Experiment: R2A with Vogels_fungal_media 0.8X
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HSERO_RS03870 and HSERO_RS03875 are separated by 587 nucleotides HSERO_RS03875 and HSERO_RS03880 are separated by 61 nucleotides HSERO_RS03880 and HSERO_RS03885 are separated by 257 nucleotides
HSERO_RS03870: HSERO_RS03870 - hypothetical protein, at 854,896 to 855,240
_RS03870
HSERO_RS03875: HSERO_RS03875 - hypothetical protein, at 855,828 to 856,037
_RS03875
HSERO_RS03880: HSERO_RS03880 - hypothetical protein, at 856,099 to 856,281
_RS03880
HSERO_RS03885: HSERO_RS03885 - hypothetical protein, at 856,539 to 858,266
_RS03885
Position (kb) 855
856
857 Strain fitness (log2 ratio) -1
0
1 at 854.951 kb on + strand, within HSERO_RS03870 at 854.959 kb on - strand, within HSERO_RS03870 at 855.117 kb on - strand, within HSERO_RS03870 at 855.175 kb on - strand at 855.365 kb on - strand at 855.365 kb on - strand at 855.437 kb on + strand at 855.471 kb on - strand at 855.545 kb on + strand at 855.658 kb on - strand at 855.735 kb on - strand at 855.928 kb on + strand, within HSERO_RS03875 at 855.953 kb on + strand, within HSERO_RS03875 at 856.260 kb on - strand, within HSERO_RS03880 at 856.566 kb on + strand at 856.574 kb on - strand at 856.762 kb on - strand, within HSERO_RS03885 at 856.762 kb on - strand, within HSERO_RS03885 at 856.845 kb on + strand, within HSERO_RS03885 at 857.032 kb on - strand, within HSERO_RS03885
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Vogels_fungal_media 0.8X remove 854,951 + HSERO_RS03870 0.16 +0.2 854,959 - HSERO_RS03870 0.18 -0.2 855,117 - HSERO_RS03870 0.64 +0.1 855,175 - -1.0 855,365 - -0.1 855,365 - -1.1 855,437 + -0.2 855,471 - -0.8 855,545 + +0.1 855,658 - +1.0 855,735 - +0.6 855,928 + HSERO_RS03875 0.48 +0.6 855,953 + HSERO_RS03875 0.60 -1.1 856,260 - HSERO_RS03880 0.88 +0.6 856,566 + +0.5 856,574 - +1.7 856,762 - HSERO_RS03885 0.13 +0.1 856,762 - HSERO_RS03885 0.13 -0.1 856,845 + HSERO_RS03885 0.18 -0.3 857,032 - HSERO_RS03885 0.29 +0.3
Or see this region's nucleotide sequence