Experiment: R2A with Vogels_fungal_media 0.8X
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HSERO_RS03535 and HSERO_RS03540 are separated by 26 nucleotides HSERO_RS03540 and HSERO_RS03545 are separated by 197 nucleotides HSERO_RS03545 and HSERO_RS03550 are separated by 201 nucleotides
HSERO_RS03535: HSERO_RS03535 - 4-hydroxythreonine-4-phosphate dehydrogenase, at 770,303 to 771,325
_RS03535
HSERO_RS03540: HSERO_RS03540 - dimethyladenosine transferase, at 771,352 to 772,134
_RS03540
HSERO_RS03545: HSERO_RS03545 - hypothetical protein, at 772,332 to 772,709
_RS03545
HSERO_RS03550: HSERO_RS03550 - glyoxalase I, at 772,911 to 773,315
_RS03550
Position (kb) 771
772
773 Strain fitness (log2 ratio) -1
0
1 at 771.575 kb on - strand, within HSERO_RS03540 at 771.951 kb on + strand, within HSERO_RS03540 at 772.250 kb on + strand at 772.304 kb on - strand at 772.397 kb on - strand, within HSERO_RS03545 at 772.413 kb on + strand, within HSERO_RS03545 at 772.557 kb on + strand, within HSERO_RS03545 at 772.656 kb on - strand, within HSERO_RS03545 at 772.746 kb on - strand at 772.773 kb on - strand at 772.793 kb on + strand at 772.794 kb on + strand at 772.802 kb on - strand at 772.980 kb on - strand, within HSERO_RS03550 at 772.992 kb on - strand, within HSERO_RS03550
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Vogels_fungal_media 0.8X remove 771,575 - HSERO_RS03540 0.28 +0.0 771,951 + HSERO_RS03540 0.77 -1.4 772,250 + +0.7 772,304 - +0.4 772,397 - HSERO_RS03545 0.17 +0.2 772,413 + HSERO_RS03545 0.21 -0.4 772,557 + HSERO_RS03545 0.60 -0.1 772,656 - HSERO_RS03545 0.86 +0.2 772,746 - -0.0 772,773 - +0.0 772,793 + +0.6 772,794 + +0.1 772,802 - -0.5 772,980 - HSERO_RS03550 0.17 +0.0 772,992 - HSERO_RS03550 0.20 -0.9
Or see this region's nucleotide sequence