Experiment: R2A with Vogels_fungal_media 0.8X
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HSERO_RS01870 and HSERO_RS01875 overlap by 4 nucleotides HSERO_RS01875 and HSERO_RS01880 are separated by 3 nucleotides HSERO_RS01880 and HSERO_RS01885 are separated by 406 nucleotides
HSERO_RS01870: HSERO_RS01870 - ABC transporter permease, at 410,664 to 411,476
_RS01870
HSERO_RS01875: HSERO_RS01875 - hypothetical protein, at 411,473 to 411,757
_RS01875
HSERO_RS01880: HSERO_RS01880 - N-acetyl-anhydromuranmyl-L-alanine amidase, at 411,761 to 412,384
_RS01880
HSERO_RS01885: HSERO_RS01885 - ribonucleotide-diphosphate reductase subunit alpha, at 412,791 to 415,775
_RS01885
Position (kb)
411
412
413 Strain fitness (log2 ratio)
-2
-1
0
1 at 410.789 kb on + strand, within HSERO_RS01870 at 411.213 kb on - strand, within HSERO_RS01870 at 411.253 kb on + strand, within HSERO_RS01870 at 411.385 kb on + strand, within HSERO_RS01870 at 411.385 kb on + strand, within HSERO_RS01870 at 411.687 kb on + strand, within HSERO_RS01875 at 411.713 kb on + strand, within HSERO_RS01875 at 411.949 kb on - strand, within HSERO_RS01880 at 412.163 kb on + strand, within HSERO_RS01880 at 412.257 kb on + strand, within HSERO_RS01880 at 412.267 kb on + strand, within HSERO_RS01880 at 412.356 kb on + strand at 412.595 kb on - strand at 412.654 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Vogels_fungal_media 0.8X remove 410,789 + HSERO_RS01870 0.15 +0.4 411,213 - HSERO_RS01870 0.68 -0.2 411,253 + HSERO_RS01870 0.72 +0.0 411,385 + HSERO_RS01870 0.89 -0.7 411,385 + HSERO_RS01870 0.89 -1.0 411,687 + HSERO_RS01875 0.75 -0.1 411,713 + HSERO_RS01875 0.84 +0.2 411,949 - HSERO_RS01880 0.30 -0.2 412,163 + HSERO_RS01880 0.64 -2.7 412,257 + HSERO_RS01880 0.79 -0.5 412,267 + HSERO_RS01880 0.81 -1.3 412,356 + +0.8 412,595 - -0.4 412,654 - -2.6
Or see this region's nucleotide sequence