Experiment: R2A with Vogels_fungal_media 0.2X
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HSERO_RS21750 and HSERO_RS21755 are separated by 33 nucleotides HSERO_RS21755 and HSERO_RS21760 are separated by 97 nucleotides HSERO_RS21760 and HSERO_RS21765 are separated by 107 nucleotides HSERO_RS21765 and HSERO_RS21770 are separated by 21 nucleotides
HSERO_RS21750: HSERO_RS21750 - methionyl-tRNA formyltransferase, at 4,981,098 to 4,982,051
_RS21750
HSERO_RS21755: HSERO_RS21755 - short-chain dehydrogenase, at 4,982,085 to 4,982,891
_RS21755
HSERO_RS21760: HSERO_RS21760 - DSBA oxidoreductase, at 4,982,989 to 4,983,678
_RS21760
HSERO_RS21765: HSERO_RS21765 - cell division protein, at 4,983,786 to 4,984,445
_RS21765
HSERO_RS21770: HSERO_RS21770 - arginyl-tRNA synthetase, at 4,984,467 to 4,986,200
_RS21770
Position (kb) 4982
4983
4984 Strain fitness (log2 ratio) -1
0
1 at 4982.091 kb on - strand at 4982.147 kb on - strand at 4982.210 kb on + strand, within HSERO_RS21755 at 4982.218 kb on - strand, within HSERO_RS21755 at 4982.225 kb on - strand, within HSERO_RS21755 at 4982.309 kb on - strand, within HSERO_RS21755 at 4982.394 kb on - strand, within HSERO_RS21755 at 4982.518 kb on - strand, within HSERO_RS21755 at 4982.530 kb on + strand, within HSERO_RS21755 at 4982.735 kb on - strand, within HSERO_RS21755 at 4982.786 kb on - strand, within HSERO_RS21755 at 4982.818 kb on + strand at 4982.906 kb on - strand at 4982.987 kb on + strand at 4983.097 kb on + strand, within HSERO_RS21760 at 4983.149 kb on + strand, within HSERO_RS21760 at 4983.182 kb on - strand, within HSERO_RS21760 at 4983.570 kb on + strand, within HSERO_RS21760 at 4983.680 kb on + strand at 4983.680 kb on + strand at 4983.688 kb on - strand at 4983.688 kb on - strand at 4983.729 kb on + strand at 4983.883 kb on - strand, within HSERO_RS21765 at 4984.074 kb on + strand, within HSERO_RS21765 at 4984.132 kb on + strand, within HSERO_RS21765 at 4984.178 kb on + strand, within HSERO_RS21765 at 4984.325 kb on - strand, within HSERO_RS21765
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Vogels_fungal_media 0.2X remove 4,982,091 - -0.4 4,982,147 - +1.2 4,982,210 + HSERO_RS21755 0.15 -0.1 4,982,218 - HSERO_RS21755 0.16 +0.2 4,982,225 - HSERO_RS21755 0.17 +0.3 4,982,309 - HSERO_RS21755 0.28 -0.1 4,982,394 - HSERO_RS21755 0.38 +0.2 4,982,518 - HSERO_RS21755 0.54 -0.5 4,982,530 + HSERO_RS21755 0.55 +0.0 4,982,735 - HSERO_RS21755 0.81 -0.7 4,982,786 - HSERO_RS21755 0.87 -0.1 4,982,818 + -0.1 4,982,906 - -0.6 4,982,987 + -0.5 4,983,097 + HSERO_RS21760 0.16 +1.2 4,983,149 + HSERO_RS21760 0.23 +0.4 4,983,182 - HSERO_RS21760 0.28 +0.3 4,983,570 + HSERO_RS21760 0.84 -0.2 4,983,680 + +0.2 4,983,680 + +0.1 4,983,688 - +0.2 4,983,688 - +1.5 4,983,729 + -0.4 4,983,883 - HSERO_RS21765 0.15 +0.5 4,984,074 + HSERO_RS21765 0.44 +0.1 4,984,132 + HSERO_RS21765 0.52 -0.1 4,984,178 + HSERO_RS21765 0.59 +1.2 4,984,325 - HSERO_RS21765 0.82 +0.7
Or see this region's nucleotide sequence