Experiment: Fungal_growth_media_mCAFEs_v1 1x with Mixed culture; Trichoderma harzianum M10 spores at 2.5*10^6 spores/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS09220 and BWI76_RS09225 overlap by 37 nucleotides BWI76_RS09225 and BWI76_RS09230 are separated by 67 nucleotides BWI76_RS09230 and BWI76_RS09235 overlap by 1 nucleotides BWI76_RS09235 and BWI76_RS09240 overlap by 4 nucleotides BWI76_RS09240 and BWI76_RS09245 overlap by 4 nucleotides
BWI76_RS09220: BWI76_RS09220 - hypothetical protein, at 1,944,068 to 1,944,268
_RS09220
BWI76_RS09225: BWI76_RS09225 - hypothetical protein, at 1,944,232 to 1,944,573
_RS09225
BWI76_RS09230: BWI76_RS09230 - hypothetical protein, at 1,944,641 to 1,944,877
_RS09230
BWI76_RS09235: BWI76_RS09235 - TraR/DksA family transcriptional regulator, at 1,944,877 to 1,945,104
_RS09235
BWI76_RS09240: BWI76_RS09240 - phosphoadenosine phosphosulfate reductase, at 1,945,101 to 1,946,111
_RS09240
BWI76_RS09245: BWI76_RS09245 - bacteriophage replication gene A protein, at 1,946,108 to 1,948,519
_RS09245
Position (kb)
1945
1946
1947 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 1944.131 kb on + strand, within BWI76_RS09220 at 1944.154 kb on + strand, within BWI76_RS09220 at 1944.155 kb on + strand, within BWI76_RS09220 at 1944.178 kb on - strand, within BWI76_RS09220 at 1944.198 kb on + strand, within BWI76_RS09220 at 1944.206 kb on - strand, within BWI76_RS09220 at 1944.221 kb on - strand, within BWI76_RS09220 at 1944.246 kb on - strand at 1944.259 kb on + strand at 1944.267 kb on - strand at 1944.267 kb on - strand at 1944.269 kb on + strand, within BWI76_RS09225 at 1944.269 kb on + strand, within BWI76_RS09225 at 1944.269 kb on + strand, within BWI76_RS09225 at 1944.274 kb on + strand at 1944.277 kb on - strand, within BWI76_RS09225 at 1944.277 kb on - strand, within BWI76_RS09225 at 1944.290 kb on + strand, within BWI76_RS09225 at 1944.302 kb on - strand, within BWI76_RS09225 at 1944.322 kb on - strand, within BWI76_RS09225 at 1944.469 kb on - strand, within BWI76_RS09225 at 1944.478 kb on - strand, within BWI76_RS09225 at 1944.485 kb on - strand, within BWI76_RS09225 at 1944.485 kb on - strand, within BWI76_RS09225 at 1944.490 kb on - strand, within BWI76_RS09225 at 1944.502 kb on - strand, within BWI76_RS09225 at 1944.504 kb on + strand, within BWI76_RS09225 at 1944.504 kb on + strand, within BWI76_RS09225 at 1944.512 kb on - strand, within BWI76_RS09225 at 1944.512 kb on - strand, within BWI76_RS09225 at 1944.540 kb on - strand at 1944.804 kb on + strand, within BWI76_RS09230 at 1944.812 kb on - strand, within BWI76_RS09230 at 1944.852 kb on - strand, within BWI76_RS09230 at 1944.893 kb on + strand at 1944.896 kb on + strand at 1945.182 kb on - strand at 1945.182 kb on - strand at 1945.218 kb on - strand, within BWI76_RS09240 at 1945.331 kb on + strand, within BWI76_RS09240 at 1945.361 kb on + strand at 1945.535 kb on + strand, within BWI76_RS09240 at 1945.606 kb on + strand, within BWI76_RS09240 at 1946.216 kb on + strand at 1946.235 kb on + strand at 1946.370 kb on + strand, within BWI76_RS09245 at 1946.378 kb on - strand, within BWI76_RS09245 at 1946.440 kb on + strand, within BWI76_RS09245 at 1946.467 kb on - strand, within BWI76_RS09245 at 1946.586 kb on + strand, within BWI76_RS09245 at 1946.594 kb on - strand, within BWI76_RS09245 at 1946.604 kb on - strand, within BWI76_RS09245 at 1946.657 kb on + strand, within BWI76_RS09245 at 1946.701 kb on + strand, within BWI76_RS09245 at 1946.985 kb on + strand, within BWI76_RS09245 at 1947.111 kb on + strand, within BWI76_RS09245
Per-strain Table
Position Strand Gene LocusTag Fraction Fungal_growth_media_mCAFEs_v1 1x with Mixed culture; Trichoderma harzianum M10 spores at 2.5*10^6 spores/mL remove 1,944,131 + BWI76_RS09220 0.31 +0.4 1,944,154 + BWI76_RS09220 0.43 +1.6 1,944,155 + BWI76_RS09220 0.43 +0.1 1,944,178 - BWI76_RS09220 0.55 -0.7 1,944,198 + BWI76_RS09220 0.65 -1.2 1,944,206 - BWI76_RS09220 0.69 +0.3 1,944,221 - BWI76_RS09220 0.76 +1.6 1,944,246 - +0.3 1,944,259 + -0.1 1,944,267 - -0.5 1,944,267 - +0.5 1,944,269 + BWI76_RS09225 0.11 -2.8 1,944,269 + BWI76_RS09225 0.11 +0.4 1,944,269 + BWI76_RS09225 0.11 -1.3 1,944,274 + -0.6 1,944,277 - BWI76_RS09225 0.13 +0.3 1,944,277 - BWI76_RS09225 0.13 -1.7 1,944,290 + BWI76_RS09225 0.17 -2.5 1,944,302 - BWI76_RS09225 0.20 -0.0 1,944,322 - BWI76_RS09225 0.26 +0.7 1,944,469 - BWI76_RS09225 0.69 +0.7 1,944,478 - BWI76_RS09225 0.72 -0.4 1,944,485 - BWI76_RS09225 0.74 +1.5 1,944,485 - BWI76_RS09225 0.74 -2.3 1,944,490 - BWI76_RS09225 0.75 +1.4 1,944,502 - BWI76_RS09225 0.79 +2.2 1,944,504 + BWI76_RS09225 0.80 +0.5 1,944,504 + BWI76_RS09225 0.80 -0.2 1,944,512 - BWI76_RS09225 0.82 -0.0 1,944,512 - BWI76_RS09225 0.82 +1.9 1,944,540 - -3.1 1,944,804 + BWI76_RS09230 0.69 +0.2 1,944,812 - BWI76_RS09230 0.72 -1.7 1,944,852 - BWI76_RS09230 0.89 -0.4 1,944,893 + -0.5 1,944,896 + -2.8 1,945,182 - -0.8 1,945,182 - +0.1 1,945,218 - BWI76_RS09240 0.12 +1.3 1,945,331 + BWI76_RS09240 0.23 -0.0 1,945,361 + -0.0 1,945,535 + BWI76_RS09240 0.43 +0.4 1,945,606 + BWI76_RS09240 0.50 +1.7 1,946,216 + +0.9 1,946,235 + -1.4 1,946,370 + BWI76_RS09245 0.11 -0.9 1,946,378 - BWI76_RS09245 0.11 +0.3 1,946,440 + BWI76_RS09245 0.14 +0.2 1,946,467 - BWI76_RS09245 0.15 -0.5 1,946,586 + BWI76_RS09245 0.20 -2.5 1,946,594 - BWI76_RS09245 0.20 -0.2 1,946,604 - BWI76_RS09245 0.21 -0.8 1,946,657 + BWI76_RS09245 0.23 -1.3 1,946,701 + BWI76_RS09245 0.25 -0.8 1,946,985 + BWI76_RS09245 0.36 -1.8 1,947,111 + BWI76_RS09245 0.42 +0.4
Or see this region's nucleotide sequence