Experiment: R2A with Vogels_fungal_media 0.8X
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HSERO_RS08110 and HSERO_RS08115 are separated by 114 nucleotides HSERO_RS08115 and HSERO_RS08120 are separated by 18 nucleotides HSERO_RS08120 and HSERO_RS08125 are separated by 309 nucleotides
HSERO_RS08110: HSERO_RS08110 - 2-hydroxyacid dehydrogenase, at 1,854,333 to 1,855,313
_RS08110
HSERO_RS08115: HSERO_RS08115 - acyl-CoA-binding protein, at 1,855,428 to 1,855,685
_RS08115
HSERO_RS08120: HSERO_RS08120 - peptidyl-prolyl cis-trans isomerase, at 1,855,704 to 1,856,060
_RS08120
HSERO_RS08125: HSERO_RS08125 - hypothetical protein, at 1,856,370 to 1,857,353
_RS08125
Position (kb)
1855
1856 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1854.460 kb on - strand, within HSERO_RS08110 at 1854.541 kb on - strand, within HSERO_RS08110 at 1854.543 kb on + strand, within HSERO_RS08110 at 1854.551 kb on - strand, within HSERO_RS08110 at 1854.604 kb on - strand, within HSERO_RS08110 at 1854.919 kb on + strand, within HSERO_RS08110 at 1854.967 kb on + strand, within HSERO_RS08110 at 1855.655 kb on - strand, within HSERO_RS08115 at 1856.038 kb on + strand at 1856.100 kb on + strand at 1856.105 kb on + strand at 1856.108 kb on - strand at 1856.110 kb on + strand at 1856.113 kb on - strand at 1856.195 kb on - strand at 1856.321 kb on + strand at 1856.654 kb on - strand, within HSERO_RS08125
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Vogels_fungal_media 0.8X remove 1,854,460 - HSERO_RS08110 0.13 +0.2 1,854,541 - HSERO_RS08110 0.21 +0.1 1,854,543 + HSERO_RS08110 0.21 +0.3 1,854,551 - HSERO_RS08110 0.22 -0.1 1,854,604 - HSERO_RS08110 0.28 +1.9 1,854,919 + HSERO_RS08110 0.60 -0.3 1,854,967 + HSERO_RS08110 0.65 -0.2 1,855,655 - HSERO_RS08115 0.88 -0.8 1,856,038 + -2.6 1,856,100 + -0.6 1,856,105 + -0.3 1,856,108 - +0.0 1,856,110 + +1.1 1,856,113 - -0.3 1,856,195 - +0.1 1,856,321 + +0.3 1,856,654 - HSERO_RS08125 0.29 -0.0
Or see this region's nucleotide sequence