Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS26225

Experiment: Fungal_growth_media_mCAFEs_v1 1x with Mixed culture; Trichoderma harzianum M10 spores at 2.5*10^6 spores/mL

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS26220 and BWI76_RS26225 are separated by 358 nucleotidesBWI76_RS26225 and BWI76_RS26230 overlap by 4 nucleotides BWI76_RS26220: BWI76_RS26220 - aspartate-semialdehyde dehydrogenase, at 5,426,671 to 5,427,777 _RS26220 BWI76_RS26225: BWI76_RS26225 - DNA processing protein DprA, at 5,428,136 to 5,429,341 _RS26225 BWI76_RS26230: BWI76_RS26230 - ATP-dependent DNA helicase, at 5,429,338 to 5,431,437 _RS26230 Position (kb) 5428 5429 5430Strain fitness (log2 ratio) -3 -2 -1 0 1 2 3at 5427.801 kb on - strandat 5427.826 kb on - strandat 5427.836 kb on - strandat 5428.144 kb on + strandat 5428.152 kb on - strandat 5428.152 kb on - strandat 5428.184 kb on + strandat 5428.200 kb on - strandat 5428.206 kb on + strandat 5428.206 kb on + strandat 5428.206 kb on + strandat 5428.206 kb on + strandat 5428.206 kb on + strandat 5428.206 kb on + strandat 5428.206 kb on + strandat 5428.206 kb on + strandat 5428.214 kb on - strandat 5428.214 kb on - strandat 5428.214 kb on - strandat 5428.214 kb on - strandat 5428.214 kb on - strandat 5428.214 kb on - strandat 5428.228 kb on + strandat 5428.228 kb on + strandat 5428.228 kb on + strandat 5428.228 kb on + strandat 5428.228 kb on + strandat 5428.228 kb on + strandat 5428.236 kb on - strandat 5428.388 kb on + strand, within BWI76_RS26225at 5428.407 kb on + strand, within BWI76_RS26225at 5428.530 kb on - strand, within BWI76_RS26225at 5428.569 kb on - strand, within BWI76_RS26225at 5428.714 kb on - strand, within BWI76_RS26225at 5428.822 kb on + strand, within BWI76_RS26225at 5428.822 kb on + strand, within BWI76_RS26225at 5428.944 kb on - strand, within BWI76_RS26225at 5429.056 kb on + strand, within BWI76_RS26225at 5429.056 kb on + strand, within BWI76_RS26225at 5429.062 kb on + strand, within BWI76_RS26225at 5429.098 kb on + strand, within BWI76_RS26225at 5429.179 kb on + strand, within BWI76_RS26225at 5429.276 kb on + strandat 5429.284 kb on - strandat 5429.479 kb on - strandat 5429.644 kb on - strand, within BWI76_RS26230at 5430.001 kb on - strand, within BWI76_RS26230at 5430.017 kb on + strand, within BWI76_RS26230at 5430.031 kb on - strand, within BWI76_RS26230at 5430.063 kb on + strand, within BWI76_RS26230at 5430.071 kb on + strand, within BWI76_RS26230at 5430.071 kb on + strand, within BWI76_RS26230at 5430.079 kb on - strand, within BWI76_RS26230at 5430.079 kb on - strand, within BWI76_RS26230at 5430.159 kb on - strand, within BWI76_RS26230at 5430.247 kb on + strand, within BWI76_RS26230at 5430.249 kb on + strand, within BWI76_RS26230at 5430.255 kb on - strand, within BWI76_RS26230at 5430.287 kb on - strand, within BWI76_RS26230at 5430.315 kb on + strand, within BWI76_RS26230

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Per-strain Table

Position Strand Gene LocusTag Fraction Fungal_growth_media_mCAFEs_v1 1x with Mixed culture; Trichoderma harzianum M10 spores at 2.5*10^6 spores/mL
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5,427,801 - -3.3
5,427,826 - +1.0
5,427,836 - +0.6
5,428,144 + +1.3
5,428,152 - -0.8
5,428,152 - -0.0
5,428,184 + +3.3
5,428,200 - +0.4
5,428,206 + -2.0
5,428,206 + +1.9
5,428,206 + +0.7
5,428,206 + -1.8
5,428,206 + -0.5
5,428,206 + +0.6
5,428,206 + -0.0
5,428,206 + -1.0
5,428,214 - +0.8
5,428,214 - +0.6
5,428,214 - +1.0
5,428,214 - -0.0
5,428,214 - +1.0
5,428,214 - -1.1
5,428,228 + +0.3
5,428,228 + -0.4
5,428,228 + +0.6
5,428,228 + -0.6
5,428,228 + -2.8
5,428,228 + +0.3
5,428,236 - +0.3
5,428,388 + BWI76_RS26225 0.21 -2.9
5,428,407 + BWI76_RS26225 0.22 -0.8
5,428,530 - BWI76_RS26225 0.33 -1.6
5,428,569 - BWI76_RS26225 0.36 +0.7
5,428,714 - BWI76_RS26225 0.48 +0.4
5,428,822 + BWI76_RS26225 0.57 -1.3
5,428,822 + BWI76_RS26225 0.57 +0.1
5,428,944 - BWI76_RS26225 0.67 -1.5
5,429,056 + BWI76_RS26225 0.76 -1.0
5,429,056 + BWI76_RS26225 0.76 +1.4
5,429,062 + BWI76_RS26225 0.77 -2.2
5,429,098 + BWI76_RS26225 0.80 +0.7
5,429,179 + BWI76_RS26225 0.86 -0.8
5,429,276 + -2.5
5,429,284 - -0.6
5,429,479 - +0.6
5,429,644 - BWI76_RS26230 0.15 +0.6
5,430,001 - BWI76_RS26230 0.32 +1.1
5,430,017 + BWI76_RS26230 0.32 -2.4
5,430,031 - BWI76_RS26230 0.33 -0.2
5,430,063 + BWI76_RS26230 0.35 +0.7
5,430,071 + BWI76_RS26230 0.35 +1.7
5,430,071 + BWI76_RS26230 0.35 -1.8
5,430,079 - BWI76_RS26230 0.35 +2.6
5,430,079 - BWI76_RS26230 0.35 -1.2
5,430,159 - BWI76_RS26230 0.39 -0.5
5,430,247 + BWI76_RS26230 0.43 +0.5
5,430,249 + BWI76_RS26230 0.43 +0.8
5,430,255 - BWI76_RS26230 0.44 +0.9
5,430,287 - BWI76_RS26230 0.45 -0.6
5,430,315 + BWI76_RS26230 0.47 +0.8

Or see this region's nucleotide sequence