930 kb on - strand at 1901.976 kb on + strand at 1902.123 kb on + strand, within HSERO_RS08350 at 1902.123 kb on + strand, within HSERO_RS08350 at 1902.313 kb on - strand, within HSERO_RS08350 at 1902.427 kb on + strand, within HSERO_RS08350 at 1902.460 kb on + strand, within HSERO_RS08350 at 1902.468 kb on - strand, within HSERO_RS08350 at 1902.567 kb on + strand, within HSERO_RS08350 at 1902.567 kb on + strand, within HSERO_RS08350 at 1902.591 kb on + strand, within HSERO_RS08350 at 1902.638 kb on + strand, within HSERO_RS08350 at 1902.720 kb on + strand, within HSERO_RS08350 at 1902.757 kb on + strand at 1902.767 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Vogels_fungal_media 0.8X remove 1,899,992 - -0.4 1,900,352 + HSERO_RS08340 0.40 +0.5 1,900,423 + HSERO_RS08340 0.54 +0.3 1,900,473 - HSERO_RS08340 0.64 -0.3 1,900,473 - HSERO_RS08340 0.64 +0.4 1,900,580 + HSERO_RS08340 0.84 -1.5 1,900,638 - +0.4 1,900,651 + +0.4 1,900,656 + +0.1 1,901,040 + HSERO_RS08345 0.26 -1.4 1,901,042 - HSERO_RS08345 0.26 -1.9 1,901,230 - HSERO_RS08345 0.43 -0.8 1,901,386 + HSERO_RS08345 0.57 -0.4 1,901,429 + HSERO_RS08345 0.61 +0.6 1,901,661 - HSERO_RS08345 0.82 -0.3 1,901,721 - HSERO_RS08345 0.87 -0.5 1,901,811 + -0.3 1,901,930 - -0.5 1,901,976 + +0.1 1,902,123 + HSERO_RS08350 0.20 -0.4 1,902,123 + HSERO_RS08350 0.20 +0.5 1,902,313 - HSERO_RS08350 0.42 -0.3 1,902,427 + HSERO_RS08350 0.55 +0.1 1,902,460 + HSERO_RS08350 0.58 +0.0 1,902,468 - HSERO_RS08350 0.59 -0.4 1,902,567 + HSERO_RS08350 0.71 -0.1 1,902,567 + HSERO_RS08350 0.71 +0.1 1,902,591 + HSERO_RS08350 0.73 -0.6 1,902,638 + HSERO_RS08350 0.79 +0.7 1,902,720 + HSERO_RS08350 0.88 +0.3 1,902,757 + -0.4 1,902,767 + +0.1
Or see this region's nucleotide sequence