Experiment: R2A with Vogels_fungal_media 0.8X
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HSERO_RS05900 and HSERO_RS05905 are separated by 122 nucleotides HSERO_RS05905 and HSERO_RS05910 are separated by 72 nucleotides HSERO_RS05910 and HSERO_RS05915 are separated by 51 nucleotides
HSERO_RS05900: HSERO_RS05900 - ribonuclease, at 1,342,022 to 1,342,417
_RS05900
HSERO_RS05905: HSERO_RS05905 - barnase inhibitor, at 1,342,540 to 1,342,938
_RS05905
HSERO_RS05910: HSERO_RS05910 - phosphoribosylglycinamide formyltransferase, at 1,343,011 to 1,343,673
_RS05910
HSERO_RS05915: HSERO_RS05915 - SAM-dependent methyltransferase, at 1,343,725 to 1,344,984
_RS05915
Position (kb)
1343
1344 Strain fitness (log2 ratio)
-2
-1
0
1 at 1342.113 kb on + strand, within HSERO_RS05900 at 1342.143 kb on + strand, within HSERO_RS05900 at 1342.176 kb on + strand, within HSERO_RS05900 at 1342.184 kb on - strand, within HSERO_RS05900 at 1342.464 kb on + strand at 1342.464 kb on + strand at 1342.477 kb on - strand at 1342.511 kb on - strand at 1342.516 kb on - strand at 1342.563 kb on + strand at 1342.576 kb on - strand at 1343.166 kb on + strand, within HSERO_RS05910 at 1343.286 kb on + strand, within HSERO_RS05910 at 1343.831 kb on + strand at 1343.912 kb on + strand, within HSERO_RS05915 at 1343.975 kb on - strand, within HSERO_RS05915 at 1343.984 kb on - strand at 1344.134 kb on + strand, within HSERO_RS05915 at 1344.144 kb on + strand, within HSERO_RS05915 at 1344.326 kb on - strand, within HSERO_RS05915 at 1344.326 kb on - strand, within HSERO_RS05915
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Vogels_fungal_media 0.8X remove 1,342,113 + HSERO_RS05900 0.23 -1.5 1,342,143 + HSERO_RS05900 0.31 +0.5 1,342,176 + HSERO_RS05900 0.39 -0.1 1,342,184 - HSERO_RS05900 0.41 -0.6 1,342,464 + -0.6 1,342,464 + -0.1 1,342,477 - -0.5 1,342,511 - -0.5 1,342,516 - -0.1 1,342,563 + +0.2 1,342,576 - +0.0 1,343,166 + HSERO_RS05910 0.23 -2.3 1,343,286 + HSERO_RS05910 0.41 -1.5 1,343,831 + -0.5 1,343,912 + HSERO_RS05915 0.15 -1.2 1,343,975 - HSERO_RS05915 0.20 -0.3 1,343,984 - +0.3 1,344,134 + HSERO_RS05915 0.32 -0.7 1,344,144 + HSERO_RS05915 0.33 -0.3 1,344,326 - HSERO_RS05915 0.48 -0.7 1,344,326 - HSERO_RS05915 0.48 +0.9
Or see this region's nucleotide sequence