Experiment: R2A with Vogels_fungal_media 0.8X
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HSERO_RS07245 and HSERO_RS07250 are separated by 123 nucleotides HSERO_RS07250 and HSERO_RS07255 are separated by 141 nucleotides HSERO_RS07255 and HSERO_RS07260 are separated by 315 nucleotides
HSERO_RS07245: HSERO_RS07245 - formate dehydrogenase, at 1,657,374 to 1,659,695
_RS07245
HSERO_RS07250: HSERO_RS07250 - DNA-binding protein, at 1,659,819 to 1,660,151
_RS07250
HSERO_RS07255: HSERO_RS07255 - glutaminase, at 1,660,293 to 1,661,363
_RS07255
HSERO_RS07260: HSERO_RS07260 - diguanylate cyclase, at 1,661,679 to 1,662,737
_RS07260
Position (kb)
1660
1661
1662 Strain fitness (log2 ratio)
-2
-1
0
1 at 1659.323 kb on + strand, within HSERO_RS07245 at 1659.367 kb on - strand, within HSERO_RS07245 at 1659.514 kb on + strand at 1659.577 kb on - strand at 1659.697 kb on - strand at 1659.697 kb on - strand at 1659.697 kb on - strand at 1659.697 kb on - strand at 1659.697 kb on - strand at 1660.047 kb on - strand, within HSERO_RS07250 at 1660.117 kb on + strand, within HSERO_RS07250 at 1660.129 kb on + strand at 1660.319 kb on - strand at 1660.617 kb on + strand, within HSERO_RS07255 at 1660.625 kb on - strand, within HSERO_RS07255 at 1660.735 kb on - strand, within HSERO_RS07255 at 1660.834 kb on - strand, within HSERO_RS07255 at 1661.322 kb on + strand at 1661.330 kb on - strand at 1661.422 kb on - strand at 1661.576 kb on + strand at 1661.658 kb on - strand at 1661.707 kb on - strand at 1661.739 kb on - strand at 1662.198 kb on - strand, within HSERO_RS07260 at 1662.247 kb on + strand, within HSERO_RS07260
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Vogels_fungal_media 0.8X remove 1,659,323 + HSERO_RS07245 0.84 +0.2 1,659,367 - HSERO_RS07245 0.86 -0.5 1,659,514 + -1.9 1,659,577 - +0.0 1,659,697 - -0.0 1,659,697 - -0.2 1,659,697 - +0.1 1,659,697 - -0.6 1,659,697 - +0.1 1,660,047 - HSERO_RS07250 0.68 +0.3 1,660,117 + HSERO_RS07250 0.89 -0.5 1,660,129 + -0.2 1,660,319 - -0.2 1,660,617 + HSERO_RS07255 0.30 +0.3 1,660,625 - HSERO_RS07255 0.31 -0.2 1,660,735 - HSERO_RS07255 0.41 -1.0 1,660,834 - HSERO_RS07255 0.51 -0.6 1,661,322 + +0.2 1,661,330 - -0.5 1,661,422 - -0.3 1,661,576 + -0.7 1,661,658 - -0.3 1,661,707 - +0.9 1,661,739 - -0.0 1,662,198 - HSERO_RS07260 0.49 -0.2 1,662,247 + HSERO_RS07260 0.54 +0.3
Or see this region's nucleotide sequence